Quantitative fluorescence imaging of protoporphyrin IX through determination of tissue optical properties in the spatial frequency domain.
Journal: 2012/May - Journal of Biomedical Optics
ISSN: 1560-2281
Abstract:
The ability to quantitatively determine tissue fluorescence is of interest for the purpose of better understanding the details of photodynamic therapy of skin cancer. In particular, we are interested in quantifying protoporphyrin IX (PpIX) in vivo. We present a method of correcting fluorescence for effects of native tissue absorption and scattering properties in a spatially resolved manner that preserves the resolution of the fluorescence imaging system, based off a homogeneous representation of tissue. Validation was performed using a series of liquid turbid phantoms having varying concentrations of absorber, scatterer, and fluorophore (PpIX). Through the quantification of tissue optical properties via spatial frequency domain imaging, an empirical model based on Monte Carlo simulations was deployed to successfully decouple the effects of absorption and scattering from fluorescence. From this we were able to deduce the concentration of the PpIX to within 0.2 μg/ml of the known concentration. This method was subsequently applied to the determination of PpIX concentration from in vivo normal skin where the model-based correction determined a concentration of 1.6 μg/ml, which is in agreement with literature.
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J Biomed Opt 16(12): 126013

Quantitative fluorescence imaging of protoporphyrin IX through determination of tissue optical properties in the spatial frequency domain

University of California Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612
Modulated Imaging, Inc., 1002 Health Sciences Road, Irvine, California 92612
University of California Irvine, Department of Dermatology, Medical Sciences C-340, Irvine, California 92697-2400
Address all correspondence to: Rolf Saager, University of California Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612; Tel: 949 824 4104; Fax: 949 824 4868; E-mail: ude.icu@regaasr.
Received 2011 Aug 19; Revised 2011 Nov 9; Accepted 2011 Nov 10.

Abstract

The ability to quantitatively determine tissue fluorescence is of interest for the purpose of better understanding the details of photodynamic therapy of skin cancer. In particular, we are interested in quantifying protoporphyrin IX (PpIX) in vivo. We present a method of correcting fluorescence for effects of native tissue absorption and scattering properties in a spatially resolved manner that preserves the resolution of the fluorescence imaging system, based off a homogeneous representation of tissue. Validation was performed using a series of liquid turbid phantoms having varying concentrations of absorber, scatterer, and fluorophore (PpIX). Through the quantification of tissue optical properties via spatial frequency domain imaging, an empirical model based on Monte Carlo simulations was deployed to successfully decouple the effects of absorption and scattering from fluorescence. From this we were able to deduce the concentration of the PpIX to within 0.2 μg/ml of the known concentration. This method was subsequently applied to the determination of PpIX concentration from in vivo normal skin where the model-based correction determined a concentration of 1.6 μg/ml, which is in agreement with literature.

Keywords: fluorescence imaging, turbid media, tissue optics, absorption, scattering
Abstract

Acknowledgments

We acknowledge funding and support from U.S. Army Medical Research, Award No. W81XWH-08-1-0086, Apogen Technologies, Inc., San Diego, California, NIH NCRR Biomedical Technology Research Center (LAMMP: 5P-41RR01192), Beckman Foundation.

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