Yan Li
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Pubmed
Journal: AIDS and behavior
September/21/2008
Abstract
Men who have sex with men (MSM) may account for an increasing proportion of China's HIV epidemic, but remain difficult to access for epidemiological studies due to high stigma. We compare the composition of two samples of MSM obtained in Guangzhou, China. The first survey, conducted in 2004, recruited MSM through convenience sampling. The second survey in 2006 used long-chain referral recruitment in the context of respondent-driven sampling. Compared to convenience sampling, the long-chain referral method included higher proportions of subgroups of MSM thought to be at elevated risk for HIV infection and more difficult to reach, including internal migrants and those engaging in commercial sex. Long-chain referral also recruited more MSM who were under 25 years, unemployed, and had lower education. We conclude that long-chain referral recruitment will be more effective in tracking the leading edge of the epidemic among MSM in China than convenience sampling.
Pubmed
Journal: Optics express
July/23/2009
Abstract
A fiber-optic pump-probe setup is used to demonstrate all-optical switching based on intersubband cross-absorption modulation in GaN/AlN quantum-well waveguides, with record low values of the required control pulse energy. In particular, a signal modulation depth of 10 dB is obtained with control pulse energies as small as 38 pJ. Such low power requirements for this class of materials are mainly ascribed to an optimized design of the waveguide structure. At the same time, the intersubband absorption fully recovers from the control-pulse-induced saturation on a picosecond time scale, so that these nonlinear waveguide devices are suitable for all-optical switching at bit rates of several hundred Gb/s.
Pubmed
Journal: Digestive diseases and sciences
May/16/2006
Abstract
The current animal models of esophagitis and Barrett's esophagus consist of surgeries that divert the gastroduodenal contents to the esophagus. The limitations of these models are the inability to control the amount and concentration of the refluxate and the causing of significant postoperative stress and morbidity. Eighteen adult rats were cannulated at the upper esophagus and connected to a subcutaneous osmotic micropump to perfuse the esophageal lumen with bile and acid. Animals were sacrificed after 7 days of perfusion. Histological changes were determined. Cell proliferation, apoptosis, lipid peroxidation, and glutathione were measured. Histopathological changes in the bile- or acid-perfused esophagus were consistent with the findings associated with reflux esophagitis. Enhanced proliferation and apoptosis were seen, along with increased oxidative stress. The external esophageal perfusion model enabled precise control of the injurious agent. It induced the histologic and cellular injury of reflux esophagitis after 7 days.
Pubmed
Journal: Hypertension (Dallas, Tex. : 1979)
June/19/2007
Abstract
The angiotensin-converting enzyme (ACE) I/D and the alpha-adducin (ADD1) Gly460Trp polymorphisms are associated with cardiovascular risk factors. In a prospective population study and in cell models, we investigated the combined effects of these 2 polymorphisms. We randomly recruited 1287 white subjects (women: 50.0%; mean age: 55.9 years). We obtained outcomes from registries and repeat examinations (median 3). Over 9.0 years (median), 178 fatal or nonfatal cardiovascular events occurred. In ADD1 Trp allele carriers, the multivariate-adjusted hazard ratios associated with ACE DD versus I were 1.72 (P=0.007) for total mortality, 2.35 (P=0.02) for cardiovascular mortality, 2.02 (P=0.005) for all cardiovascular events, and 2.59 (P=0.03) for heart failure. In contrast, these hazard ratios did not reach significance in ADD1 GlyGly homozygotes (0.08<or=P<or=0.90). The positive predictive value and attributable risk associated with ACE DD homozygosity combined with mutated ADD1 were 36.2% and 10.3%, respectively. To clarify our epidemiological observations, we investigated the effects of mutated human ADD1 on the membrane-bound ACE activity in fibroblasts from 51 volunteers and in transfected human embryonic kidney cells (31 experiments). In fibroblasts (5.10 versus 3.63 nanomoles of generated hippuric acid per milligram of protein per minute; P=0.0021) and human embryonic kidney cells (1.086 versus 0.081 nmol/mg per minute; P=0.017), the membrane-bound ACE activity increased in the presence but not absence of the ADD1 Trp allele. In conclusion, the combination of ACE DD homozygosity and mutated ADD1 worsened cardiovascular prognosis to a similar extent as classic risk factors, possibly because of increased membrane-bound ACE activity in subjects carrying the ADD1 Trp allele.
Pubmed
Journal: Journal of theoretical biology
December/21/2014
Abstract
Knowledge of protein structural classes plays an important role in understanding protein folding patterns. Prediction of protein structural class based solely on sequence data remains to be a challenging problem. In this study, we extract the long-range correlation information and linear correlation information from position-specific score matrix (PSSM). A total of 3600 features are extracted, then, 278 features are selected by a filter feature selection method based on 1189 dataset. To verify the performance of our method (named by LCC-PSSM), jackknife tests are performed on three widely used low similarity benchmark datasets. Comparison of our results with the existing methods shows that our method provides the favorable performance for protein structural class prediction. Stand-alone version of the proposed method (LCC-PSSM) is written in MATLAB language and it can be downloaded from http://bioinfo.zstu.edu.cn/LCC-PSSM/.
Pubmed
Journal: Hypertension research : official journal of the Japanese Society of Hypertension
July/24/2011
Abstract
The aim of this study was to investigate how frequent ambulatory blood pressure (ABP) readings need to be obtained to reproduce the ambulatory arterial stiffness index (AASI) and pulse pressure (PP) without loss of information. We compared concordance from full and reduced ABP recordings. We recorded 24-h ABP at 30-min intervals in 1542 residents of Ohasama, Japan (baseline age, 40-93 years; 63.4% women). We randomly excluded up to 16 readings per recording or we selected readings at fixed 1- or 2-h intervals. Using full recordings as reference, we computed for the reduced recordings repeatability coefficient by Bland and Altman's approach. By Cox regression, we also calculated multivariate-adjusted hazard ratios for cardiovascular mortality. The median number of ABP readings per recording was 46. Randomly excluding more readings reduced the concordance of AASI, but not PP. Selecting blood pressure readings at 1- or 2-h intervals produced mean values of AASI and PP, which significantly differed from those in full recordings. During follow-up (median, 13.3 years) 126 cardiovascular deaths occurred. Across quartiles, AASI significantly predicted cardiovascular mortality in a U-shaped manner. AASI lost its prognostic significance when the number of randomly excluded readings increased from 8 to 16 or when the interval between readings was 1 h or longer. Compared with PP, AASI is less reproducible when the number of readings in ABP decreases, but this does not affect the predictive accuracy of AASI for cardiovascular mortality, until the median number of readings per ABP recording is less than ∼35.
Pubmed
Journal: PloS one
November/7/2014
Abstract
RBR (RING1-IBR-RING2) proteins play an important role in protein ubiquitination and are involved in many cellular processes. Recent studies showed plant RBR genes were induced by abiotic and biotic stresses. However, detailed studies on RBR genes in the important oil crop, soybean (Glycine max (L.) Merr.), is still lacking. Here we performed a genome-wide search and identified 24 RBR domain-containing genes from the soybean genome sequence and cloned 11 of them. Most soybean RBR proteins contain a highly conserved RBR supra-domain. Phylogenetic analyses indicated all 24 soybean RBR proteins are most related to the RBR proteins from Phaseolus vulgaris, and could be classified into seven groups including Ariadne A, Ariadne B, ARA54, Plant IIA, Plant IIB, Plant IIC, and Helicase. Tandem duplication and block duplication were found among the Ariadne B and Plant IIC group of soybean RBR genes. Despite the conserved RBR supra-domain, there are extensive variations in the additional protein motifs and exon-intron structures between different groups, which indicate they might have diverse functions. Most soybean RBR proteins are predicted to localize in nucleus, and four of them were experimentally confirmed by GFP fusion proteins. Soybean RBR genes are broadly expressed in many tissue types with a little more abundant in the roots and flowers than leaves, stems, and seeds. The expression of GmRTRTP3 (Plant IIB) and GmRTRTP5 (Plant IIC) are induced by NaCl treatment, which suggests these RBR genes might be involved in soybean response to abiotic stresses.
Pubmed
Journal: Future oncology (London, England)
November/14/2018
Abstract
OBJECTIVE
We aimed to investigate the expression of voltage-gated potassium channels KCND1/KCND2/KCND3 in gastric cancer (GC) and normal stomach tissues and to investigate the prognostic value of the upregulated gene KCND2.
METHODS
A retrospective analysis was performed using data from large available databases.
RESULTS
KCND2 was significantly upregulated at the mRNA and protein levels in GC compared with that in normal stomach tissues. High KCND2 RNA expression was independently associated with shorter overall survival (HR: 1.634, 95% CI: 1.135-2.352; p = 0.008) and recurrence-free survival (HR: 2.644, 95% CI: 1.438-4.863; p = 0.002). Data mining in the Kaplan-Meier plotter confirmed the prognostic value of KCND2.
CONCLUSIONS
KCND2 upregulation is a valuable prognostic biomarker in GC patients, in terms of overall survival and recurrence-free survival.
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Pubmed
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
April/22/2016
Abstract
OBJECTIVE
To develop a novel immunosuppressant GPI-CTLAIg modified by glycosyl-phosphatidylinositol(GPI).
METHODS
GPI-modified CTLAIg was produced by linking-up of CTLA4Ig with GPI-modification signal sequences from decay-accelerating factor (DAF). Chimeric molecule GPI-CTLA4Ig gene was cloned into eukaryotic expression vector pCI-dhfr. Using lipofectine-mediated gene transfer technique, pCI-GPI-CTLA4Ig was transfected into CHO-dhfr(-) cells, and the transfectants were screened by methotrexate (MTX). Expression of the recombinant protein was assessed by RT-PCR, ELISA, cell immunofluorescence staining and Western blot, and the purification of expressed protein was performed by protein A affinity chromatography.
RESULTS
The chimeric molecule GPI-CTLA4Ig has been constructed and stablely expressed on CHO-dhfr(-) cells.
CONCLUSIONS
GPI-modified CTLAIg will may be used as novel immunosuppressant for suppressing reaction in graft rejection.
Pubmed
Journal: Biosensors & bioelectronics
February/6/2017
Abstract
A paper-based multi-anode microbial fuel cell (PMMFC) integrated with power management system (PMS) was developed as a disposable self-support real-time "shock" biosensor for wastewater. PMMFCs were examined at three types of shocks (chromium, hypochlorite and acetate) in a batch-mode chamber, and exhibited various responses to shock types and concentrations. The power output of PMMFC sensor was four times as the carbon cloth (CC)-based MFCs, indicating the advantage of paper-based anode for bacterial adhesion. The power output was more sensitive than the voltage output under shocks, and thus preventing the false signals. The simulation of power harvest using PMS indicated that PMMFC could accomplish more frequent data transmission than single-anode MFCs (PSMFC) and CC anode MFCs (CCMMFC), making the self-support wastewater monitor and data transmission possible. Compared with traditional MFC sensors, PMMFCs integrated with PMS exhibit the distinct advantages of tight paper-packed structure, short acclimation period, high power output, and high sensitivity to a wide range of shocks, posing a great potential as "disposable self-support shock sensor" for real time in situ monitoring of wastewater quality.
Pubmed
Journal: Leukemia research
July/16/2017
Abstract
Acquired loss of the X chromosome (-X) as a sole abnormality is detected rarely in bone marrow (BM) and its clinical importance remains largely unknown. We studied 38 patients with isolated -X in BM. All patients were women, with a median age of 71 years. At the time of -X detection, BM was positive for myeloid neoplasm in 14 patients, lymphoma/myeloma in 10 patients, and was normal in 14 patients. -X was detected as a major clone in 15 patients (11 of them had myeloid neoplasm) and a minor clone in 23 patients. Combined morphologic and FISH analysis was performed in 16 cases, -X was detected in myeloid/erythroid cells in all 16 patients and in lymphocytes in 15 patients. With a median of 23 months follow-up, none of the patients with a negative BM or BM with involvement by lymphoid neoplasms developed a secondary myeloid neoplasm. We conclude that isolated -X is a rare finding in BM. In majority of patients, -X presents as a minor clone and is likely to be an aging effect or a benign finding; whereas when -X presents as a major clone in BM, it is often disease associated.
Pubmed
Journal: American journal of respiratory cell and molecular biology
January/24/2013
Abstract
Multiple cells contribute to the function of lungs. Pulmonary neuroendocrine cells (PNECs) are important for the regulation of breathing and carcinogenesis, although they represent only a small population of the airway lining. Achaete-Scute homologue-1 (Ascl1), a proneural basic helix-loop-helix transcription factor, is critical for the development of PNECs. We postulated that Ascl1-defined cells (ASDCs) may be progenitors, and traced their fate during development and injury repair. R26R-stop-lacZ (Rosa) reporter mice were crossed with Ascl1-Cre or Ascl1-CreERTM mice, in which the Ascl1 promoter drives the expression of Cre or inducible Cre recombinase, respectively. ASDCs and their descendants will be permanently labeled. The labeled cells were characterized by immunohistochemistry, using highly specific differentiation markers. Lineage studies revealed a population that proliferates before the pseudoglandular stage, and widely contributes to different compartments. When ASDCs were labeled on Embryonic Day 9.5, they gave rise to both airway and alveolar cells, but when labeled on Embryonic Day 11.5, they only gave rise to airway cells. In postnatal naphthalene injury, ASDCs contributed to regenerating Clara cells. In conclusion, Ascl1-defined cells in the lung represent a novel multipotent lineage, indicating a close relationship of neuroendocrine cells with other cell types.
Pubmed
Journal: Chinese medical journal
March/7/2011
Pubmed
Journal: Journal of cellular physiology
October/11/2018
Abstract
Craniofacial defects can cause morbidness. Adipose-derived stem cells (ADSCs) have shown great promise for osteogeneration and vascularization; therefore cocultures of differentiated ADSCs are explored to increase bone and vessel formation. In this study, ADSCs were induced into osteogenic ADSCs (os-ADSCs) and endothelial ADSCs (endo-ADSCs) cells, which were then cocultured in variable proportions (os-ADSCs/endo-ADSCs = 2:1, 1:1, 1:2). The os-ADSCs in a ratio of 1:1 expressed more ALP, RUNX2 and COL-I, whereas VEGF, vWF and CD31 were upregulated in the endo-ADSCs of this group. Next generation RNA sequencing (RNA-seq) was performed to evaluate the molecular mechanisms of cocultured ADSCs. The os-ADSCs and endo-ADSCs interacted with each other during osteogenic and angiogenic differentiation, especially at the ratio of 1:1, and were regulated by vascular-related genes, cell-mediated genes, bone-related genes and the transforming growth factor β signaling pathway (TGF-β), mitogen-activated protein kinase signaling pathway (MAPK) and wnt signaling pathway (Wnt). Angptl4, apoe, mmp3, bmp6, mmp13 and fgf18 were detected to be up-regulated, and cxcl12 and wnt5a were down-regulated. The results showed that the gene expression levels were consistent with that in RNA-seq. The cells were then seeded into self-assembling peptide RADA16-I scaffolds as cocultures (1:1) and monocultures (ADSCs, os-ADSCs, endo-ADSCs). The results showed that the cells of all groups grew and proliferated well on the scaffolds, and the cocultured group exhibited better osteogeneration and vascularization. In conclusion, cocultured os-ADSCs and endo-ADSCs at the ratio of 1:1 showed strong osteogenic and angiogenic differentiation. There is a great potential for osteogenesis and vascularization by 3D culturing cells in a 1:1 ratio in self-assembling peptide RADA16-I scaffolds, which requires evaluation for bone regeneration in vivo.
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Pubmed
Journal: Biochemical and biophysical research communications
November/1/2015
Abstract
Trichostatin A (TSA) is a histone deacetylase inhibitor and a potential therapeutic for various malignancies. The in vivo effect of TSA, however, has not been investigated in a transgenic lung cancer model. Previously, we generated transgenic mice with overexpression of Groucho-related-gene 1 (Grg1) and these mice all developed mucinous lung adenocarcinoma. Grg1 is a transcriptional co-repressor protein, the function of which is thought to depend on HDAC activity. However, functions outside the nucleus have also been proposed. We tested the supposition that Grg1-induced tumorigenesis is HDAC-dependent by assaying the therapeutic effect of TSA in the Grg1 transgenic mouse model. We found that TSA significantly inhibited lung tumorigenesis in Grg1 transgenic mice (p < 0.01). TSA did not affect overall Grg1 protein levels, but instead reduced ErbB1 and ErbB2 expression, which are upregulated by Grg1 in the absence of TSA. We confirmed this effect in A549 cells. Furthermore, lapatinib, an inhibitor of both ErbB1 and ErbB2, effectively masked the effect of TSA on the inhibition of A549 cell proliferation and migration, suggesting TSA does work, at least in part, by downregulating ErbB receptors. We additionally found that TSA reduced the expression of VEGF and VEGFR2, but not basic FGF and FGFR1. Our findings indicate that TSA effectively inhibits Grg1-induced lung tumorigenesis through the down-regulation of ErbB1 and ErbB2, as well as reduced VEGF signaling. This suggests TSA and other HDAC inhibitors could have therapeutic value in the treatment of lung cancers with Grg1 overexpression.
Pubmed
Journal: Journal of environmental sciences (China)
August/28/2013
Abstract
The concentration of polychlorinated biphenyls (PCBs) in the urban air of Dalian, China was monitored from November 2009 to October 2010 with active high-volume sampler and semipermeable membrane device (SPMD) passive sampler. The concentration of PCBs (particle + gas) (SigmaPCBs) ranged from 18.6 to 91.0 pg/m3, with an average of 50.9 pg/m3, and the most abundant dioxin-like PCB (DL-PCBs) was PCB118. The WHO-TEQ values of DL-PCBs were 3.6-22.1 fg/m3, with an average of 8.5 fg/m3, and PCB126 was the maximum contributor to SigmaTEQ. There was a much larger amount of PCBs in the gas phase than in the particulate phase. The dominant PCB components were lower and middle molecular weight PCBs. With increasing chlorination level, the concentration of the PCB congeners in the air decreased. The gas-particulate partitioning of PCBs was different for the four seasons. The gas-particulate partitioning coefficients (logKp) vs. subcooled liquid vapor pressures (logP(L)0) of PCBs had reasonable correlations for different sampling sites and seasons. The absorption mechanism contributed more to the gas-particulate partitioning process than adsorption. Correlation analysis of meteorological parameters with the concentration of PCBs was conducted using SPSS packages. The ambient temperature and atmospheric pressure were important factors influencing the concentration of PCBs in the air. The distribution pattern of the congeners of PCBs and the dominant contributors to DL-PCBs and TEQ in active samples and SPMDs passive samples were similar. SPMD mainly sequestrated gas phase PCBs.
Pubmed
Journal: Annals of surgery
November/13/2011
Abstract
OBJECTIVE
Aggressive human melanomas express, C-X-C chemokine receptor 4 (CXCR4), the receptor for the chemokine, stromal cell-derived factor-1alpha (SDF-1α). The CXCR4-SDF-1α axis has been postulated to increase melanoma invasiveness. We discovered that SDF-1α specifically upregulates E-selectin on endothelial cells, thus tethering circulating endothelial progenitor cells (EPC) and facilitating homing. We investigated the hypothesis that small interfering ribonucleic acid (siRNA)-mediated E-selectin blockade inhibits melanoma angiogenesis and tumor growth.
METHODS
Human melanoma cells overexpressing SDF-1α were xenografted on severe combined immunodeficiency (SCID) mice. SDF-1α expression in cells was measured by enzyme-linked immunosorbent assay (ELISA). In vitro melanoma cell growth was examined by cell proliferation assay. In vivo vascular E-selectin knockdown was achieved by administration of high-volume E-selectin siRNA (100 pmol/180 μL/week × 3 times) and inhibition was validated by immunostaining (N = 6/group, E-Selectin siRNA vs control siRNA). Tumor angiogenesis was quantified (DiI-perfusion and LASER confocal microscopy). EPC homing to tumor vasculature was detected by immunostaining. Explanted in vivo tumor size and weight were measured.
RESULTS
Three melanoma cells tested expressed undetectable levels of SDF-1α. Additional enforced overexpression of SDF-1α (by Lenti-SDF-1α) increased melanoma cell growth both in vitro and in vivo, enhanced EPC homing to tumor tissue, and increased tumor angiogenesis. Knocking-down vascular E-selectin significantly inhibited SDF-1α-induced EPC homing, tumor angiogenesis, and decreased melanoma growth in vivo.
CONCLUSIONS
Downregulation of vascular E-selectin profoundly inhibits EPC homing, tumor angiogenesis, and tumor growth in human melanoma xenograft murine model, potentially by suppression of E-selectin-mediated EPC-endothelial cells interactions/homing. These findings identify E-selectin as a novel target for inhibition of melanoma angiogenesis and tumor growth.
Pubmed
Journal: The Journal of infectious diseases
June/5/2006
Pubmed
Journal: Journal of natural products
September/20/2012
Abstract
Laxiflorolides A (1) and B (2), two unprecedented epimeric bishomoditerpene lactones with a unique C(22) framework, along with laxiflorins P-R (3-5), maoecrystal P (6), maoecrystal C (7), and eriocalyxin B (8), were isolated from the leaves of I. eriocalyx var. laxiflora. The structures of 1 and 2, including the absolute configurations, were determined by spectroscopic methods and single-crystal X-ray diffraction analysis. All of the compounds isolated were evaluated for their cytotoxicity against five tumor cell lines. Compounds 3, 6, and 8 showed remarkable cytotoxic activity against certain cell lines compared with the positive control.
Pubmed
Journal: Molecular biology reports
February/1/2010
Abstract
A suppression subtraction hybridization (SSH) cDNA library had been constructed to identify differentially expressed genes. Based on the sequence of an expressed sequence tag (EST) homologous to Pisum sativum zinc finger protein mRNA (Accession number: AF160911), the full-length cDNA of 1,676 nucleotides was cloned from alfalfa by rapid amplification of cDNA ends (RACE). It was designated as MsZFN, encoding a protein of 418 amino acids. The amino acid sequence compared by blast revealed high homology with zinc finger protein of other plants. Sequence comparison showed that there were five conserved typical zinc finger motifs, and one sugar transfer protein signature. The calculated molecular weight of the MsZFN protein was 45.8 k Da, and theoretical isoelectric point was 8.13. The MsZFN localized in nucleus. Under normal growth conditions, differential expression of MsZFN exhibited that the expression was the highest in leaf and the lowest in root. MsZFN was quickly and transiently induced by NaCl treatment and reached its maximum at 30 min.
Pubmed
Journal: Biomaterials
March/25/2014
Abstract
Cancer invasion and metastasis remains the root cause of mortality. This process involves alterations of tumor microenvironment, particularly the remodeling of extracellular matrix, characterized by collagen IV uncoiling, degradation, fragments deposition and cross-linking. Quantum dots-labeled molecular probes are promising platforms to simultaneously study several subtle changes of key biomolecules, because of their unique optical and chemical properties. Here we report on a quantum dots-based imaging technology to study key components in tumor microenvironment during cancer progression, so as to gain new insights into the role of collagen IV plays, to define the cancer "invasion unit" and to develop the "pulse-mode" of cancer invasion.
Pubmed
Journal: Medical science monitor : international medical journal of experimental and clinical research
August/4/2010
Abstract
BACKGROUND
The transendothelial migration of polymorphonuclear leukocytes (PMNs, neutrophils) may be a hallmark of acute lung injury (ALI). The breakdown of the vascular endothelial barrier has likewise been considered to have etiologic linkage in the pathogenesis of ALI. Rho-associated coiled-coil-forming protein kinase (ROCK), a downstream target effector of the small GTP-binding protein Rho, plays a key role in cell adhesion, motility, and contraction mediated by reorganization of the actin cytoskeleton. The aims were to investigate protection by fasudil in lipopolysaccharide (LPS)-induced ALI and the role of ROCK2 in neutrophil transendothelial migration in a murine model.
METHODS
Mice were assigned to three groups: sham-treated controls (Sham group), LPS instillation (LPS group), and protective application of fasudil and LPS instillation (Fasudil/LPS group). Indexes tested were breathing frequency, histopathological examination, lung injury score, lung wet-to-dry weight ratio, neutrophil percentage in bronchoalveolar lavage fluid (BALF), myeloperoxidase activity, and ROCK2 mRNA expression in lung homogenate.
RESULTS
Permeability pulmonary edema (histopathological examination, lung injury score, and lung wet-to-dry weight ratio) was ameliorated and neutrophil infiltration in the lungs (neutrophil percentage in BALF, myeloperoxidase activity) was depressed in response to fasudil administration. Expression of ROCK2 mRNA in the lung homogenates of the LPS-treated mice increased approximately fourfold; however, fasudil did not affect the increase.
CONCLUSIONS
The Rho/Rho kinase pathway may play an important role in the pathogenesis of LPS-induced ALI and fasudil, as a ROCK inhibitor, could decrease neutrophil transendothelial migration by attenuating cytoskeletal rearrangement of endothelial cells, leading to the inhibition of ALI development.
Pubmed
Journal: Gynecologic oncology
February/11/2008
Abstract
OBJECTIVE
E-cadherin plays an important role in the origin of epithelial ovarian cancer. However, the exact molecular mechanism by which this occurs is unknown. The polymorphisms located at the E-cadherin may contribute to an increased risk for certain cancers. In this paper, we studied the association between polymorphisms of E-cadherin and the risk of epithelial ovarian cancer.
METHODS
We assessed the -160C/A, -347G/GA polymorphism within the promoter region and 3'-UTR +54C/T polymorphism of E-cadherin in epithelial ovarian cancer and control women. We also tested the expression of E-cadherin protein in ovarian cancer tissue among three genotype (3'-UTR +54C/T polymorphism) carriers.
RESULTS
There was no significant difference in genotype distribution of the -160C/A and -347G/GA SNPs in the E-cadherin gene promoter region between ovarian cancer patients and controls, but haplotype -160A/-347GA relative to haplotype -160C/-347G was 48.6 (95% CI=2.9-806.2) for epithelial ovarian cancer risk. The C/C genotype of the 3'-UTR +54C/T polymorphism relative to the C/T+T/T genotype was 1.85 (95% CI=1.27-2.69) for epithelial ovarian cancer risk. E-cadherin protein expression in was lower in C/C genotype carriers than T allele carriers in ovarian cancer tissue (P=0.02).
CONCLUSIONS
The C/C genotype of 3'-UTR C/T SNP and -160C/-374GA haplotype in E-cadherin gene may be a potential susceptibility factor for risk of epithelial ovarian cancer in Chinese, which indicated that the lower expression of E-cadherin might play an important role in the pathogenesis of epithelial ovarian cancer.
Pubmed
Journal: The Science of the total environment
November/5/2006
Abstract
PM(2.5) samples were collected simultaneously at three representative areas (central city, industrial area and clean air suburban) of Shanghai City. Their morphologies and elemental compositions were determined by scanning electron microscopy coupled with energy analysis (SEM-EDX). The particles were classified into four groups based on morphology and elemental composition. Soot aggregates and spherical fly ash particles were the two dominant types and they were identified as originating from automobile exhaust, metallurgical industry and coal combustion. The size distribution of the particles showed that most had diameters in the range of 0.2-1.4 microm. Individual particles were measured by synchrotron radiation micro-beam X-ray fluorescence (micro-SXRF) and the micro-SXRF spectra were obtained. Pattern recognition techniques, which took the micro-SXRF spectrum of a single aerosol particle as its fingerprint, were used to identify the origins of the particles. Seven source types were identified. They were: metallurgical industry, vehicle exhaust, soil dust, coal combustion, diesel exhaust, oil combustion and motorcycle exhaust. Metallurgical industry, automobile exhaust, and coal combustion were recognized to be the main pollution sources of PM(2.5) in the air of Shanghai City.
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