Abbkine Scientific Co., Ltd.
KTE70775
$260.00
48T
| 48T - | $260.00 | |
| 96T - | $450.00 | |
| 96T*5 - | $2,025.00 | |
| 96T*50 - | $18,900.00 |
Mouse Podoplanin (PDPN) ELISA Kit
Fig.1. Mouse Podoplanin (PDPN) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Podoplanin
Synonyms: Glycoprotein 38; OTS-8; PA2.26 antigen; Transmembrane glycoprotein E11; PDPN_MOUSE; A2A8J3; Q546R8; Q61612; Q62011
Description:
Podoplanin is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
PDPN
CTA-520D8.1
AGGRUS
GP36
GP40
Gp38
HT1A-1
OTS8
PA2.26
T1A
T1A-2
glycoprotein
36-KD
hT1alpha-1
hT1alpha-2
lung type I cell membrane associated glycoprotein
lung type-I cell membrane-associa
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.061 ng/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Mouse Podoplanin microplate
• Mouse Podoplanin standard
• Mouse Podoplanin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:0.156 - 10ng/mL