OBJECTIVE

To study the function of newly cloned NF-kappaB-activating gene (NAP2) and its mechanism. METHEDS: Activation of NF-kappaB and IL-12 promoters as well as modulation of IL-12 synthesis by NAP2 in macrophages were observed by NF-kappaB and IL-12 luciferase reporter assay and intracellular IL-12 staining.

RESULTS

NAP2 could activate NF-kappaB and IL-12 promoter. But IkappaBalphaDN could inhibit their activation. In addition, NAP2 could induce IL-12 expression in macrophage cell line J774.

CONCLUSIONS

NAP2 as a newly cloned NF-kappaB activating gene can activate IL-12 promoter and induce IL-12 expression in macrophages. The data suggest that NAP2 regulates the expression of IL-12 most likely through its modulation of NF-kappaB transcription factor.