Photosynthesis in enzymatically isolated leaf cells from the CAM plant Sedum telephium L.
Journal: 2014/January - Planta
ISSN: 0032-0935
A technique has been developed for the enzymatic isolation of leaf cells from the Crassulacean acid-metabolism plant Sedum telephium. The cells exhibited high activity in both (14)CO2 incorporation (30-70 μmol CO2 mg(-1) chlorophyll h(-1)) and O2 evolution in the presence of bicarbonate (60-110 μmol O2 mg(-1) chlorophyll h(-1)). Half-maximum saturation of (14)CO2 incorporation occurred at a bicarbonate concentration of ca. 2 mM (20 μM CO2) at pH 8.4 and 30°C. Two types of light-dependent O2 evolution are reported: O2 evolution in the absence of exogenously supplied bicarbonate (endogenous O2 evolution), and bicarbonate-stimulated O2 evolution. Oxygen evolution in the presence of approximately ambient concentrations of CO2 appeared to be a combination of the endogenous O2 evolution and O2 evolution from fixation of the exogenously supplied CO2.
Similar articles
Articles by the same authors
Discussion board
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.