Wei Chen
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Publication
Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
June/11/2018
Abstract
An in vitro synthesized random ssDNA library was subjected to 12 rounds of selection against anti-screening cells and sieving cells by SELEX. Normal and inflammatory cervical exfoliation cells were selected as anti-screening cells, and the cervical exfoliation cells of low-grade squamous intraepithelial lesion (CIN1), high-grade squamous intraepithelial lesion (CIN2, CIN3) and cervical carcinoma were selected as sieving cells during the screening process. Then, the highly specific aptamer CIN-Ap4 was established by the analysis of the specificity, affinity and cell immunofluorescence, which can be used as biomarker for Cervical Intraepithelial Neoplasia. Prime Premier 5.0 was applied to design a random ssDNA library. According to the fixed sequence at both ends of the library, a pair of primers were designed and synthesized. At the same time, the optimal annealing temperature, cycle times and primer concentration ratio of PCR procedure were selected. The results under the optimal condition are shown as follows. In the 50 μL reaction system, the optimum reaction conditions of symmetry PCR are as follows: annealing temperature is 49.5 ℃, number of cycles is 15. The optimal reaction conditions of indirect asymmetric PCR are as follows: the primer concentration ratio is 80:1, and the number of cycles is 35. The experiment proves that the oligonucleotide library is constructed successfully, and the highly specific dsDNA and ssDNA can be obtained under optimal PCR conditions with good repeatability, which establishes the foundation for the further exploration and experimentation.
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Journal: Angewandte Chemie (International ed. in English)
December/6/2018
Abstract
The approach of concurrent-to-synchronous chemoradiation has now been advanced by well-designed nanovesicles that permit X-ray irradiation-triggered instant drug release. The nanovesicles consist of Au nanoparticles tethered with irradiation labile linoleic acid hydroperoxide (LAHP) molecules and oxidation-responsive poly(propylene sulfide)-poly(ethylene glycol) (PPS-PEG) polymers, where DOX were loaded in the inner core of the vesicles (Au-LAHP-vDOX). Upon irradiation, the in situ formation of hydroxyl radicals from LAHP molecules triggers the internal oxidation of PPS from being hydrophobic to hydrophilic, leading to degradation of the vesicles and burst release of cargo drugs. In this manner, synchronous chemoradiation showed impressive anticancer efficacy both in vitro and in a subcutaneous mouse tumor model by one-dose injection and one-time irradiation.
Publication
Journal: Genetic epidemiology
November/11/2018
Abstract
In a genome-wide association study (GWAS), association between genotype and phenotype at autosomal loci is generally tested by regression models. However, X-chromosome data are often excluded from published analyses of autosomes because of the difference between males and females in number of X chromosomes. Failure to analyze X-chromosome data at all is obviously less than ideal, and can lead to missed discoveries. Even when X-chromosome data are included, they are often analyzed with suboptimal statistics. Several mathematically sensible statistics for X-chromosome association have been proposed. The optimality of these statistics, however, is based on very specific simple genetic models. In addition, while previous simulation studies of these statistics have been informative, they have focused on single-marker tests and have not considered the types of error that occur even under the null hypothesis when the entire X chromosome is scanned. In this study, we comprehensively tested several X-chromosome association statistics using simulation studies that include the entire chromosome. We also considered a wide range of trait models for sex differences and phenotypic effects of X inactivation. We found that models that do not incorporate a sex effect can have large type I error in some cases. We also found that many of the best statistics perform well even when there are modest deviations, such as trait variance differences between the sexes or small sex differences in allele frequencies, from assumptions.
Publication
Journal: Animal reproduction science
November/2/2014
Abstract
This research was conducted to determine the effect of heat stress on the nutritional metabolism and productivity of egg-laying shelducks. Healthy shelducks (n=120) in the early laying stage (uniform body weights and normal feed intakes) were randomly assigned to two identical climate chambers and exposed to constant high temperature (34°C) or control temperature (23°C) for 28d. The heat-exposed ducks had reduced feed intakes and laying rates (P<0.05), increased frequency of panting and spreading wings and dull featheration; egg weight, eggshell thickness and strength, and Haugh unit also decreased and malondialdehyde (MDA) content of egg yolk increased (P<0.05). Compared with the control ducks, the plasma concentrations of HCO3(-), phosphorus, glucose, thyroxine and activities of glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase were decreased, while there were increased concentrations of corticosterone (P<0.05). The content of MDA and lactate in plasma and liver was greater in heat-exposed than in control ducks, but superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant enzymes (T-AOC) activities and glutathione (GSH) contents were less. The expression of HSP70 gene expression in the liver was increased in heat-stressed ducks. The relative weight of oviduct, number of large ovarian follicles, length of the oviduct all decreased (P<0.05) in heat-treated ducks, as did expression of carbonic anhydrase and calcium binding protein genes in the shell gland as a result of heat stress. In summary, heat stress decreased the productivity of ducks, which related to reduced feed intake, protein synthesis, endocrine dysfunction, less antioxidant capacity, and derangement of calcium and phosphorous balance.
Publication
Journal: Inorganic chemistry
March/19/2017
Abstract
Humidity-induced single-crystal transformation was observed in the indium metal-organic polyhedra [In2(TCPB)2]·2H2O (In1), where H3TCPB is 1,3,5-tri(4-carboxyphenoxy)benzene. When the humidity is above 58% relative humidity (RH) at room temperature, the neutral compound In1 could be successfully converted into the positively charged compound In1-H along with the color change from yellow to deep red, which also undergoes a reversible transformation into In1 driven by thermal dehydration. Notably, the color of In1 takes only 5 min to change under 58% RH at room temperature, which is much quicker than common desiccant bluestone. As the water content is increased from 0.0% to 0.2% in acetonitrile solvent, compound In1 exhibits rapid detection of trace amounts of water through turn-off luminescence sensing mechanism with a low detection limit of 2.95 × 10-4%. Because of the formation of extensive hydrogen-bonding network between the metal-organic polyhedra (MOPs) and surrounding guest OH- ions, compound In1-H, along with isostructural Ga1-H, displays excellent proton conductivity up to 2.84 × 10-4 and 2.26 × 10-4 S cm-1 at 298 K and 98% RH, respectively. Furthermore, the activation energies are found to be 0.28 eV for In1-H and 0.34 eV for Ga1-H. This method of incorporation of OH- ions to obtain high proton conductivity MOPs with low activation energy demonstrates the advantage of OH- ion conduction in the solid-state materials.
Publication
Journal: Molecular therapy. Nucleic acids
November/13/2018
Abstract
RNA modifications are additions of chemical groups to nucleotides or their local structural changes. Knowledge about the occurrence sites of these modifications is essential for in-depth understanding of the biological functions and mechanisms and for treating some genomic diseases as well. With the avalanche of RNA sequences generated in the post-genomic age, many computational methods have been proposed for identifying various types of RNA modifications one by one. However, so far no method whatsoever has been developed for simultaneously identifying several different types of RNA modifications. To address such a challenge, we developed a predictor called "iRNA-3typeA," by which we can simultaneously identify the occurrence sites of the following three most frequently observed modifications in RNA: (1) N1-methyladenosine (m1A), (2) N6-methyladenosine (m6A), and (3) adenosine to inosine (A-to-I). It has been shown via rigorous cross-validations for the RNA sequences from Homo sapiens and Mus musculus transcriptomes that the success rates achieved by the powerful new predictor are quite high. For the convenience of broad experimental scientists, a user-friendly web server for iRNA-3typeA has been established at http://lin-group.cn/server/iRNA-3typeA/. It is anticipated that iRNA-3typeA may become a useful high throughput tool for genome analysis.
Publication
Journal: Physical chemistry chemical physics : PCCP
May/22/2018
Abstract
Using density functional theory (DFT) calculations, we have comprehensively investigated the structure and the hydrogen evolution reaction (HER) catalytic activity for pristine and monovacancy defected WP systems. It was revealed that the (101) surface can have the most exposure for the WP structure. The calculated free energy values of H* (ΔGH*) show that the (101) surface can exhibit good HER catalytic activity, where the top site over the W atoms can act as the most active site for HER due the existence of antibonding characteristics after adsorbing H*. Moreover, we have proposed an effective strategy through the introduction of a monovacancy to further improve the HER activity of the WP system. It was found that the formation of the W-monovacancy can significantly improve HER activity since the decreased coordination number of the correlative atoms brings some new active sites around the defect. Particularly, these systems can even exhibit considerably high HER activity over a wide range of hydrogen coverage. Clearly, all these fascinating findings at the atomic level can be beneficial for realizing highly efficient nonprecious HER electrocatalysts based on tungsten phosphide and even other transition metal phosphides in the near future.
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Journal: Environmental science & technology
November/25/2018
Abstract
The status of defects of TiO2 are of fundamental importance in determining its physicochemical properties. Here we report a simple chemical deposition method for controllable synthesis of defective anatase TiO2 nanocrystals under various calcination atmospheres. XPS and ESR analysis reveals that both the oxygen vacancies ( VO) and the trivalent titanium (Ti3+) defects exist in TiO2 after N2 treatment (N-TiO2). Meanwhile, mainly VO defects can be obtained in TiO2 with air calcination (A-TiO2). ESR spectra for reactive oxygen species determination, clearly show that the visible light catalytic activity is mainly caused by the efficient activation of oxygen molecules to •O2- species for A-TiO2, which play an important role in hindering the accumulation of intermediates during p-chlorophenol (4-CP) photodegradation process. However, the oxygen molecules cannot be activated for N-TiO2 even with superior visible light absorption and thus the photogenerated electron are reductant, which participated in the transformation of BQ to HQ via electron shuttle mechanism. Moreover, A-TiO2 exhibits higher separation efficiency of photogenerated carriers than that of N-TiO2, showing the critical role of VO with a suitable concentration in transferring photogenerated charges.
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Journal: American journal of physiology. Gastrointestinal and liver physiology
March/28/2019
Abstract
Elimination or suppression of causative factors can raise the possibility of liver fibrosis regression. However, different injurious stimuli will give fibrosis from somewhat different etiologies, which, in turn, may hamper the discovery of liver fibrosis-specific therapeutic drugs. Therefore, the analogical cellular and molecular events shared by various etiology-evoked liver fibrosis should be clarified. Our present study systematically integrated five publicly available transcriptomic data sets regarding liver fibrosis with different etiologies from the Gene Expression Omnibus database and performed a series of bioinformatics analyses and experimental verifications. A total of 111 significantly upregulated and 16 downregulated genes were identified specific to liver fibrosis independent of any etiology. These genes were predominately enriched in some Kyoto Encyclopedia of Genes and Genomes pathways, including the "PI3K-AKT signaling pathway," "Focal adhesion," and "ECM-receptor interaction." Subsequently, five prioritized liver fibrosis-specific genes, including COL4A2, THBS2, ITGAV, LAMB1, and PDGFRA, were screened. These genes were positively associated with each other and liver fibrosis progression. In addition, they could robustly separate all stages of samples in both training and validation data sets with diverse etiologies when they were regarded as observed variables applied to principal component analysis plots. Expressions of all five genes were confirmed in activated primary mouse hepatic stellate cells (HSCs) and transforming growth factor β1-treated LX-2 cells. Moreover, THBS2 protein was enhanced in liver fibrosis rodent models, which could promote HSC activation and proliferation and facilitate NOTCH1/JAG1 expression in HSCs. Overall, our current study may provide potential targets for liver fibrosis therapy and aid to a deeper understanding of the molecular underpinnings of liver fibrosis. NEW & NOTEWORTHY Prioritized liver fibrosis-specific genes THBS2, COL4A2, ITGAV, LAMB1, and PDGFRA were identified and significantly associated with liver fibrosis progression and could be combined to discriminate liver fibrosis stages regardless of any etiology. Among the identified prioritized liver fibrosis-specific targets, THBS2 protein was confirmed to be enhanced in liver fibrosis rodent models, which could promote hepatic stellate cell (HSC) activation and proliferation and facilitate NOTCH1/JAG1 expression in HSCs.
Publication
Journal: IEEE transactions on visualization and computer graphics
November/25/2018
Abstract
We propose an approach to analyzing data in which texts are associated with spatial and temporal references with the aim to understand how the text semantics vary over space and time. To represent the semantics, we apply probabilistic topic modeling. After extracting a set of topics and representing the texts by vectors of topic weights, we aggregate the data into a data cube with the dimensions corresponding to the set of topics, the set of spatial locations (e.g., regions), and the time divided into suitable intervals according to the scale of the planned analysis. Each cube cell corresponds to a combination (topic, location, time interval) and contains aggregate measures characterizing the subset of the texts concerning this topic and having the spatial and temporal references within these location and interval. Based on this structure, we systematically describe the space of analysis tasks on exploring the interrelationships among the three heterogeneous information facets, semantics, space, and time. We introduce the operations of projecting and slicing the cube, which are used to decompose complex tasks into simpler subtasks. We then present a design of a visual analytics system intended to support these subtasks. To reduce the complexity of the user interface, we apply the principles of structural, visual, and operational uniformity while respecting the specific properties of each facet. The aggregated data are represented in three parallel views corresponding to the three facets and providing different complementary perspectives on the data. The views have similar look-and-feel to the extent allowed by the facet specifics. Uniform interactive operations applicable to any view support establishing links between the facets. The uniformity principle is also applied in supporting the projecting and slicing operations on the data cube. We evaluate the feasibility and utility of the approach by applying it in two analysis scenarios using geolocated social media data for studying people's reactions to social and natural events of different spatial and temporal scales.
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Journal: Journal of diabetes investigation
November/13/2018
Abstract
UNASSIGNED
Previous studies have shown that glucose peak time during the oral glucose tolerance test varies in type 2 diabetes patients; however, characteristics of this heterogeneity remain unclear. This research aimed to investigate the characteristics of delayed glucose peak time in type 2 diabetes.
METHODS
A total of 178 participants who underwent the oral glucose tolerance test were divided into five groups according to glucose peak time.
RESULTS
A total of 25 participants with normal glucose tolerance had a glucose peak at 30 min. Among participants with type 2 diabetes, 28 had a glucose peak at 60 min, 48 at 90 min, 45 at 120 min and 32 at 150 min. With the glucose peak time delayed, glycated hemoglobin, area under the glucose curve and homeostatic model assessment of insulin resistance increased gradually (P = 0.038, P < 0.0001, P < 0.0001, respectively), and oral glucose insulin sensitivity, homeostatic model assessment of β-cell function, insulinogenic index, modified β-cell function index and disposition indices decreased (P < 0.0001 for all). On multinominal logistic regression, insulinogenic index (odds ratio 0.73, 95% confidence interval 0.57-0.93, P = 0.01), modified β-cell function index (odds ratio 0.67, 95% confidence interval 0.47-0.94, P = 0.023) and oral glucose insulin sensitivity (odds ratio 0.91, 95% confidence interval 0.87-0.96, P < 0.0001) were independently correlated with delayed glucose peak time.
CONCLUSIONS
Delay in glucose peak time indicated an increase in blood glucose and a decrease in insulin sensitivity and secretion. Furthermore, insulinogenic index, modified β-cell function index and oral glucose insulin sensitivity contributed to delayed glucose peak time.
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Journal: Frontiers in cellular neuroscience
November/13/2018
Abstract
Variants near the ATP-binding cassette transporter A1 (ABCA1) gene are associated with elevated intraocular pressure and newly discovered risk factors for glaucoma. Previous studies have shown an association between ABCA1 deficiency and retinal inflammation. Using a mouse model of ischemia-reperfusion (IR) induced by acute intraocular pressure elevation, we found that the retinal expression of ABCA1 protein was decreased. An induction of ABCA1 expression by liver X receptor agonist TO901317 reduced retinal ganglion cell (RGC) apoptosis after IR and promoted membrane translocation and secretion of the anti-inflammatory factor annexin A1 (ANXA1). Moreover, ABCA1 and ANXA1 co-localized in cell membranes, and the interaction domain is amino acid 196 to 274 of ANXA1 fragment. TO901317 also reduced microglia migration and activation and decreased the expression of pro-inflammatory cytokines interleukin (IL)-17A and IL-1β, which could be reversed by the ANXA1 receptor blocker Boc2. Overexpression of TANK-binding kinase 1 (TBK1) increased ABCA1 degradation, which was reversed by the proteasome inhibitor carbobenzoxy-L-leucyl-L-leucyl-L-leucinal (MG132). Silencing Tbk1 with siRNA increased ABCA1 expression and promoted ANXA1 membrane translocation. These results indicate a novel IR mechanism, that leads via TBK1 activation to ABCA1 ubiquitination. This degradation decreases ANXA1 secretion, thus facilitating retinal inflammation and RGC apoptosis. Our findings suggest a potential treatment strategy to prevent RGC apoptosis in retinal ischemia and glaucoma.
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Journal: Journal of cellular physiology
November/13/2018
Abstract
Recent comprehensive next-generation genome and transcriptome analyses in lung cancer patients, several clinical observations, and compelling evidence from mouse models of lung cancer have uncovered a critical role for Notch signaling in the initiation and progression of non-small-cell lung cancer (NSCLC). Notably, Rumi is a "protein O-glucosyltransferase" that regulates Notch signaling through O-glucosylation of Notch receptors, and is the only enzymatic regulator whose activity is required for both ligand-dependent and ligand-independent activation of Notch. We have conducted a detailed study on RUMI's involvement in NSCLC development and progression, and have further explored the therapeutic potential of its targeting in NSCLC. We have determined that Rumi is highly expressed in the alveolar and bronchiolar epithelia, including club cells and alveolar type II cells. Remarkably, RUMI maps to the region of chromosome 3q that corresponds to the major signature of neoplastic transformation in NSCLC, and is markedly amplified and overexpressed in NSCLC tumors. Notably, RUMI expression levels are predictive of poor prognosis and survival in NSCLC patients. Our data indicates that RUMI modulates Notch activity in NSCLC cells, and that its silencing dramatically decreases cell proliferation, migration, and survival. RUMI downregulation causes severe cell cycle S-phase arrest, increases genome instability, and induces late apoptotic-nonapoptotic cell death. Our studies demonstrate that RUMI is a novel negative prognostic factor with significant therapeutic potential in NSCLC, which embodies particular relevance especially when considering that, while current Notch inhibitory strategies target only ligand-dependent Notch activation, a large number of NSCLCs are driven by ligand-independent Notch activity.
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Journal: OncoTargets and therapy
November/13/2018
Abstract
UNASSIGNED
RNF6, an E3 ligase, has been reported to play an important role in the tumorigenesis in several tissues, but its role in gastric cancer is still unknown. In this study, we aimed to investigate the biological function and molecular mechanisms of RNF6 in gastric cancer.
UNASSIGNED
The expression levels of RNF6 were detected by quantitative real-time PCR (qRT-PCR) and immunoblotting in gastric cancer tissues and cell lines. Cell Counting Kit-8 assay was performed to evaluate cell proliferation. Cell apoptosis was analyzed by flow cytometer and immunoblotting. Luciferase assay, immunoblotting and qRT-PCR were performed to explore the activation of STAT3. Immunoprecipitation was performed to evaluate the ubiquitination of SHP-1.
UNASSIGNED
In this study, RNF6 was found to be upregulated in both primary tissues and cell lines of gastric cancer. Knockdown or overexpression of RNF6 inhibited or promoted cell growth of gastric cancer cells. Knockdown of RNF6 also induced the cleavage of PARP and promoted cell apoptosis in gastric cancer cells. In addition, knockdown of RNF6 also increased the cytotoxicity of doxorubicin against gastric cancer. Moreover, knockdown of RNF6 inhibited STAT3-derived luciferase activity and downregulated the phosphorylation of STAT3, but upregulated the protein level of SHP-1. Knockdown of RNF6 downregulated the expression of MCL1 and XIAP, which are target genes of STAT3. Further studies showed that RNF6 regulated the stability of SHP-1 by inducing its polyubiquitination.
UNASSIGNED
These results demonstrated that RNF6 was highly expressed in gastric cancer and regulated the growth of gastric cancer cells by affecting SHP-1/STAT3 signaling, which suggested that RNF6 could be a novel target for gastric cancer therapy.
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Journal: The American journal of the medical sciences
November/17/2013
Abstract
BACKGROUND
The objective of this study was to determine the sex-specific association between the metabolic syndrome (MetS) and arterial stiffness.
METHODS
In this cross-sectional study, 3981 women and 4319 men aged 20 to 79 years were analyzed. All participants underwent the measurement of waist circumference, blood pressure (BP), brachial-ankle pulse wave velocity (baPWV) and blood chemistry. baPWV levels were compared between men and women using the Mann-Whitney's U test. Subjects with or without MetS and its components or specific clusters of MetS components in the different sexes were compared by the Kruskal-Wallis test. Binary logistic regression analysis was performed to evaluate the relationship between baPWV and the MetS components.
RESULTS
Women had lower baPWV than men in young and middle-aged subjects (P < 0.001), but there was no difference in the elderly subjects. baPWV levels in women were higher than in men with MetS and its components except for high-density lipoprotein cholesterol (HDL-C; P < 0.01). The combination of elevated triglycerides, elevated BP and elevated fasting glucose (with obesity or low HDL-C) had a greater baPWV than the other clusters. All the metabolic variables were positively correlated with baPWV except for HDL-C negatively correlating in women (P < 0.001), whereas age, BP and fasting glucose were moderately associated with baPWV in men (P < 0.001). Among the MetS components, BP had the strongest association with baPWV.
CONCLUSIONS
The MetS and its components affect arterial stiffness more severely in women than in men. More importance to women with MetS should be given.
Publication
Journal: Australasian physical & engineering sciences in medicine
October/16/2019
Abstract
There has been few studies focusing on the disc pressure of the upper thoracic spine and it still lacks the quantitative pressure measurement of each spinal disc segment. The aim of this study was to study the pressure changes of intervertebral disc in porcine upper thoracic spine using pressure-sensitive film. Twelve porcine thoracic motion segments were harvested and successively loaded with vertical loads of 100 N, 150 N, and 200 N during 5° of anterior flexion, 5° of posterior extension and 5° of lateral bending. The resulting pressure values were measured. During anterior flexion, the anterior annulus of all segments at all loads showed higher mean pressure values than those during vertical compression, whereas the posterior annulus did not show higher mean values. During posterior extension, the anterior annulus of all segments showed lower mean pressure values than those during vertical compression, whereas the posterior annulus did not show lower mean pressure values. During lateral bending, the annulus of all segments showed higher mean pressure values than those during vertical compression. The posterior thoracic vertebra plays an important role in the motion of the upper thoracic vertebral segment and pressure distribution. During lateral bending, the concave side pressure of the annulus increases obviously, suggesting that asymmetrical force is a contributory factor for scoliosis progression.
Publication
Journal: Biomedical and environmental sciences : BES
November/26/2012
Abstract
OBJECTIVE
To understand the current status and trends of tuberculosis mortality rates in China.
METHODS
In 2010, 161 National Disease Surveillance Points representing all 31 mainland provinces, municipalities, and autonomous regions of China collected tuberculosis mortality surveillance data, including age, sex, region, and type of tuberculosis (all, pulmonary, and extra-pulmonary). The mortality rates of the three types of tuberculosis were compared between 2004 and 2010.
RESULTS
In 2010, the mortality rates due to all tuberculosis, pulmonary tuberculosis, and extra-pulmonary tuberculosis were 4.69 (95% CI 4.54-4.84), 4.38 (4.23-4.52), and 0.31 (0.27-0.35) per 100 000 population, respectively. Mortality rates due to all tuberculosis and pulmonary tuberculosis were higher in males, the elderly, and those living in western and rural areas. From 2004 to 2010, the mortality rates due to all tuberculosis and pulmonary tuberculosis decreased by 36.02% and 37.70%, respectively, with an average annual rate of decline of 7.20% and 7.61%, respectively.
CONCLUSIONS
Mortality rates due to tuberculosis have declined rapidly in China. The target of reducing the 1990 mortality rate by 50% by 2015 has already been achieved. However, the tuberculosis control program should pay more attention to high-risk groups, including the elderly and those living in underdeveloped areas.
Publication
Journal: Clinical laboratory
December/14/2018
Publication
Journal: Yi chuan = Hereditas
November/20/2012
Abstract
In order to understand the structure and function of CuZnSOD gene, reveal the effect of the anti-oxidant in swine, and find the molecule marker correlated with meat traits, the cDNA of CuZnSOD gene was cloned and sequenced from muscle of Laiwu black swine by RACE (rapid amplification of cDNA end) techniques. The structure and function of CuZnSOD were analyzed by bioinformatics, and the gene expression profile in different tissues was examined by real-time PCR. The results showed that the full sequence of CuZnSOD cDNA is 658 bp (GU944822), containing 76 bp sequence of 5' UTR and 120 bp sequence of 3' UTR, and coding region (CDS, 462 bp) encodes 153 amino acids. The isoelectric point (pI) of the protein is 6.03, and the molecular weight is 15.9 kDa. There were one O-glycosylation site at the third amino acid and one N-glycosylation site at the eighty-fourth amino acid. The percentage of alpha helix was 1.31%. The alignment similarities of the CDS sequence of swine CuZnSOD with those of cattle, human, rat, and mouse were 87.74%, 87.66%, 83.44%, and 83.23%, and the similarities of amino acid sequence were 90.26%, 94.12%, 92.21%, and 91.50%, respectively. CuZnSOD possesses the typical metal binding ligands (GFHVHQFGDNT). The phylogenic tree based on CuZnSOD protein sequence detected the closest relationship between swine and cattle. CuZnSOD mRNA is a broad-spectrum expression gene, which was detected in brain, heart, spleen, liver, kidney, lung, large intestine, small intestine, spinal cord, muscle, backfat, and stomach. In particular, high expression levels of CuZnSOD mRNA were detected in kidney, small intestine and lung, but low expressions were observed in heart and muscle tissues.
Publication
Journal: Experimental and therapeutic medicine
November/12/2018
Abstract
Allograft rejection is an important issue post cardiac transplantation. In order to investigate the effect of combined treatment with simvastatin and rapamycin on allograft rejection, a cardiac transplantation rat model was employed in the present study. The survival time of rats following cardiac transplantation was recorded, while histopathological alterations were assessed by hematoxylin and eosin staining. The levels of transcription factors were measured by reverse transcription-quantitative polymerase chain reaction. In addition, the levels of CD4+ interleukin (IL)-17+ cells and CD4+ forkhead box P3 (FOXP3)+ cells in the allografts and CD4+ T cells and CD8+ T cells in the spleens were detected by flow cytometry. The results of the current study demonstrated that, following treatment with simvastatin and rapamycin, the survival time of model rats was prolonged, and the histopathological damage was attenuated. Treatment with simvastatin and rapamycin also led to decreased retinoic acid receptor-related orphan receptor γt (RORγt) level, increased FOXP3 level, reduced levels of CD4+IL-17+, CD4+ T and CD8+ T cells, and increased level of CD4+FOXP3+ cells. In conclusion, the current study observed that simvastatin and rapamycin performed a synergistic effect to reduce cardiac transplantation rejection. Thus, combined therapy of simvastatin and rapamycin may be a promising adjuvant therapy to reduce rejection post cardiac transplantation.
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Journal: Digestive surgery
May/17/2012
Abstract
OBJECTIVE
With the development of early diagnostic technologies, more synchronous colorectal cancers (SCRCs) can be clinically detected. Although SCRCs are recognized as a significant clinical entity, their clinical features, diagnosis, treatment, and prognosis have yet to be definitively established. In order to obtain a comprehensive understanding of this disease and to establish an efficient profile by which to recognize individuals at high risk of developing SCRCs, we carried out a review of the relevant literature.
METHODS
The PubMed database was searched for publications of 'synchronous colorectal carcinoma/cancer/adenocarcinoma' and 'multiple colorectal carcinomas'. All publications up to January 2011 were considered, and then only articles in English were retrieved for inclusion in this review.
RESULTS
The incidence of SCRCs was found to be higher in older and male patients. The prognosis in patients with SCRCs was equivalent to that in patients with solitary CRC. The failure to diagnose synchronous lesions before and during the operation was associated with repeated surgery.
CONCLUSIONS
SCRCs possess distinctive features compared to solitary CRC. While all colorectal patients should be carefully assessed to rule out the presence of concurrent colon adenomas, since missed lesions can result in additional surgery and poor prognosis, particular attention should be given to the high-risk group of older male patients.
Publication
Journal: CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne
April/6/2009
Publication
Journal: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery
January/6/2010
Abstract
OBJECTIVE
To investigate the influence of biological behavior of epithelial cells on the hair follicles and sebaceous glands (HFSG) structure in keloids (K).
METHODS
The expression of intercellular adhesion molecule (ICAM)-1, D-related human leucocyte antigen (HLA-DR), and cytokeratin (CK) 14 on epithelial cells and the amount of activity T-lymphocytes were detected in specimens of keloid edge and normal skin with immunohistochemical and histological methods.
RESULTS
In comparison with normal skin specimens, epithelial cells were proliferated in K-HFSG presented structural aberration and disintegrate or abnormally to form solid-epithelial island-like structure, and the density of HSFG with hyperplasia and the ageing scar in keloids was apparently decreased. They strongly expressed ICAM-1, HLA-DR, and CK14 in the epithelial cells, there were many immunologic cells which expressed CD4, CD45RO, and interferon (IFN)-gamma around the K-HFSG. The expressed level of epithelial cells was positively correlated with the density of immunologic cells nearby K-HFSG.
CONCLUSIONS
It could be concluded that the reactivity with hyperplasia and immunoinduction of epithelial cells might be associated with the destruction of the some HFSG structure in the keloids.
Publication
Journal: Chemical communications (Cambridge, England)
November/13/2019
Abstract
A target triggered dual-DNA machine, consisting of one rolling circle amplification (RCA)-based circular DNA machine (RCA-CDM) and one cyclical strand displacement amplification (CSDA)-based bidirectional DNA machine (CSDA-BDM), was developed for robust miRNA determination.
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