Nuclear translocation of activated MAP kinase is developmentally regulated in the developing Drosophila eye.
Journal: 2003/September - Development (Cambridge)
ISSN: 0950-1991
PUBMED: 12835387
Abstract:
In proneural groups of cells in the morphogenetic furrow of the developing Drosophila eye phosphorylated mitogen activated protein kinase (MAPK) antigen is held in the cytoplasm for hours. We have developed a reagent to detect nuclear MAPK non-antigenically and report our use of this reagent to confirm that MAPK nuclear translocation is regulated by a second mechanism in addition to phosphorylation. This "cytoplasmic hold" of activated MAPK has not been observed in cell culture systems. We also show that MAPK cytoplasmic hold has an essential function in vivo: if it is overcome, developmental patterning in the furrow is disrupted.
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Development 130(16): 3703-3714

Nuclear translocation of activated MAP kinase is developmentally regulated in the developing <em>Drosophila</em> eye

Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
Present address: Department of Biology, Indiana University, Bloomington, IN 47405, USA
Present address: Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
Author for correspondence (ude.yrome.oibllec@sesomk)

SUMMARY

In proneural groups of cells in the morphogenetic furrow of the developing Drosophila eye phosphorylated mitogen activated protein kinase (MAPK) antigen is held in the cytoplasm for hours. We have developed a reagent to detect nuclear MAPK non-antigenically and report our use of this reagent to confirm that MAPK nuclear translocation is regulated by a second mechanism in addition to phosphorylation. This ‘cytoplasmic hold’ of activated MAPK has not been observed in cell culture systems. We also show that MAPK cytoplasmic hold has an essential function in vivo: if it is overcome, developmental patterning in the furrow is disrupted.

Keywords: MAP kinase, Drosophila, Compound eye
SUMMARY

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