Abbkine Scientific Co., Ltd.
KTE61616
$305.00
48T
| 48T - | $305.00 | |
| 96T - | $570.00 | |
| 96T*5 - | $2,565.00 | |
| 96T*50 - | $22,800.00 |
Human Macrophage Migration Inhibitory Factor (MIF) ELISA Kit
Fig.1. Human Macrophage Migration Inhibitory Factor (MIF) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Macrophage migration inhibitory factor
Synonyms: Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase; MIF_HUMAN; A5Z1R8; B2R4S3; P14174; Q2V4Y5; Q6FHV0
Description:
MIF encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
MIF
GIF
GLIF
MMIF
macrophage migration inhibitory factor
phenylpyruvate tautomerase
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:26.89 pg/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human Macrophage Migration Inhibitory Factor microplate
• Human Macrophage Migration Inhibitory Factor standard
• Human Macrophage Migration Inhibitory Factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:37.5 - 2400pg/mL