Liver Neoplasms
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Pubmed
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
March/14/2005
Abstract
OBJECTIVE
To investigate the relationship between the expression of mrp and both the responses to chemotherapy and the level of alpha-fetoprotein (AFP).
METHODS
S-P immunohistochemical staining and in situ PCR were adopted to detect MRP and mRNA mrp in 54 cancer tissues taken from untreated HCC patients whose tumor could not be removed during the operation, 24 para-cancer tissues, and 12 posthepatitis cirrhosis paraffin-embedded tissues. The relationship between the expression of mrp and their curative effect to chemotherapy in all the patients was analyzed, so was the relationship between the expression of mrp and the level of AFP in 38 patients whose AFP was positive after operation.
RESULTS
The positive rates of expressing MRP and mRNA mrp in the three kinds of tissues were 61.1%, 25.0%, 33.3% and 77.8%, 37.5%, 41.7%, respectively, with higher rates in HCC tissues than those in other tissues (chi2=9.842, P< 0.01; chi2=13.956, P<0.01). The rates of curative effect to chemotherapy in groups of negative and positive MRP and mRNA mrp expression were 61.9%, 30.3% and 75.0%, 33.3%, respectively, with significant difference between the negative and positive groups (chi2=5.242, P<0.05; chi2=6.627, P< 0.05). As the same as the percentage of curative effect to chemotherapy, the rates of AFP level decreased evidently were 62.5%, 27.3% and 87.5%, 30.0%, with remarkable difference between the two groups (chi2=4.710, P<0.05; chi2=8.566, P<0.05).
CONCLUSIONS
The multidrug resistance (MDR) of HCC is related to mrp expression, which initiates the intrinsic MDR. There is an important significance by detecting mrp expression in selecting chemotherapeutic method, and the expression of mrp can act as an indicator for chemotherapeutic sensitivity in HCC patients.
Pubmed
Journal: Ai zheng = Aizheng = Chinese journal of cancer
January/24/2005
Abstract
OBJECTIVE
It was reported that the hepatitis B virus X could inhibit the function of p53 and have contrary effects on p21(WAF1), a downstream gene of p53, but the mechanism is not clear up to now. So this study was designed to investigate HBx's effect on p21(WAF1) gene.
METHODS
After co-transfection of sense or antisense wild type p53 gene (wtp53) with HBx into SMMC-7721 and HBx alone transfection into other hepatoma cell lines with different endogenous status of p53, we evaluated the luciferase expression level under the p21(WAF1) promoter by detecting the luciferase activity and alteration of cell cycle in these transfected cell lines by flow cytometry. Expressions of p53 and p21 (WAF1) in hepatoma cells after transfected with HBx were also estimated by Western blot analysis.
RESULTS
The luciferase activity (1.007+/-0.098) in SMMC-7721 cells cotransfected with HBx and sense-wtp53 was higher than that in cells with wtp53 gene alone (0.490+/-0.012, P< 0.05), and also was depressed in the other experimental groups transfected with HBx (P< 0.05). Western blot analysis showed that after transfected with HBx, p53 expression was elevated in all hepatoma cell lines with different endogenous status of p53, and the expression of p21(WAF1) and luciferase activity under the p21(WAF1) promoter (0.053+/-0.010 vs. 0.094+/-0.013, P< 0.05) were both decreased in SMMC-7721 cells with low expression of p53, but relatively increased in HepG2 cell line with high expression of p53 (1.252+/-0.052 vs. 0.767+/-0.031, P< 0.05). Flow cytometry showed that fewer SMMC-7721 (42.31%) and Hep3B (36.96%) cells were arrested in G(0)/G(1) phase in transiently transfected HBx groups than in the control groups (47.10% and 42.90%), which was the opposite case in HepG2 cell line (63.62% vs. 57.42%). Moreover, after stably transfected with pcDNA3HBx, HepG2 cells reduced in G(0)/G(1) phase in compared with the control group (57.31% vs 61.49%).
CONCLUSIONS
HBx may not only increase the expression of p21(WAF1) by introducing the accumulation of p53 in cytoplasm but also inhibit the transcriptional activity of p21(WAF1) promoter in a p53-independent manner.
Pubmed
Journal: Surgery today
January/18/2011
Abstract
A 52-year-old woman presented with upper abdominal pain. Abdominal ultrasonography showed a 4-cm well-defined mass containing solid and cystic components in segment IV of the liver, and contrast-enhanced T1-weighted magnetic resonance imaging revealed heterogeneous enhancement within the tumor, indicating a solid or fibrous component. There were no cystic lesions in any other organs. A partial hepatectomy was performed, based on a preoperative diagnosis of sclerosing hemangioma and biliary cystadenoma or cystadenocarcinoma. Pathologically, the tumor appeared to be a multilocular and cystic lesion lined by attenuated endothelial- like cells with no atypia. Immunohistochemistry demonstrated the endothelial-like cells to be positive for the lymphatic-specific markers D2-40, LYVE-1, and Prox-1, which proved helpful for confirming the diagnosis as solitary hepatic lymphangioma. This case is presented with details of the pathologic and radiologic findings, because solitary hepatic lymphangioma is an extremely rare tumor and no previous reports have provided details of the immunohistochemical characteristics.
Pubmed
Journal: Acta radiologica: diagnosis
November/30/1996
Pubmed
Journal: Voprosy onkologii
May/28/2014
Abstract
There was held micro- and ultrastructural study of the liver of the Wistar rats in the dynamics of development of transplantable Walker 256 carcinosarcoma. The regularities of the progression of the tumor process in the form of liver metastases with appearance of intralobular metastases. In the early stages of tumor development it was marked activation of cytotoxic function of the liver with necrosis of tumor lesions accompanied by a decrease of the structural density of metastases. In advanced stages of development of the Walker 256 carcinosarcoma an estimation of parameters of tumor invasion in the liver showed an intensification of these processes with increased severity of inflammatory reactions.
Pubmed
Journal: ACS applied materials & interfaces
October/4/2016
Abstract
Although computer simulation and cell culture experiments have shown that elongated spherical particles can be taken up into cells more efficiently than spherical particles, experimental investigation on effects of these different shapes over the particle-membrane association has never been reported. Therefore, whether the higher cellular uptake of an elongated spherical particles is a result of a better particle-membrane association as suggested by some calculation works or a consequence of its influence on other cellular trans-membrane components involved in particle translocation process, cannot be concluded. Here, we study the effect of particle shape on the particle-membrane interaction by monitoring the association between particles of various shapes and lipid bilayer membrane of artificial cell-sized liposomes. Among the three shaped lanthanide-doped NaYF4 particles, all with high shape purity and uniformity, similar crystal phase, and surface chemistry, the elongated spherical particle shows the highest level of membrane association, followed by the spherical particle with a similar radius, and the hexagonal prism-shaped particle, respectively. The free energy of membrane curvature calculated based on a membrane indentation induced by a particle association indicates that among the three particle shapes, the elongated spherical particle give the most stable membrane curvature. The elongated spherical particles show the highest cellular uptake into cytosol of human melanoma (A-375) and human liver carcinoma (HepG2) cells when observed through a confocal laser scanning fluorescence microscope. Quantitative study using flow cytometry also gives the same result. The elongated spherical particles also possess the highest cytotoxicity in A-375 and normal skin (WI-38) cell lines, comparing to the other two shaped particles.
Pubmed
Journal: Lijecnicki vjesnik
November/30/1996
Pubmed
Journal: The Japanese journal of surgery
February/21/1982
Abstract
In cases of metastatic cancer of the liver in rats, the effect of ligation of the hepatic artery (HAL) or portal vein (PVL) with or without intraportal chemotherapy with Mitomycin C (MMC) was studied in Experiment I. HAL or PVL alone and chemotherapy alone induced only a slight anti-cancer effect. The most striking effect was found in the group treated with HAL plus MMC. PVL plus MMC produced intermediate results. To evaluate which route, via the hepatic artery or portal vein, is more effective for the adjuvant chemotherapy after HAL, Experiment II was conducted on the rats with a minute and/or a rather advanced metastatic cancer of the liver. Chemotherapy with MMC by either route alone and HAL alone did not substantially prolong the survival time, irrespective of the tumor size. HAL plus chemotherapy via both routes produced a statistically longer survival time compared with the control, however, better results were obtained by intraarterial chemotherapy. When compared with the two groups treated by the same procedures but at the different times, the earlier treatments produced better results. In the light of the present studies, it can be concluded that if cytocidal drugs with dose independent effects are given in a single bolus, HAL in connection with intra-arterial chemotherapy is a beneficial modality for the treatment of nonresectable liver cancer.
Pubmed
Journal: Journal d'urologie
February/22/1984
Abstract
The authors analyse a series of 186 patients with renal cell carcinoma in which 33 patients of Stage IV, with metastases, underwent Nephrectomy and 3 had also operative removal of metastases appearing in early post nephrectomy follow up. The survival periods have been disappointing (none at 5 years). With a review of literature they discuss about the extent of nephrectomy, whether done with purely palliative aim, or along with attempted curative excision simultaneously of the associated metastases. Even if the spontaneous regressions are exceptional and the improvement of survival duration less frequent, metastases are not always a reason not to remove Renal Cell Carcinoma.
Pubmed
Journal: Zeitschrift fur Gastroenterologie
March/23/2005
Abstract
Radiofrequency ablation (RFA) is currently the most established technique for the local destruction of tumors within the liver. It is technically straightforward, can be repeated and combines a high efficiency in tissue destruction with a low to moderate incidence of complications. This review explains the principle of RFA and lists currently available systems. In addition, the rationale behind RFA is discussed, and an overview of clinical indications, results and complications is presented. One of the main problems in assessing the clinical value of RFA is the fact that no large and controlled randomized studies are available, while the number of ablations and treated tumor entities are steadily increasing. From a technical point of view, the major problem is the still limited volume of necrosis that is achievable. The indication for RFA has to be discussed as interdisciplinary. A complete tumor necrosis should be the aim of all ablations, with the exception of symptomatic metastases of neuroendocrine tumors. The value of additional chemotherapy to RFA is still unclear, especially in the treatment of liver metastases.
Pubmed
Journal: Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca
November/30/1996
Pubmed
Journal: Antibiotiki
March/8/1967
Pubmed
Journal: Photomedicine and laser surgery
March/22/2017
Abstract
OBJECTIVE
This study evaluates the participation of immunological mechanisms of downregulation of murine hepatoma cells MH22a after direct exposure to polychromatic polarized light.
BACKGROUND
Previous studies have shown that exposure to a combination of visible (VIS) and infrared (IR) light leads to decreased tumorigenicity of the murine hepatoma cells MH22a, which correlated with an increase in the amount of cells with reorganized cytoskeleton in the submembrane region. The mechanism of tumor inhibition and elimination has not been determined.
METHODS
Polychromatic light (480-3400 nm) has been used at doses of 4.8 and 9.6 J/cm(2) to determine the sensitivity of murine MH22a cells and human erythroleukemia cells K562 exposed to this light, to lysis by effector cells of innate immunity (NK cells), and enhancement of the glycocalyx of the studied tumor cells. This was determined using flow cytometry, the H(3)-uridine cytotoxic test followed by spectrophotometry.
RESULTS
VIS-IR light increases the sensitivity of MH-22a cells at a dose 4.8 J/cm(2) and K562 cells at 9.6 J/cm(2). The enhancement of sensitivity of tumor cells to NK lysis changed their ability to absorb alcian blue, reflecting a change in the expression of the glycocalyx.
CONCLUSIONS
Increasing the sensitivity of the murine tumor cells MH22a and human K562 irradiated VIS-IR light correlated with a change in the expression of their glycocalyx. The results of the present study demonstrate that the reduction of tumorigenicity of irradiated tumor cells is due to their sensitivity to lysis by NK cells of the immune system.
Pubmed
Journal: The British journal of surgery
September/21/1997
Abstract
BACKGROUND
Liver resection for metastatic colorectal cancer has been established. Nevertheless, it is still controversial whether the surgical margin from the tumour edge to the cut surface of the liver is a significant prognostic factor in hepatic resection for colorectal metastatic liver cancer.
METHODS
To clarify the prognostic risk factors in hepatic resection for colorectal metastasis, univariate and multivariate analyses were performed. Between April 1985 and April 1995, 31 patients underwent curative hepatic resection for metastatic colorectal cancer. The clinical and pathological factors were examined retrospectively.
RESULTS
Overall 1-, 3- and 5-year survival rates of the patients were 92, 42 and 39 per cent respectively. Pathological study of 16 resected specimens with a solitary liver tumour revealed hepatic vein invasion by cancer cells in two of 16 cases, portal vein invasion in three, microsatellite lesions in two and biliary tract invasion in six cases. In resected specimens with a solitary tumour measuring less than 4 cm in diameter, one of these factors was observed in only two of nine cases, whereas in specimens with a solitary tumour measuring more than 4 cm in diameter, these factors were observed in six of seven patients (P < 0.05). The distance from the tumour edge to the intrahepatic invasion was less than 10 mm. With univariate analysis, tumour size of 4 cm or more in diameter, an interval of 6 months or less between colorectal and hepatic resection, four or more gross tumours, bilobar involvement and a resection margin from the tumour of less than 10 mm were found to be significant factors indicating a poor prognosis. Cox's proportional hazards model identified a tumour of 4 cm or more in diameter and a resection margin from the tumour of less than 10 mm as poor prognostic factors (P < 0.05).
CONCLUSIONS
In treating metastatic colorectal cancer to the liver, the surgical margin should be more than 10 mm because occult intrahepatic invasion was always found to be located within 10 mm from the edge of the tumour.
Pubmed
Journal: Cancer biotherapy & radiopharmaceuticals
September/18/2016
Abstract
To assess the prognostic role of total lesion glycolysis (TLG) in patients with breast cancer liver metastases (BCLM) after sequential lobar (90)Y-radioembolization ((90)Y-RE). Seventeen patients with bilobar BCLM underwent FDG PET/CT and TLG calculation before (90)Y-RE. The hepatic lobe with the highest TLG was treated in the first session. PET was performed 6 weeks postprocedure and decrease in TLG (ΔTLG) in the treated lobe was calculated before the second (90)Y administration. Subjects were divided in two groups (group 1: ΔTLG >50%, group 2: ΔTLG <50%). After the two consecutive (90)Y-therapies, patients underwent follow-up until death. Statistical analysis was performed to identify prognostic factors on overall survival (OS). After the first (90)Y administration, 10 cases showed a ΔTLG >50% and seven had a ΔTLG value <50%. After the two consecutive procedures, the mean OS for all patients was 13.5 ± 0.8 months. Subjects with a ΔTLG >50% and ΔTLG <50% had a mean OS of 16.4 ± 0.6 and 10.3 ± 0.4 months, respectively (p < 0.001). Cox regression analysis demonstrated hepatic tumor load (p = 0.048) and ΔTLG as the only significant (p = 0.005) predictors of survival. ΔTLG after the first (90)Y administration agrees with final outcome in BCLM patients after separate sequential lobar (90)Y-RE.
Pubmed
Journal: Bulletin - Office international des epizooties
August/3/1967
Pubmed
Journal: Archivum immunologiae et therapiae experimentalis
March/29/1967
Pubmed
Journal: Acta radiologica: diagnosis
April/26/1967
Pubmed
Journal: Gan
December/15/1967
Authors
Pubmed
Journal: Journal of biochemistry
November/30/1996
Pubmed
Journal: Medicinski glasnik
November/30/1996
Pubmed
Journal: Voprosy onkologii
June/3/1974
Pubmed
Journal: Nuntius radiologicus
December/12/1970
Pubmed
Journal: Biulleten' eksperimental'noi biologii i meditsiny
January/8/1971
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