Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000 ELISA 1:5000-20000.
Purification:The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Phosphatidylcholine translocator ABCB4
ATP-binding cassette sub-family B member 4
Multidrug resistance protein 3
Buffer form:PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Storage conditions:Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Delivery conditions:Gel pack with blue ice.
Immunogen:Synthesized peptide derived from part region of human MDR3 protein at amino acid sequence of 20-100
ABCB4 (ATP Binding Cassette Subfamily B Member 4) is a Protein Coding gene. Diseases associated with ABCB4 include Cholestasis, Progressive Familial Intrahepatic 3 and Gallbladder Disease 1. Among its related pathways are Nuclear Receptors in Lipid Metabolism and Toxicity and Hepatic ABC Transporters. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate.