Abstract:

Objective: Diclofenac is a non-steroidal anti-inflammatory drug linked with considerable organ toxicity caused via increased generation of reactive oxygen species. We evaluated whether the antioxidant effect of virgin coconut oil (VCO) could prevent diclofenac-induced oxidative nephrotoxicity in rats.

Materials and methods: Randomized rats were pre-supplemented orally with VCO (5 or 10 ml/kg body weight) from day 1 to 24, and injected with normal saline or diclofenac (100 mg/kg) from day 22 to day 24 intraperitoneally.

Results: Diclofenac significantly (p<0.05) increased serum urea and creatinine levels. Renal tumor necrosis factor-α (TNF-α) and malondialdehyde (MDA) levels markedly (p<0.05) increased, whereas renal glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) activities considerably (p<0.05) decreased compared to normal control. Histopathological alterations were caused by diclofenac. However, treatment with oral VCO for 21 days prior to diclofenac administration, attenuated histological renal damage, and restored antioxidant enzyme activities and TNF-α levels in kidney.

Conclusion: These findings revealed that VCO has potential benefits to prevent diclofenac-induced nephrotoxic damage.

Keywords: Antioxidants; Diclofenac; Nephrotoxicity; Oxidative stress; Virgin coconut oil.

Open in

PUBMED | Google Scholar | Wikipedia

Relations:

Content

Citations

(1)

References

(26)

Conditions

(2)

Drugs

(2)

Chemicals

(8)

Processes

(1)

Nephroprotective activity of virgin coconut oil on diclofenac-induced oxidative nephrotoxicity is associated with antioxidant and anti-inflammatory effects in rats

Chima Ekeleme-Egedigwe

Janet Oluniran

Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria

Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria

Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria

Department of Biochemistry, Faculty of Science, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria

Ministry of Agriculture and Rural Development, Ebonyi State Agricultural Development Programme, Abakaliki, Nigeria

^{Corresponding Author: Tel: +2348030717151, ademola.famurewa@funai.edu.ng}

Received 2019 Jul 2; Revised 2019 Aug 10; Accepted 2019 Aug 31.

Copyright notice

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective:

Diclofenac is a non-steroidal anti-inflammatory drug linked with considerable organ toxicity caused via increased generation of reactive oxygen species. We evaluated whether the antioxidant effect of virgin coconut oil (VCO) could prevent diclofenac-induced oxidative nephrotoxicity in rats.

Materials and Methods:

Randomized rats were pre-supplemented orally with VCO (5 or 10 ml/kg body weight) from day 1 to 24, and injected with normal saline or diclofenac (100 mg/kg) from day 22 to day 24 intraperitoneally.

Results:

Diclofenac significantly (p<0.05) increased serum urea and creatinine levels. Renal tumor necrosis factor-α (TNF-α) and malondialdehyde (MDA) levels markedly (p<0.05) increased, whereas renal glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) activities considerably (p<0.05) decreased compared to normal control. Histopathological alterations were caused by diclofenac. However, treatment with oral VCO for 21 days prior to diclofenac administration, attenuated histological renal damage, and restored antioxidant enzyme activities and TNF-α levels in kidney.

Conclusion:

These findings revealed that VCO has potential benefits to prevent diclofenac-induced nephrotoxic damage.

Key Words: Diclofenac, Virgin coconut oil, Nephrotoxicity, Antioxidants, Oxidative stress

Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.

Learn More