Li Wang
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Publication
Journal: Pest management science
October/17/2019
Abstract
Imidacloprid (IMI) is a major neonicotinoid insecticide used to control Aphis gossypii Glover. However, resistance to IMI developed rapidly in A. gossypii. The feeding behaviour and life history changes associated with IMI resistance were studied in A. gossypii.

RESULTS
The resistant population with a point mutation (R81T) in the nAChR β1 subunit showed an IMI resistance ratio of 58.12. This IMI-resistant A. gossypii became more active in finding an appropriate position for feeding. They made more intercellular apoplastic stylet pathway events (C) than the susceptible population. Moreover, the probing and feeding behaviour of two aphid populations were dramatically altered by IMI. The phloem ingestion (E2) duration was significantly longer for IMI-resistant aphids on IMI-treated plants (WDI: 208.70±17.38 min) than on control plants (WDI: 133.80±16.37 min). However, IMI statistically reduced the ability of susceptible aphids to find and feed from the phloem. The number and duration of phloem-related activities were sharply decreased for the susceptible aphids treated with IMI. In addition, the resistant population showed an increased relative fitness of 1.36. The fecundity of IMI-resistant adults was dramatically higher than that of the susceptible population. This difference also led to an increase in the net reproductive rate (R0 ) for the IMI-resistant A. gossypii.

IMI provoked phloem-feeding more rapidly and effectively in IMI-resistant A. gossypii, but significantly suppressed the feeding of susceptible A. gossypii. Therefore, the resistance to IMI can result in stimulated feeding and fecundity and subsequent population outbreaks, which make the control of IMI-resistant A. gossypii more challenging. This article is protected by copyright. All rights reserved.
Publication
Journal: Leukemia
October/15/2019
Publication
Journal: Sensors (Basel, Switzerland)
September/24/2019
Abstract
To autonomously move and operate objects in cluttered indoor environments, a service robot requires the ability of 3D scene perception. Though 3D object detection can provide an object-level environmental description to fill this gap, a robot always encounters incomplete object observation, recurring detections of the same object, error in detection, or intersection between objects when conducting detection continuously in a cluttered room. To solve these problems, we propose a two-stage 3D object detection algorithm which is to fuse multiple views of 3D object point clouds in the first stage and to eliminate unreasonable and intersection detections in the second stage. For each view, the robot performs a 2D object semantic segmentation and obtains 3D object point clouds. Then, an unsupervised segmentation method called Locally Convex Connected Patches (LCCP) is utilized to segment the object accurately from the background. Subsequently, the Manhattan Frame estimation is implemented to calculate the main orientation of the object and subsequently, the 3D object bounding box can be obtained. To deal with the detected objects in multiple views, we construct an object database and propose an object fusion criterion to maintain it automatically. Thus, the same object observed in multi-view is fused together and a more accurate bounding box can be calculated. Finally, we propose an object filtering approach based on prior knowledge to remove incorrect and intersecting objects in the object dataset. Experiments are carried out on both SceneNN dataset and a real indoor environment to verify the stability and accuracy of 3D semantic segmentation and bounding box detection of the object with multi-view fusion.
Publication
Journal: Human brain mapping
September/18/2019
Abstract
Studying cortical hemispheric asymmetries during the dynamic early postnatal stages in macaque monkeys (with close phylogenetic relationship to humans) would increase our limited understanding on the possible origins, developmental trajectories, and evolutional mechanisms of brain asymmetries in nonhuman primates, but remains a blind spot to the community. Via cortical surface-based morphometry, we comprehensively analyze hemispheric structural asymmetries in 134 longitudinal MRI scans from birth to 20 months of age from 32 healthy macaque monkeys. We reveal that most clusters of hemispheric asymmetries of cortical properties, such as surface area, cortical thickness, sulcal depth, and vertex positions, expand in the first 4 months of life, and evolve only moderately thereafter. Prominent hemispheric asymmetries are found at the inferior frontal gyrus, precentral gyrus, posterior temporal cortex, superior temporal gyrus (STG), superior temporal sulcus (STS), and cingulate cortex. Specifically, the left planum temporale and left STG consistently have larger area and thicker cortices than those on the right hemisphere, while the right STS, right cingulate cortex, and right anterior insula are consistently deeper than the left ones, partially consistent with the findings in human infants and adults. Our results thus provide a valuable reference in studying early brain development and evolution.
Publication
Journal: Canadian journal of psychiatry. Revue canadienne de psychiatrie
September/12/2019
Abstract

BACKGROUND
Post-traumatic stress disorder (PTSD) and depression are common mental disorders in individuals experiencing traumatic events. To date, few studies have studied the relationship between genetic basis and phenotypic heterogeneity of traumatized individuals. The present study examined the effects of four FKBP5 SNPs (rs1360780, rs3800373, rs9296158, and rs9470080) in four postdisaster groups (low symptom, predominantly depressive, predominantly PTSD, and combined PTSD-depression symptom groups) as identified by latent profile analysis.

METHODS
A total of 1,140 adults who experienced the 2008 Wenchuan earthquake participated in our study. Earthquake-related trauma, PTSD, and depressive symptoms were measured using standard psychometric instruments. The four FKBP5 SNPs were genotyped using a custom-by-design 2 × 48-Plex SNP scan™ Kit.

After adjusting for covariates, the main and gene-environment interaction effects of rs9470080 were all significant when the combined PTSD-depression group was compared with the low symptoms, predominantly depression and predominantly PTSD groups. rs9470080 TT genotype carriers had a higher risk of developing high co-occurring PTSD and depression symptoms than the C allele carriers. However, when trauma exposure was severe, the TT genotype carriers and C allele carriers did not differ in the risk of developing high co-occurring PTSD and depressive symptoms. The other three SNPs demonstrated no significant effects. Moreover, the rs3800373-rs9296158-rs1360780-rs9470080 haplotype A-G-C-T was found significantly associated with combined PTSD-depression symptoms.

CONCLUSION
Our findings support the genetic basis of phenotypic heterogeneity in people exposed to trauma. Furthermore, the results reveal the possibility that the variants of FKBP5 gene may be associated with depression-PTSD comorbidity.

Publication
Journal: World neurosurgery
September/7/2019
Publication
Journal: Environmental pollution (Barking, Essex : 1987)
August/27/2019
Abstract
Propylea japonica is a very important predator in agricultural ecosystems, which could be exposed to Bt protein. In this study, the bacterial community of P. japonica fed with normal food and food containing Cry2Ab protein was characterized for the first time using qPCR and high-throughput sequencing approaches. Results showed no effect of Cry2Ab on P. japonica development and reproduction. The most abundant bacterial phylum was Firmicutes, and the most abundant genus was Staphylococcus. The total bacteria copy number was not significantly different across four larval stages. Bacteria species composition was gathered more closely in feed on sucrose solution (sucrose-fed) than in larvae only fed on pea aphid (aphid-fed), the diversity indices of some operational taxonomic unit (OTU) were significantly different between sucrose-fed and aphid-fed samples. Different instar larval stages of P. japonica fed with sucrose solution containing Cry2Ab Bt protein and found no effect on microbial community composition and total bacteria copy numbers. However, effects on relative abundance of microbes, copy numbers of Corynebacterium 1 and Glutamicibacter arilaitensis were observed significantly lower in Bt-fed first and fourth larval stages. Low and high concentrations of Cry2Ab protein altered the microbial abundance relative to sucrose-fed P. japonica and copy numbers of G. arilaitensis and Staphylococcus xylosus were significantly lower in Bt-fed samples than control sucrose-fed. Our results are the first report showing that feeding on Cry2Ab protein does not alter microbial species composition in P. japonica, but effects gene copy number of some dominant bacteria. Further investigations are needed to assess the effect of copy number change on P. japonica.
Publication
Journal: Cancer science
August/21/2019
Abstract
The underlying mechanisms of breast cancer cells metastasizing to distant sites are complex and multifactorial. Bone sialoprotein (BSP) and αvβ3 integrin were reported to promote the metastatic progress of breast cancer cells, particularly metastasis to bone. Most theories presume that BSP promotes breast cancer metastasis by binding to αvβ3 integrin. Interestingly, we found the αvβ3 integrin decreased in BSP silenced cells (BSPi), which have weak ability to form bone metastases. However, the relevance of their expression in primary tumor and the way they participate in metastasis are not clear. In this study, we evaluated the relationship between BSP, αvβ3 integrin levels, and the bone metastatic ability of breast cancer cells in patient tissues, and the data indicated that the αvβ3 integrin level is closely correlated to BSP level and metastatic potential. Overexpression of αvβ3 integrin in cancer cells could reverse the effect of BSPi in vitro and promote bone metastasis in a mouse model, whereas knockdown of αvβ3 integrin have effects just like BSPi. Moreover, The Cancer Genome Atlas data and RT-PCR analysis have also shown that SPP1, KCNK2, and PTK2B might be involved in this process. Thus, we propose that αvβ3 integrin is one of the downstream factors regulated by BSP in the breast cancer-bone metastatic cascade.
Publication
Journal: Applied microbiology and biotechnology
July/5/2019
Abstract
Thermostability plays an important role in the application of L-asparaginase in the pharmaceutical and food industries. Understanding the key residues and structures that influence thermostability in L-asparaginase is necessary to obtain suitable L-asparaginase candidates. In this study, special residues and structures that altered thermostability in thermophilic L-asparaginase and non-thermophilic L-asparaginase II were identified. Interchanging these special residues and structures of L-asparaginases from the four strains, that is, Pyrococcus yayanosii CH1 (PYA), Thermococcus gammatolerans (TGA), Bacillus subtilis (BSA II), and Escherichia coli (ECA II), revealed the 51st and 298th residues of PYA (corresponding to 57th, 305th residues of ECA II) as the key residues responsible for thermal stability of thermophilic L-asparaginase and non-thermophilic L-asparaginase II. Moreover, the C terminal tightness, loop rigidity, and low surface charge around activity sites were of great significance to the thermostability of L-asparaginase. This study therefore revealed the crucial amino acid residues and structures responsible for the difference in thermostability of the thermophilic and non-thermophilic L-asparaginase and provides a reference for engineering thermostability in L-asparaginase II.
Publication
Journal: Journal of medicinal chemistry
June/28/2019
Abstract
Starting from our previously developed c-KIT kinase inhibitor CHMFL-KIT-8140, through a type II kinase inhibitor binding element hybrid design approach, we discovered a novel c-KIT kinase inhibitor compound 18 (CHMFL-KIT-64), which is potent against c-KIT wt and a broad spectrum of drug-resistant mutants with improved bioavailability. 18 exhibits single-digit nM potency against c-KIT kinase and c-KIT T670I mutants in the biochemical assay and displays great potencies against most of the gain-of-function mutations in the juxtamembrane domain, drug-resistant mutations in the ATP binding pocket (except V654A), and activation loops (except D816V). In addition, 18 exhibits a good in vivo pharmacokinetic (PK) profile in different species including mice, rats, and dogs. It also displays good in vivo antitumor efficacy in the c-KIT T670I, D820G, and Y823D mutant-mediated mice models as well as in the c-KIT wt patient primary cells which are known to be imatinib-resistant. The potent activity against a broad spectrum of clinically important c-KIT mutants combining the good in vivo PK/pharmacodynamic properties of 18 indicates that it might be a new potential therapeutic candidate for gastrointestinal stromal tumors.
Publication
Journal: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
June/13/2019
Abstract
Currently, radiography with measurement of the Cobb angle is still considered the reference standard for diagnosing scoliosis. However, the ionizing radiation hazard is drawing wide attention. Can 3-dimensional (3D) ultrasound (US) be an alternative modality for diagnosing and monitoring patients with scoliosis? The aim of our study was to assess the reliability and validity of 3D US imaging in the evaluation of scoliosis.A commercially available ultrasound system with a magnetic tracking system was selected for long-distance 3D US imaging. Straight phantoms and curved phantoms were scanned with the imaging system to evaluate the stability of the system for curvature measurements. Eight healthy adult volunteers and 28 patients with scoliosis were recruited for long-distance 3D US imaging. The intraclass correlation coefficient was used to test the reproducibility of the interobserver and intraobserver measurements for both the healthy adults and patients with scoliosis. A linear regression analysis and Bland-Altman plot were used to analyze the correlation and to determine the extent of agreement between the angles measured on US images and the Cobb angles measured on conventional radiographs.

RESULTS
The 28 patients with scoliosis included 10 male and 18 female patients aged 8 to 37 years (mean age ± SD, 17.7 ± 1.4 years; body mass index, <25 kg/m2 ). In the phantom study, there was no statistically significant difference between the angles measured by the 3D US imaging system and those measured by an angle gauge (P > 0.05). In the clinical study, there was very good interobserver and intraobserver reliability (intraclass correlation coefficients, >0.90) for the US imaging system, with a high correlation (r2 = 0.92) and agreement between the US and radiographic methods.

The long-distance 3D US imaging system offers a viable modality for diagnosing and monitoring scoliosis without radiation.
Publication
Journal: Annals of transplantation
April/3/2019
Abstract
BACKGROUND Post-transplant lymphoproliferative disorder (PTLD) is a rare complication following solid organ transplantation and allogeneic hematopoietic stem cell transplantation (Allo-HSCT), which gives rise to high mortality rates. MATERIAL AND METHODS This was a single-center retrospective analysis based on 27 patients who were diagnosed with PTLD following Allo-HSCT between January 1, 2007 and June 2018 at the Chinese PLA General Hospital. The purpose of this analysis was to investigate responses and prognostic factors of rituximab-based treatment. RESULTS Twenty-seven patients were treated with rituximab. Among them, 20 of 27 patients (74.07%) had a complete response, 2 of 27 patients (7.41%) had a partial response, 5 of 27 patients (18.52%) had no response, and 22 of 27 patients (81.48%) cleared Epstein-Barr virus (EBV) copies. There were no obvious side effects. The 1-year overall survival (OS) estimate was 46.8% (95% CI, 23.1-65.5%). Univariate analysis revealed that lower OS was correlated with Eastern Cooperative Oncology Group (ECOG) score standard (3-4), Epstein-Barr virus (EBV) viral load (≥10⁶ copies/mL), bacteria or fungal infection, and EBV reactivation were positive after treatment with 1 or 2 doses of rituximab (P<0.05). Multivariate analysis showed that each of the following were independently associated with lower OS (P<0.05): female, ECOG score standard (3-4), and EBV reactivation were positive after treatment with 1 or 2 doses of rituximab. CONCLUSIONS Our results demonstrated that rituximab-based treatment was a safe and effective strategy for patients who were diagnosed with PTLD following Allo-HSCT. The identified prognostic factors may help to detect which PTLD patients are at a higher risk of mortality.
Publication
Journal: Physical review. E
February/20/2019
Abstract
This paper analyzes the forces on a cylindrical straight rod penetrated into a granular bed in the partially fluidized state. As gas flow is blown from the bed bottom, the resistance force decreases linearly with increasing gas flow velocity. After the bed is fluidized, the resistance force is close to zero. When the bed is under the action of vibration, the average resistance force (F_{b,ave}) linearly decreases with increasing vibration acceleration but is barely influenced by the vibration frequency. The bed cannot be completely liquefied under vibration only, and F_{b,ave} constantly exists with increasing vibration acceleration. Based on the equivalent effects of gas flow and vibration, the calculation formula of the resistance force in the partially fluidized bed is established as F_{b,ave}/F_{b,0}=1-u/u_{MF}-0.748Γ, and the minimum vibration liquid velocity is estimated as u_{ML}/u_{MF}=1-0.748Γ.
Publication
Journal: Journal of cataract and refractive surgery
February/1/2019
Publication
Journal: Ophthalmology
January/3/2019
Abstract
To investigate the difference between the segmented axial length (AL) and the displayed AL on an optical low-coherence reflectometry (OLCR) biometer and to compare the refractive prediction errors calculated using the segmented and displayed ALs.Retrospective case series.Four thousand nine hundred ninety-two eyes from 4992 patients in the theoretical study and 1758 eyes from 1758 patients in the refractive prediction error comparison.First, we calculated the segmented AL as the sum of geometrical ocular segments converted from the optical path length (OPL) in each medium. To convert the OPL to a geometrical distance in each medium, we used 4 sets of group refractive indices. Then, the mean absolute prediction error (MAE) was calculated with the displayed AL and segmented AL using 6 intraocular lens power formulas: Olsen, Barrett Universal II (Barrett), Haigis, Hoffer Q, Holladay 1, and Sanders-Retzlaff-Kraff trial (SRK/T).Segmented AL, difference in AL (segmented AL minus displayed AL), MAE, and percentage of eyes within 0.5 diopter (D) of error.The segmented ALs were up to 0.29 mm longer in short eyes and 0.50 mm shorter in long eyes. The differences in ALs were correlated negatively with the displayed ALs (r values, -0.941 to -0.913; P < 0.001). The MAEs were significantly lower using segmented ALs for all formulas except the Olsen in both the entire group and the long eye subgroup (AL, ≥26 mm) and for the Holladay 1 and Hoffer Q in the short eye subgroup (AL, ≤ 22 mm). Use of segmented ALs produced a greater percentage of eyes within 0.5 D of error for all formulas except the Olsen and Haigis for the entire group, for long eyes, and for the Holladay 1 in short eyes.The segmented ALs were longer in short eyes and shorter in long eyes compared with the displayed ALs calculated with a single group refractive index for the entire eye. The refractive accuracy with segmented ALs was improved in short eyes with the Hoffer Q and Holladay 1 formulas and in long eyes with all formulas except the Olsen formula.
Publication
Journal: Journal of colloid and interface science
November/29/2018
Abstract
Direct methanol fuel cells (DMFCs) are promising power sources for automobiles and portable electronic devices. Its commercialization depends on the anodes with high activity, low Pt content, and especially high stability towards methanol oxidation. Herein, a self-supported Pt nanoflakes and amorphous Ni(OH)2 on nickel foam composite electrode (Pt-doped Ni(OH)2, Pt content: 1.5 wt%) with rich defects was fabricated via a facile and low cost galvanic deposition method. This composite anode exhibits enhanced activity and stability for methanol oxidation in alkaline media, which mainly come from the synergistic effects between Pt nanoflakes and amorphous Ni(OH)2 on Ni foam substrate and defect engineering. During a typical methanol oxidation process over Pt-doped Ni(OH)2: Pt nanoflakes act as the active sites; amorphous Ni(OH)2 promotes the poison removal; Ni foam provides high electric conductivity and large area; defects sites contribute to the enhanced activity and stability. This work suggests that this self-supported and defect-enriched Pt-doped Ni(OH)2 composite catalyst is an alternative to commercial Pt-based electrocatalyst for low temperature DMFCs.
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Publication
Journal: Transfusion medicine reviews
November/27/2018
Abstract
Controversial results exist regarding the clinical benefits of single- vs double-unit umbilical cord blood transplantation (UCBT) in patients with hematologic diseases. A systematic review was conducted to evaluate this issue. The PubMed, Embase, and Cochrane Library databases were searched up to May 2018. A total of 25 studies including 6571 recipients were identified. Although double-unit UCB contained higher doses of total nucleated cells and CD34+ cells, it offered no advantages over single-unit UCB in terms of hematologic recovery, including the rate and speed of neutrophil and platelet engraftment. Double-unit UCBT was associated with higher incidences of grades II-IV acute and extensive chronic graft-vs-host disease, accompanied by a lower relapse incidence, which may be attributed to a graft-vs-graft effect induced by double-unit UCB. However, transplant-related mortality, disease-free survival, and overall survival were comparable between single- and double-unit UCBT. Although double-unit UCBT confers no clinical advantages over single-unit UCBT, certain patients, such as those at high risk of relapse, might benefit from double-unit UCBT, a possibility that needs to be clarified in future randomized trials.
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Publication
Journal: Intensive & critical care nursing
November/25/2018
Abstract
OBJECTIVE
The purpose of this study was to develop, validate and apply a ventilator-associated pneumonia prevention checklist in a single cardiac surgery centre.
METHODS
An initial checklist was designed based on the published care bundles for prevention of ventilator-associated pneumonia; the Delphi method used for validation. A total of 20 experts were invited to score the items and give suggestions for the checklist. The final checklist was then applied to patients receiving cardiac surgery. Non-compliance with the protocol and outcome indicators were observed.
RESULTS
Two rounds of Delphi were conducted. The final one-page checklist consisted of three main parts: (1) demographic data of the patient receiving cardiac surgery; (2) general assessment of the patient (3) checklist of prevention measures. The average time to complete the checklist was between two and four minutes. After the application of the checklist, the incidence of ventilator-associated pneumonia decreased from 14.48 to 5.47 episodes per thousand ventilator hours. In patients requiring >48 hours mechanical ventilation, the ventilator-associated pneumonia rate and duration of ventilation was significantly decreased.
CONCLUSIONS
A checklist was developed for ventilator associated pneumonia based on care bundles and validated using the Delphi method. The checklist appeared to be a useful tool in preventing ventilator associated pneumonia and shortening the ventilation time.
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Publication
Journal: Marine environmental research
November/21/2018
Abstract
Ocean acidification and warming is widely reported to affect the ability of marine bivalves to calcify, but little is known about the underlying mechanisms. In particular, the response of their calcifying fluid carbonate chemistry to changing seawater carbonate chemistry remains poorly understood. The present study deciphers sources of the dissolved inorganic carbon (DIC) in the calcifying fluid of the blue mussel (Mytilus edulis) reared at two pH (8.1 and 7.7) and temperature (16 and 22 °C) levels for five weeks. Stable carbon isotopic ratios of seawater DIC, mussel soft tissues and shells were measured to determine the relative contribution of seawater DIC and metabolically generated carbon to the internal calcifying DIC pool. At pH 8.1, the percentage of seawater DIC synthesized into shell carbonate decreases slightly from 83.8% to 80.3% as temperature increases from 16 to 22 °C. Under acidified conditions, estimates of percent seawater DIC incorporation decreases clearly to 65.6% at 16 °C and to 62.3% at 22 °C, respectively. These findings indicate that ongoing ocean acidification and warming may interfere with the calcification physiology of M. edulis through interfering with its ability to efficiently extract seawater DIC to the calcifying front.
Publication
Journal: Advanced materials (Deerfield Beach, Fla.)
November/18/2018
Abstract
Black phosphorus (BP), first synthesized in 1914 and rediscovered as a new member of the family of 2D materials in 2014, combines many extraordinary properties of graphene and transition-metal dichalcogenides, such as high charge-carrier mobility, and a tunable direct bandgap. In addition, it displays other distinguishing properties, e.g., ambipolar transport and highly anisotropic properties. The successful application of BP in electronic and optoelectronic devices has stimulated significant research interest in other allotropes and alloys of 2D phosphorene, a class of 2D materials consisting of elemental phosphorus. As an atomically thin sheet, the various interfaces presented in 2D phosphorene (substrate/phosphorene, electrode/phosphorene, dielectric/phosphorene, atmosphere/phosphorene) play dominant roles in its bottom-up synthesis, and determine several key characteristics for the devices, such as carrier injection, carrier transport, carrier concentration, and device stability. The rational design/engineering of interfaces provides an effective way to manipulate the growth of 2D phosphorene, and modulate its electronic and optoelectronic properties to realize high-performance multifunctional devices. Here, recent progress of the interface engineering of 2D phosphorene is highlighted, including the epitaxial growth of single-layer blue phosphorus on different substrates, surface functionalization of BP for high-performance complementary devices, and the investigation of the BP degradation mechanism in ambient air.
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Publication
Journal: Nature communications
November/15/2018
Abstract
Innate behaviors are genetically encoded, but their underlying molecular mechanisms remain largely unknown. Predator odor 2,4,5-trimethyl-3-thiazoline (TMT) and its potent analog 2-methyl-2-thiazoline (2MT) are believed to activate specific odorant receptors to elicit innate fear/defensive behaviors in naive mice. Here, we conduct a large-scale recessive genetics screen of ethylnitrosourea (ENU)-mutagenized mice. We find that loss of Trpa1, a pungency/irritancy receptor, diminishes TMT/2MT and snake skin-evoked innate fear/defensive responses. Accordingly, Trpa1 -/- mice fail to effectively activate known fear/stress brain centers upon 2MT exposure, despite their apparent ability to smell and learn to fear 2MT. Moreover, Trpa1 acts as a chemosensor for 2MT/TMT and Trpa1-expressing trigeminal ganglion neurons contribute critically to 2MT-evoked freezing. Our results indicate that Trpa1-mediated nociception plays a crucial role in predator odor-evoked innate fear/defensive behaviors. The work establishes the first forward genetics screen to uncover the molecular mechanism of innate fear, a basic emotion and evolutionarily conserved survival mechanism.
Publication
Journal: Mikrochimica acta
November/13/2018
Abstract
An electrochemiluminescence (ECL) based assay is described for the determination of the endocrine disruptor bisphenol A (BPA). The method is based on the use of carboxylated graphitic carbon nitride (C-g-C3N4) carrying an immobilized aptamer against BPA. In the presence of BPA, the ECL signal decreases due to ECL energy transfer from excited-state C-g-C3N4 to the BPA oxidation product. Under the optimal conditions, ECL intensity increases linearly in the 0.1 pM to 1 nM BPA concentration range. The detection limit is as low as 30 fM. The assay has excellent sensitivity, outstanding stability and high selectivity. It was applied to the determination of BPA in spiked water samples. Graphical abstract Aptamer modified carboxylated graphitic carbon nitride was synthesized and applied in an electrochemiluminescence-based aptasensor for bisphenol A.
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Publication
Journal: Diabetes therapy : research, treatment and education of diabetes and related disorders
November/13/2018
Abstract
BACKGROUND
The Joint Committee for Developing Chinese Guidelines (JCDCG) introduced the Chinese definition for metabolic syndrome (MS), which has been verified in southern Chinese people but not in northwestern Chinese people. We evaluated the MS definition proposed by the JCDCG in a northwestern Chinese population, in comparison with those of the revised National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATPIII) and the International Diabetes Federation (IDF).
METHODS
This population-based cross-sectional study was a part of the China National Diabetes and Metabolic Disorders Study conducted in Shaanxi province. We included 3243 participants aged ≥ 20 years. The age-adjusted MS prevalence was assessed per the 2007 Chinese population structure. The agreement between different definitions was assessed by the kappa statistic.
RESULTS
The standardized prevalence of JCDCG-MS, revised ATPIII-MS, and IDF-MS was 22.4%, 29.4%, and 24.9%, respectively. Among women, the agreement of the JCDCG definition with the revised ATPIII and the IDF definition was not good (κ = 0.599 and 0.601, respectively); 54.6% of the revised ATPIII-MS and 56% of the IDF-MS were defined as MS according to the JCDCG definition. Among men, the agreement of JCDCG definition with the revised ATPIII and IDF definitions was very good (κ = 0.863) and substantial (κ = 0.741), respectively.
CONCLUSIONS
The agreement of the JCDCG definition with the revised ATPIII and IDF definitions was insufficient in women. Compared with the other two definitions, the JCDCG definition underestimates MS prevalence in northwestern women.
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Publication
Journal: Nuclear receptor research
November/13/2018
Abstract
Small heterodimer partner (SHP, NR0B2) is identified as a unique orphan nuclear receptor that acts as a transcriptional repressor. SHP plays a crucial role in the control of various physiological processes and in several diseases by regulating the expression of disease-specific genes. Non-coding RNAs (ncRNAs), including long noncoding RNAs (lncRNAs) and microRNAs (miRNAs), are encoded of RNAs that are transcribed but not translated into proteins, which are involved in diverse developmental and cellular processes in eukaryotic organisms. Research during the past decade has identified factors participating in the regulation of ncRNAs biogenesis and function. In this review, we summarize recent findings demonstrating a critical role of SHP as a transcriptional regulator of ncRNAs expression and function.
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