Wei Chen
Sort
Pubmeds
(5,237)
Author
Wei Chen
Related with
Pubmeds(5243)
Affiliates(328)
Pubmed
Long non-coding RNA NEAT1 overexpression is associated with poor prognosis in cancer patients: a systematic review and meta-analysis.
Journal: Oncotarget
April/20/2017
Description

OBJECTIVE

Long non-coding RNAs (lncRNAs) are playing important roles in cancer progression and metastasis. Recent studies have demonstrated that the lncRNA, nuclear paraspeckle assembly transcript 1 (NEAT1), was aberrantly up-regulated in various types of cancers and was reported to be associated with unfavorable prognosis in cancer patients. This study examined the relationship between NEAT1 and relevant clinical outcomes.

RESULTS

A total of 1354 patients from 11 eligible studies were included in the meta-analysis. The results showed that high expression level of NEAT1 was significantly associated with shorter overall survival in cancer patients (hazard ratio (HR) = 1.53, 95% confidence interval (CI) = 1.36-1.71); in the subgroup analysis, the positive association was also found in patients with hepato-gastroenterol cancers (HR = 1.79, 95% CI = 1.48-2.16), non-small cell lung cancer (HR = 1.35, 95% CI = 1.04-1.76), ovarian cancer (HR = 1.41, 95% CI = 1.11-1.79) and other types of cancers (HR = 1.42, 95% CI = 1.11-1.81). The clinicopathological parameters analysis further showed that increased expression level of NEAT1 was positively correlated with larger tumor size (odds ratio (OR) = 1.74, 95% CI = 1.26-2.41), lymph node metastasis (OR = 2.29, 95% CI = 1.71-3.06), advanced TNM stage (OR = 3.60, 95% CI = 2.27-5.72), poor tumor differentiation (OR = 2.16, 95% CI = 1.58-2.93), distant metastasis (OR = 3.51, 95% CI = 1.75-7.01), and invasion depth (OR = 1.94, 95% CI = 1.36-2.75).

METHODS

A comprehensive search was performed in Pubmed, Embase, Web of Science and CNKI databases, and eligible studies were included based on defined exclusion and inclusion criteria to perform meta-analysis.

CONCLUSIONS

The meta-analysis results from present study suggested that increased expression level of NEAT1 was associated with unfavorable prognosis and may serve as a predictive factor for clinicopathological features in various cancers.

Read more
Authors
; ; ; ; ; ;
Related with
Citations(7)
References(34)
Affiliates(1)
Authors(7)
Pubmed
Influence of the metabolic syndrome versus the sum of its individual components on left ventricular geometry in young adults (from the Bogalusa Heart Study).
Journal: The American journal of cardiology
July/23/2009
Description

Current preventive cardiology guidelines strongly recommend identification of metabolic syndrome (MS), a constellation of cardiovascular risk factors, in clinical practice. These MS risk factors, individually or in a cluster, adversely alter left ventricular (LV) geometry. However, it is still unclear whether MS predicts risk, above and beyond its individual risk factors, for abnormal LV geometry. This aspect was examined in 830 asymptomatic patients (mean age 37 years, 69% whites, 41% men) as a part of the Bogalusa Heart Study. Patients with MS (as defined by the National Cholesterol Education Program Adult Treatment Panel III) showed adverse levels of echocardiographic parameters compared with patients without MS. With respect to MS components, patients with eccentric hypertrophy (EH) or concentric hypertrophy (CH) showed higher values of MS risk factors compared with patients with normal geometric pattern but no differences were noted between patients with normal and concentric remodeling. Of note, patients with concentric remodeling versus EH showed significantly higher systolic and diastolic blood pressure and fasting glucose levels. A model including only MS strongly predicted risk of EH (odds ratio [OR] 4.16, p <0.0001) and CH (OR 13.6, p <0.0001) compared with normal LV geometry. In a model including only individual MS risk factors, obesity (EH vs normal OR 14.4, p <0.0001), high blood pressure (CH vs normal OR 19.38, p <0.0001), and high fasting glucose levels (CH vs normal OR 4.02, p = 0.001) were significant predictors of abnormal LV geometry. However, the likelihood ratio test and comparisons of C-statistics for models including only individual MS risk factors versus models also including the MS variable were not significant. In conclusion, MS and its individual risk factors were strongly associated with LV geometry. However, MS did not predict risk of abnormal LV geometry independent of its individual components.

Read more
Authors
; ; ;
Related with
Citations(7)
Diseases(1)
Organisms(1)
Anatomy(1)
Clinical trials(1)
Authors(4)
Pubmed
Progression of segment-specific carotid artery intima-media thickness in young adults (from the Bogalusa Heart Study).
Journal: The American journal of cardiology
February/7/2011
Description

Carotid intima-media thickness (CIMT) progression is predictive of future cardiovascular events in middle-age and older adults. However, information is scant on segment-specific CIMT progression by race (black vs white) and gender and its predictors during short-term follow-up in asymptomatic young adults. B-mode ultrasound images of the far walls of both carotid arteries were obtained in 842 subjects aged 24 to 43 years and enrolled in the Bogalusa Heart Study (70% whites and 42% men). The CIMT and cardiometabolic risk variables were measured at baseline and after an average of 2.4 years. The mean CIMT progression rates/year adjusted for age, race, and gender were greatest at the bulb, followed by the internal and common carotid segments (p <0.0001). In a multivariate logistic model, age, mean arterial pressure, and high-density lipoprotein cholesterol were significantly associated with common CIMT progression. Smoking, age, insulin resistance index, and mean arterial pressure were significantly associated with bulb CIMT progression; and the waist/height ratio, smoking, age, and mean arterial pressure were significantly associated with internal CIMT progression, independent of the baseline CIMT and traditional cardiometabolic risk variables, including adiponectin, C-reactive protein, and intercellular adhesion molecules. In addition, the status of progression was associated with a greater prevalence of metabolic syndrome (common and internal CIMT, p <0.05; bulb CIMT, p <0.0001) and diabetes (bulb CIMT only, p <0.001). In conclusion, in younger adults, the magnitude of progression of CIMT within a short period varied in a segment-specific manner, regardless of race or gender, and was predictable using modifiable traditional risk factors. This could have implications for preventive and interventional cardiology.

Read more
Authors
; ; ; ; ;
Related with
Citations(7)
Organisms(1)
Anatomy(3)
Affiliates(2)
Clinical trials(1)
Authors(6)
Pubmed
Protective properties of tanshinone I against oxidative DNA damage and cytotoxicity.
Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
August/14/2014
Description

Tanshinone I, a naturally occurring diterpene from Danshen, has been shown to possess hepatocyte protective, anticancer, and memory enhancing properties. However, there are few stringent pharmacological tests for neuroprotection of tanshinone I thus far. Since peroxynitrite is involved in the pathogenesis of neurodegenerative disorders, this study was undertaken to investigate whether the neuroprotective effect of tanshinone I is associated with inhibition of peroxynitrite-caused DNA damage, a critical event leading to peroxynitrite-induced cytotoxicity. Our results show that tanshinone I can significantly inhibit peroxynitrite-induced DNA damage both in φX-174 plasmid DNA and rat primary astrocytes. EPR spectroscopy indicates that tanshinone I potently diminished the DMPO-hydroxyl radical adduct signal from peroxynitrite. Taken together, these results demonstrate for the first time that tanshinone I can protect against peroxynitrite-induced DNA damage, hydroxyl radical formation and cytotoxicity, which might have implications for tanshinone I-mediated neuroprotection.

Read more
Authors
; ; ;
Related with
Citations(3)
Drugs or chemicals(4)
Organisms(3)
Processes(1)
Anatomy(2)
Affiliates(1)
Authors(4)
Pubmed
Comparison and analysis of the genomes of two Aspergillus oryzae strains.
Journal: Journal of agricultural and food chemistry
July/6/2014
Description

A. oryzae 3.042 (China) and A. oryzae RIB40 (Japan) used for soy sauce fermentation show some regional differences. We sequenced the genome of A. oryzae 3.042 and compared it to A. oryzae RIB40 in an attempt to understand why different features are shown by these two A. oryzae strains. We predict 11,399 protein-coding genes in A. oryzae 3.042. The genomes of these two A. oryzae strains are collinear revealed by MUMmer analysis, indicating that the differences are not obvious between them. Several strain-specific genes of two strains are identified by genome sequences' comparison, and they are classified into some groups, which have the relationship with cell growth, cellular response and regulation, resistance, energy metabolism, salt tolerance, and flavor formation. A. oryzae 3.042 showed stronger potential for mycelial growth and environmental stress resistance, such as the genes of chitinase and quinone reductase. Some genes unique to A. oryzae RIB40 were related to energy metabolism and salt tolerance, especially genes for Na(+) and K(+) transport, while others were associated with signal transduction and flavor formation. The genome sequence of A. oryzae 3.042 will facilitate the identification of the genetic basis of traits in A. oryzae 3.042, and accelerate our understanding of the different genetic traits of the two A. oryzae strains.

Read more
Authors
; ; ;
Related with
Citations(3)
Drugs or chemicals(1)
Organisms(1)
Processes(2)
Affiliates(1)
Authors(4)
Pubmed
G-6A polymorphism of the angiotensinogen gene and its association with left ventricular mass in asymptomatic young adults from a biethnic community: the Bogalusa Heart Study.
Journal: American journal of hypertension
December/20/2005
Description

BACKGROUND

Angiotensinogen (AGT), the precursor of angiotensin II and a rate limiting factor in the renin-angiotensin system, is implicated in left ventricular hypertrophy, as angiotensin II is a potent stimulator of cardiac growth. A genetic variant (G-6A) in the proximal promoter region of the AGT gene may be particularly important for changes in left ventricular mass (LVM). However, previous findings associating this variant with LVM among relatively older adults with cardiovascular morbidity are inconsistent and contradictory.

METHODS

This study examined the effect of G-6A polymorphism on LVM in a biethnic (African American and white), community-based sample of 362 asymptomatic younger adults (mean age 32.5 years, 71% white, 39% male). Two-dimensional M-mode echocardiography was used to assess LVM, and the indexation of LVM for height(2.7) (LVMI) was used to adjust for body size.

RESULTS

The frequency of the variant A(-6) allele was higher in subjects of African American than in white ethnicity (0.819 v 0.439, P < .0001), with carriers (GA+AA) representing 97.0% of African American as compared to 66.6% of white subjects. After adjusting for age, ethnicity (for total sample), gender, body mass index, systolic BP, and homeostasis model assessment index of insulin resistance, mean levels for both LVM and LVMI showed a significant decreasing trend with increasing gene dosage of the A(-6) allele in white subjects, African American subjects, and the total sample.

CONCLUSIONS

These results indicate that the A(-6) allele frequency differs markedly between African Americans and white individuals, and that the variant allele modulates LVM and LVMI in a beneficial manner in asymptomatic young adults of white and African American ethnicities.

Read more
Authors
; ; ; ; ; ;
Related with
Citations(3)
Genes(1)
Drugs or chemicals(1)
Diseases(2)
Organisms(1)
Processes(4)
Anatomy(1)
Affiliates(1)
Clinical trials(1)
Authors(7)
Pubmed
Computed tomographic morphometric analysis of the pediatric occipital condyle for occipital condyle screw placement.
Journal: Spine
October/22/2014
Description

METHODS

This study is a computed tomographic (CT)-based morphometric analysis of the pediatric occipital condyles as related to occipital condyle screw placement.

OBJECTIVE

To quantify reference data concerning the dimensions of the immature occipital condyles to guide the placement of occipital condyle screw.

BACKGROUND

To the best of our knowledge, no published study has provided insight into the anatomy of occipital condyle of the pediatric population with different age groups.

METHODS

Sixty-nine pediatric patients were divided into 4 age groups, and their occipital condyles were studied on CT scans. Condylar length, width, height, sagittal angle, and sagittal angle lengths were measured on Philips Brilliance 16 CT.

RESULTS

The mean pediatric coronal height, sagittal length, and axial width noted statistically significant age-related differences were 9.0 mm, 21.3 mm, and 9.8 mm, respectively. The mean sagittal angle for all patients was 27.2 ± 5.1° (range, 15.1-41.0°). In 82.6% (114/138) of the occipital condyles, the anatomy could accept the occipital condyle screw (width ≥8 mm and height ≥6.5 mm).

CONCLUSIONS

Our investigation provides insight into the anatomy of occipital condyle of the pediatric population with different age groups. As the pediatric occipital condyles have sufficient occipital bone for appropriate fixation or fusion, the occipital condyle screws fixation is a feasible technique for children. Even so, given the evolution of this technique being still in its infancy and the complexity inherent to the craniovertebral junction, a careful radiological analysis of occipital condyle must be required in preoperative planning and feasibility determination.

METHODS

N/A.

Read more
Authors
; ; ; ; ; +2 authors
Related with
Citations(4)
Organisms(1)
Anatomy(2)
Authors(8)
Pubmed
Glyco-nanoparticles with sheddable saccharide shells: a unique and potent platform for hepatoma-targeting delivery of anticancer drugs.
Journal: Biomacromolecules
October/30/2014
Description

Reduction-sensitive shell-sheddable glyco-nanoparticles were designed and developed based on poly(ε-caprolactone)-graft-SS-lactobionic acid (PCL-g-SS-LBA) copolymer for efficient hepatoma-targeting delivery of doxorubicin (DOX). PCL-g-SS-LBA was prepared by ring-opening copolymerization of ε-caprolactone and pyridyl disulfide carbonate followed by postpolymerization modification with thiolated lactobionic acid (LBA-SH) via thiol-disulfide exchange reaction. The dynamic light scattering (DLS) and transmission electron microscopy (TEM) showed that PCL-g-SS-LBA was self-assembled into monodisperse nanoparticles (SS-GNs) with a mean diameter of about 80 nm. SS-GNs while remaining stable under physiological conditions (37 °C, pH 7.4) were prone to rapid shell-shedding and aggregation in the presence of 10 mM dithiothreitol (DTT). DOX was loaded into SS-GNs with a decent loading content of 12.0 wt %. Notably, in vitro release studies revealed that about 80.3% DOX was released from DOX-loaded SS-GNs in 24 h under a reductive condition while low drug release (<21%) was observed for DOX-loaded PCL-g-LBA nanoparticles (reduction-insensitive control) under otherwise the same condition and for DOX-loaded SS-GNs under a nonreductive condition. The flow cytometry and confocal microscopy observations indicated that SS-GNs were efficiently taken up by asialoglycoprotein receptor (ASGP-R)-overexpressing HepG2 cells likely via a receptor-mediated endocytosis mechanism and DOX was released into the nuclei of cells following 4 h incubation. MTT assays showed that DOX-loaded SS-GNs exhibited a high antitumor activity toward HepG2 cells, which was comparable to free DOX and about 18-fold higher than their reduction-insensitive counterparts, while blank SS-GNs were nontoxic up to a tested concentration of 1.0 mg/mL. These shell-sheddable glyco-nanoparticles are promising for hepatoma-targeting chemotherapy.

Read more
Authors
; ; ; ; ; ;
Related with
Citations(5)
Drugs or chemicals(5)
Diseases(2)
Organisms(1)
Processes(1)
Anatomy(1)
Affiliates(1)
Authors(7)
Pubmed
Variants in the 5'-upstream region of GPC5 confer risk of lung cancer in never smokers.
Journal: Cancer epidemiology
November/1/2014
Description

BACKGROUND

A recent genome-wide study (GWAS) has identified GPC5 as a promising susceptibility gene for Lung cancer in never smokers (LCINS). However, the most significant single nucleotide polymorphism (SNP) in this GWAS, rs2352028, has yielded controversial results. The aim of this study was to clarify the relationship between rs2352028 and LCINS. Considering that rs2352028 might be largely marker-SNP correlated to causative variants, two predicted functional SNPs, rs3759452 and rs7322083, were additionally investigated in this study.

METHODS

A hospital based case-control study including 298 cases and 599 controls in a never-smoking Chinese Han population was conducted, and then a meta-analysis combining our data and published data was performed to verify the findings.

RESULTS

The SNP rs3759452, predicted to potentially change transcription factor binding site of GPC5, was significantly associated with LCINS risk (odds ratio for dominant model=1.55, 95% confidence interval=1.14-2.12). Nevertheless, no significant evidence was showed for rs2352028, both in our case-control study and the meta-analysis including 13 studies of 2342 LCINS cases and 13,398 never-smoking controls. Further subgroup meta-analysis according to population ethnicity and cancer histology also reported no significant association of rs2352028.

CONCLUSIONS

The association conferring rs3759452 further supports the value of GPC5 in susceptibility to LCINS. Nevertheless, comprehensive analyses are warranted to dissect the functional mechanism underpinning rs3759452.

Read more
Authors
; ; ; ; ; +6 authors
Related with
Citations(5)
Genes(1)
Drugs or chemicals(1)
Diseases(2)
Organisms(1)
Processes(1)
Affiliates(4)
Authors(12)
Pubmed
Regulatory properties of malic enzyme in the oleaginous yeast, Yarrowia lipolytica, and its non-involvement in lipid accumulation.
Journal: Biotechnology letters
May/27/2014
Description

Malic enzyme (EC 1.1.1.40) converts L-malate to pyruvate and CO2 providing NADPH for metabolism especially for lipid biosynthesis in oleaginous microorganisms. However, its role in the oleaginous yeast, Yarrowia lipolytica, is unclear. We have cloned the malic enzyme gene (YALI0E18634g) from Y. lipolytica into pET28a, expressed it in Escherichia coli and purified the recombinant protein (YlME). YlME used NAD(+) as the primary cofactor. Km values for NAD(+) and NADP(+) were 0.63 and 3.9 mM, respectively. Citrate, isocitrate and α-ketoglutaric acid (>5 mM) were inhibitory while succinate (5-15 mM) increased NADP(+)- but not NAD(+)-dependent activity. To determine if fatty acid biosynthesis could be increased in Y. lipolytica by providing additional NADPH from an NADP(+)-dependent malic enzyme, the malic enzyme gene (mce2) from an oleaginous fungus, Mortierella alpina, was expressed in Y. lipolytica. No significant changes occurred in lipid content or fatty acid profiles suggesting that malic enzyme is not the main source of NADPH for lipid accumulation in Y. lipolytica.

Read more
Authors
; ; ; ; ; ;
Related with
Citations(8)
Drugs or chemicals(4)
Organisms(2)
Processes(2)
Affiliates(1)
Authors(7)
load more...