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Human Bile salt export pump (ABCB11) ELISA Kit
Bile salt export pump
Synonyms: ATP-binding cassette sub-family B member 11; ABCBB_HUMAN; O95342; Q53TL2; Q9UNB2
ABCB11 is an ABC transporter named BSEP (Bile Salt Export Pump), or sPgp (sister of P-glycoprotein). This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).This protein is a member of the MDR/TAP subfamily. Some members of the MDR/TAP subfamily are involved in multidrug resistance. This particular protein is responsible for the transport of taurocholate and other cholate conjugates from hepatocytes (liver cells) to the bile. In humans, the activity of this transporter is the major determinant of bile formation and bile flow.
Test method:Colorimetric
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
ABC member 16
MDR/TAP subfamily
bile salt export pump
progressive familial intrahepatic cholestasis 2
sister p-glycoprotein
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:7.8 pg/mL
Sample type:
Cell culture supernatants
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human Bile salt export pump microplate
• Human Bile salt export pump standard
• Human Bile salt export pump detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:31.25 - 2000pg/mL