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48T -$260.00
96T -$450.00
96T*5 -$2,025.00
96T*50 -$18,900.00
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Mouse POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit
POU domain, class 5, transcription factor 1
Synonyms: NF-A3; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; PO5F1_MOUSE; P20263; Q63843
Oct-4 is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells. Oct-4 expression must be closely regulated; too much or too little will actually cause differentiation of the cells. Oct-4 transcription factor is initially active as a maternal factor in the oocyte but remains active in embryos throughout the preimplantation period. Oct-4 expression is associated with an undifferentiated phenotype and tumors. In fact gene knockdown of Oct-4 promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Test method:Colorimetric
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
POU domain
class 5
transcription factor 1
POU-type homeodomain-containing DNA-binding protein
octamer-binding transcription factor-3
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.061 ng/mL
Sample type:
Cell culture supernatants
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Mouse POU domain, class 5, transcription factor 1 microplate
• Mouse POU domain, class 5, transcription factor 1 standard
• Mouse POU domain, class 5, transcription factor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:0.156 - 10ng/mL