Cell Death Differ 21(7): 1081-1094

PMC: PMC4207476

PMID: 24583642

doi: 10.1038/cdd.2014.27

Notch1 regulates progenitor cell proliferation and differentiation during mouse yolk sac hematopoiesis

Results

Constitutive N1ICD expression in Tie2^{progenitors impairs definitive intraembryonic hematopoiesis}

To study the consequences of Notch1 gain-of-function in hematopoiesis, we used the conditional N1ICD transgenic line R26N1ICD, which upon Cre activation expresses the N1ICD (amino acids 1749–2293) and nuclear-localized enhanced GFP.³²Tie2-Cre;N1ICD embryos that constitutively expressed N1ICD and EGFP in Tie2^{hematovascular progenitor cells died at E11.0.}³³ At E9.5 (Figures 1a–d), transgenic embryos were smaller than wild-type (WT) littermates and the YS was pale and lacked well-formed blood vessels (Figure 1b). Similarly, although the dorsal aorta and the umbilical and vitelline arteries were preserved, the intraembryonic P-Sp/AGM area lacked hemoglobinized cells (Figure 1d). In most Tie2-Cre;N1ICD embryos, the YS contained small, randomly located concentrations of hemoglobinized red blood cells (Figure 1b). This phenotype suggested a severe alteration in embryonic angiogenesis associated with defects in primitive or definitive hematopoiesis. The phenotype worsened by E10.5 (Figures 1e–h) and was accompanied by severe cardiac defects that presumably resulted in hemodynamic alterations, causing embryonic death.³³

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Figure 1

Constitutive Notch1 activation on Tie2^{progenitor cells impairs hematopoietic development. WT and}Tie2-Cre;N1ICD embryos (left and right panels, respectively) at E9.5 (a–d) and E10.5 (e–h), with the YS (a, b, e and f) and without it (c, d, g and h). (a, b) Morphology of the YS of WT and Tie2-Cre;N1ICD embryos. WT YS contains normally developed blood vessels (black arrow in a) that are absent in transgenic YS. Tie2-Cre;N1ICD YS is very pale, with hemoglobinized cells concentrated at one pole (white arrow in b). (c, d) Lateral views of WT and Tie2-Cre;N1ICD embryos. Black arrows indicate the P-Sp/AGM region with hemoglobinized content in WT, and the pale region in the transgenic embryo, indicative of defective definitive hematopoietic development. (e–h) At E10.5, defects in Tie2-Cre;N1ICD YS and embryos are more severe. Black arrows in (e) mark the well-developed WT YS blood vessels that are never seen in transgenic YS (f), which instead contains randomly located pockets of high hemoglobin content (white arrow). Lateral views of WT (g) and Tie2-Cre;N1ICD (h) embryos show the hemoglobinized dorsal aorta in the AGM region (black arrows) of a WT embryo that is absent in the transgenic embryo. Scale bars: 800 μm

CD31/PECAM1 was expressed on hematopoietic and endothelial cells in the P-Sp/AGM dorsal aorta and the YS vitelline artery (Supplementary Figure 1a–j), indicating that early endothelial differentiation occurs in these vessels. Nevertheless, the arterial marker Efnb2 was ectopically expressed in venous endothelium (Supplementary Figure 1k and l) while the venous marker Ephb4 was not expressed (Supplementary Figure 1m and n). N1ICD staining labels arterial endothelium (Supplementary Figure 1o and Del Monte et al.³⁰), so the observation that Tie2-Cre-driven ectopic N1ICD expression promotes arterial differentiation of venous endothelium is consistent with the requirement of Notch in arterial endothelium differentiation.³⁴ LacZ staining of Tie2-Cre;R26R embryos (Supplementary Figure 1p) indicates that this driver is active throughout the endothelium and circulating cells and is an excellent tool for studying the effect of Notch overexpression in these tissues.

Histological analysis of embryos and YS revealed that, compared with the WT extraembryonic vasculature formed by the capillary plexus (Figure 2a) and vessels full of circulating cells (Figure 2c), extraembryonic vessels in Tie2-Cre;N1ICD embryos were dilated and expanded (Figure 2b). Moreover, transgenic embryos exhibited two types of blood islands: some with the lumen almost empty (Figure 2d) and others full of cells (Figure 2b). These presumably corresponded to the hemoglobinized YS pockets described above (Figures 1b and f).

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Figure 2

Tie2-Cre;N1ICD embryos display severe vascular alterations and a blockade of intraembryonic definitive hematopoiesis. (a–d) Bright-field images of H&E-stained transverse sections of YS from E9.5 WT (a, c) and Tie2-Cre;N1ICD (b, d) embryos, showing the differences in blood vessel diameter and circulating cell number. Transgenic YS (b) exhibits two types of blood island, with the lumen empty (black arrowhead) or with a high concentration of circulating cells (white arrowhead). Panels (c) and (d) show detailed views of a normal blood vessel in WT YS (c) and a dilated vessel of Tie2-Cre;N1ICD YS (d). (c′, d′) Confocal image of a WT (c′) and Tie2-Cre;N1ICD YS (d′) stained with DAPI. The white arrowheads in point to the circulating cells in WT YS that do not express EGFP (c′), and to EGFP^{coexisting with EGFP}^{circulating cells in the transgenic YS (}d′). (e, f) Transverse sections of E9.5 dorsal aorta at the P-Sp/AGM region level in WT (e) and Tie2-Cre;N1ICD embryos (f); transgenic animals have bilateral dorsal aortas (white arrows) and lack the hematopoietic cell clusters that start to bud off from the endothelium in WT embryos (black arrows in e). G, gut. (g, h) In situ hybridization of AML1/Runx1 in WT (g) and Tie2-Cre;N1ICD (h) vitelline artery. Hematopoietic cells almost obliterate the lumen of WT vitelline artery, whereas a single small hematopoietic cell cluster is seen in the Tie2-Cre;N1ICD vessel (black arrow in h). (i) Dot plots showing a marked reduction in the CD45^{cell population in the caudal portion of}Tie2-Cre;N1ICD (right) versus WT (left) embryos. Data show frequency (mean±S.E.M., N=3). Scale bars: 25 μm

Confocal analysis of WT (Figure 2c′) and transgenic YS showed that EGFP expression labeled most although not all circulating cells within the vessels, indicating that Tie2-Cre-driven ectopic N1ICD expression was not uniform (Figure 2d′). At E9.5, the Tie2-Cre;N1ICD dorsal aorta was still a bilateral artery (Figure 2f) indicating a delay in vascular development. Unlike in the WT situation, where hematopoietic cell clusters were observed budding from the ventral wall of the dorsal aorta (Figure 2e), no clusters were detected in Tie2-Cre;N1ICD dorsal aorta (Figure 2f) or umbilical and vitelline arteries (data not shown). Nevertheless, the vitelline artery of 10% of the embryos contained cell clusters populated by hematopoietic progenitor cells, demonstrated by the normal expression of AML1/Runx1 mRNA (Figures 2g and h).

These results suggest that constitutive Notch1 activation promotes an early blockade in the generation of definitive hematopoietic cell clusters. Supporting this, flow cytometry analysis detected 8.4-fold fewer circulating EGFP^CD45^{cells in E9.5}Tie2-Cre;N1ICD embryos (Supplementary Figure 2a). In the same embryos, the intraembryonic CD45^{population was almost absent in the caudal region (which includes the P-Sp/AGM) (}Figure 2i). These data suggest that constitutive Notch1 expression in Tie2^{cells leads to defects in YS hematopoietic and vascular development and to a blockade of intraembryonic definitive hematopoiesis similar to that described for Notch-deficient mice.}²⁶, 35, 36, 37

N1ICD expression reduces the hematopoietic potential and favors the endothelial phenotype of Tie2^{cells in the YS}

Flow cytometry analysis of Tie2-Cre;N1ICD YS cells at E9.5 showed that just 40–60% of the recovered cells were EGFP^{, which may suggest incomplete Cre-mediated recombination and activation of the EGFP transgene (}Figure 3a). The number of EGFP^{and EGFP}^{cells recovered from YS of}Tie2-Cre;N1ICD mice was lower than that recovered from WT mice (Figure 3b; Supplementary Figure 2b). The co-expression of EGFP and N1ICD in Tie2-Cre;N1ICD mice³³ allowed us to examine the hematopoietic potential of FACS-purified total EGFP^{YS cell populations and of EGFP}^{cells expressing the stem cell factor (SCF) receptor (c-Kit) at E9.5. After 7 days in methylcellulose cultures, the numbers of colonies derived from E9.5 EGFP}^{cells, both total and c-Kit positive, was 10- to 20-fold lower than those derived from WT cells (}Figure 3c); however, numbers of primitive erythroid colonies were normal at E8.5 in total YS EGFP^{and WT cells (}Supplementary Figure 2c). In addition, whereas colonies growing from E9.5 WT and EGFP^{cultures were BFU-E and CFU-M (}Figure 3d, left panel and not shown), the small number of colonies detected in EGFP^{cultures contained elongated fibroblast-like cells surrounding central clusters of small round cells (}Figure 3d, right panel). When EGFP^{colonies were transferred to fibronectin-coated plates, tube-like structures developed after 48 h that appeared to be pseudo-endothelial cords (}Figure 3e). These cells were positive for the endothelial-specific marker von Willebrand factor (vWF) (Figure 3f). In agreement with these results, the CD31^CD41^CD45^Mac1^TER119^{hemogenic endothelial cells}³⁸ were significantly increased in EGFP^{cells from}Tie2-Cre;N1ICD YS overexpressing Notch1 at E9.5 (Figures 3g and h).

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Figure 3

Immature progenitor cell populations in E9.5 Tie2-Cre;N1ICD mice have a weak hematopoietic potential and maintain endothelial progenitors. (a) Contribution of EGFP^{and EGFP}^{(N1ICD transgene-expressing) cells to YS, blood and P-Sp in E9.5}Tie2-Cre;N1ICD embryos. The graph shows the relative percentage of EGFP^{and EGFP}^{cells in preparations of the indicated cell suspensions (mean±S.E.M.,}N=13 for YS and P-Sp and N=7 for blood). (b) N1ICD expression in Tie2-Cre;N1ICD YS reduces the numbers of cells expressing and not expressing the transgene at E9.5. Bar chart shows quantification of YS cells in WT and in Tie2-Cre;N1ICD EGFP^{and EGFP}^{cell populations. Bars represent mean±S.E.M. (}N=15). (c) Hematopoietic potential of WT and Tie2-Cre;N1ICD YS cells. Cells were cultured in MethoCult3434 medium for 7 days. Graphs show the absolute numbers of BFU-E and CFU-M (erythroid and myeloid, respectively) per 100 cells, detected in cultures of total cells at E9.5 and E10.5 (left) or from FACS-purified c-Kit-expressing progenitor cells of WT and EGFP^{and EGFP}Tie2-Cre;N1ICD cells at E9.5 (right). Data are mean±S.E.M. (N=3). (d) Microphotographs showing representative colonies of WT and EGFP^{cells. The periphery of EGFP}^{colonies is populated by elongated, polarized cells. (}e) Pseudo-endothelial structures formed after in vitro culture of EGFP^{CFUs on fibronectin-coated plates in the presence of the pro-endothelial factors bFGF and VEGF. (}f) Merged microphotographs of EGFP^{CFU cells stained with vWF antibody (left) or with an isotype control antibody (right). (}g) Representative cytometry plots of E9.5 YS cells from WT and Tie2-Cre;N1ICD mice stained for CD41, CD31, CD45, TER119 and CD11b/Mac1. CD41^CD45^TER119^CD11b^{cells were electronically excluded, and CD31}^{is plotted showing green channel fluorescence to identify EGFP}^{cells. Boxed areas define hemogenic EC cells as CD31}^CD41^CD45^TER119^CD11b^{. Numbers are percentages of gated cells, as mean±S.E.M, (}N=4). (i) Representative cytometry plots of E9.5 YS cells from WT and Tie2-Cre;N1ICD mice stained for c-Kit, and plotted against green fluorescence channel. Boxes define c-Kit^{and c-Kit}^{cell populations, and numbers are percentages of gated cells, as mean±S.E.M. (}N=25). (h, j) The graphs show the number of cells of each population per YS; bars are mean±S.E.M,; data were calculated from the total YS cell counts presented in (b). Bars are labeled as in (b). **P<0.01, ***P<0.001

In the WT YS, two cell populations could be distinguished ex vivo according to the levels of c-Kit, c-Kit^{cells, corresponding to immature progenitor cells, and c-Kit}^{cells, associated with more mature cellular stages.}³⁹ The relative size of the c-Kit^{population was larger in YS cells from EGFP}Tie2-Cre;N1ICD embryos (Figures 3i and j), whereas that of the c-Kit^{cells was similar. Interestingly, some of EGFP}^c-Kit^{cells maintained the expression of Tie2 whereas WT c-Kit}^{cells and EGFP}^{cells did at very low levels, and more than half of them did not express CD31 or KDR (}Supplementary Figure 3a and b). In absolute terms, EGFP^{cell suspensions contained 1.5-fold more c-Kit}^{cells than WT cell suspensions, whereas immature c-Kit}^{cells were in fact less abundant in}Tie2-Cre;N1ICD mice (0.6-fold lower than WT). EGFP^{cells in}Tie2-Cre;N1ICD YS (Figure 3a) could in principle serve as an internal control for the effects of Notch1. However, both the numbers of EGFP^c-Kit^/^{cells and the CFUs obtained from them (}Figures 3c and j) were markedly reduced with respect to cells and colonies derived from WT embryos, indicating that these EGFP^{cells were affected by intracellular Notch1 expression in EGFP}^cells.

Erythroid and megakaryocyte development is blocked in Tie2-Cre;N1ICD mice

Pellets obtained by centrifugation of WT YS were reddish, whereas those from Tie2-Cre;N1ICD YS were pale (Figure 4a), suggestive of an abnormal erythropoiesis. Immunostaining of YS cells for c-Kit, CD71 and TER119 allows identification of several differentiation stages in the erythroid developmental pathway. The proerythroblast stage in mouse BM includes large cells that co-express c-Kit, CD71 and low levels of the erythroid-specific marker TER119.⁴⁰ Analysis of the expression of CD71 and TER119 in WT and EGFP^{cells at E9.5 revealed markedly lower numbers of basophilic CD71}^TER119^{erythroblasts in}Tie2-Cre;N1ICD mice (R2), accompanied by an accumulation of less mature CD71^TER119^{proerythroblasts (R1) (}Figure 4b). This block in the differentiation of TER119^{cells was observed already at E8.5 (}Supplementary Figure 2d), indicating that Notch1 overexpression affects also the primitive erythropoiesis wave. Cytospin preparations from total WT and EGFPTie2-Cre;N1ICD cells showed that N1ICD-expressing cells had a high nucleocytoplasmic ratio and a more acidophilic cytoplasm (Figure 4c). Among the EGFP^{cells we observed an immature c-Kit}^TER119^{population not seen in WT cells (}Figure 4d), showing that part of the c-Kit^{population described in}Figure 3j is TER119 positive and belongs to the erythroid lineage. The intensity of the CD41/TER119 expression allows the discrimination of primitive and definitive erythroid populations. Most of the erythroid cells found in YS at E9.5 are dim for CD41 and TER119 (Figure 4e), therefore corresponding to the primitive wave, as has been described.¹⁵ However, EGFPTie2-Cre;N1ICD cells displayed lower levels of TER119 than WT cells. Accordingly, the expression of both the fetal βH1 and the adult-type β1 hemoglobin was highly diminished in EGFPTie2-Cre;N1ICD cells compared with WT cells (Figure 4f). Finally, the quantification of the more immature c-Kit^CD41^CD45^Mac1^TER119^{common erythro-myeloid progenitors}⁴¹ by 6-color cytometry showed that they were significantly increased in EGFP^{cells compared with WT cells (}Figure 4g). In summary, constitutive N1ICD expression on Tie2^{cells blocks in the YS the maturation of erythroid precursors both of the primitive erythropoiesis wave and of the definitive transient erythro-myelopoiesis, resulting in an accumulation of erythro-myeloid progenitors and of immature proerythroblasts. The EGFP}^{erythroid cells present in}Tie2-Cre;N1ICD YS had phenotypes similar to WT cells, although their total numbers were reduced (Supplementary Figure 4a and b). The expression levels of adult-type β1 hemoglobin were strongly reduced, whereas the levels of fetal βH in EGFP^{cells were lower compared with WT cells, this may be related with the smaller size of the EGFP}^{erythroid cell compartment (}Figure 4f).

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Figure 4

Abnormal erythroid and megakaryocyte differentiation in Tie2-Cre;N1ICD YS. (a) YS cells centrifuged on 96-well plates, revealing the low hemoglobin content of Tie2-Cre;N1ICD YS, indicating defective erythroid differentiation. (b) Representative dot plots of double-stained WT and electronically gated EGFP^{cells for the erythroid marker TER119 and the transferrin receptor CD71. Boxed areas define CD71}^TER119^{(R1) and CD71}^/TER119^{(R2) cells. The graph in the right shows the relative percentage of R1 and R2 cell populations (mean±S.E.M.;}N=6) in WT and electronically gated EGFP^{and EGFP}^{cells. (}c) Microphotographs of May Grümwald-Giemsa staining in WT and FACS-purified EGFPTie2-Cre;N1ICD cells. (d) Representative dot plots of WT and electronically gated EGFP^{cells for TER119 and c-Kit. Numbers are relative percentages of the TER119}^c-Kit^{, cells boxed (mean±S.E.M.;}N=6). The right graph shows the quantification of numbers of TER119^c-Kit^{cells/YS calculated as in}Figure 3j (mean±S.E.M.; N=6) for WT and electronically gated EGFP^{and EGFP}^{cells. (}e) The graph represents the mean fluorescence intensity (MFI) data obtained from E9.5 WT and electronically gated EGFP^{and EGFP}^{cells from}Tie2-Cre;N1ICD mice stained for TER119, as mean±S.E.M. (N=4). Staining of blood cells at E11.5 is shown as a control. (f) Quantifications of embryo-derived primitive (βH1) and definitive (β1) hemoglobins are shown in the bar graph. WT and FACS-purified EGFP^{and EGFP}Tie2-Cre;N1ICD cells were submitted to RNA extraction, cDNA was synthesized and RT-qPCR was performed as indicated in Materials and Methods. The Bio-Rad CFX Manager software was used to calculate the C_T of each reaction, and the relative amount of specific cDNA in each sample was determined by the ΔC_T method. Results are displayed as the relative expression of each transcript over Gα gene expression (mean±S.E.M.; N=4). (g) Representative FACS analysis of WT and Tie2-Cre;N1ICD mice stained for TER119, CD45/Mac1, CD41 and c-Kit. TER119^CD45/Mac1^{cells were gated (left dot plot) and analyzed for CD41 and c-Kit, as indicated in the plots from WT and electronically gated EGFP}^{cells. The boxes identify the c-Kit}^CD41^{erythro-myeloid progenitor (EMP) population; numbers are percentages (mean±S.E.M.;}N=4). The right graph shows the EMP quantification/YS calculated as in Figure 3j (mean±S.E.M.; N=4) for WT and electronically gated EGFP^{and EGFP}^{cells. (}h) Representative FACS analysis of WT and electronically gated EGFP^{cells stained for CD9 and CD41. Boxed areas define the double-positive CD9}^CD41^{megakaryocytic population and the CD9}^CD41^{cell population. The graphs in the right show the relative percentages and absolute numbers of both cell populations in WT and electronically gated EGFP}^{and EGFP}Tie2-Cre;N1ICD YS (N=5). (i) Left panel, Representative graph of the relative percentage of megakaryocyte progenitors (MK-CFUs) present in FACS-purified CD41^{cells from WT}versus EGFP^{populations (}N=3). Right panel, Relative percentages of MK-CFUs expressing acetylthiocholiniodide (AcH) in WT versus EGFP^{cells (}N=3). (j) Microphotographs showing cultures of CD9^CD41^{FACS-purified cells from E9.5 WT and EGFP}Tie2-Cre;N1ICD YS cells at 48 h of culture. *P<0.05, **P<0.01, ***P<0.001

Besides erythroid cells, the first hematopoietic-lineage cells generated in the YS belong to the megakaryocyte (MK) lineage.¹⁵, 42 WT and Tie2-Cre;N1ICD YS did not differ in relative numbers of CD41^CD9^{cells, which correspond to embryonic MKs (}Figure 4h).⁴² Nevertheless, fewer total EGFP^{and EGFP}^CD41^CD9^{MKs were present in}Tie2-Cre;N1ICD YS compared with WT YS. Purified EGFP^CD41^CD9^{cells cultured in semisolid MK-specific medium produced fewer MK-CFUs than their WT counterparts, and 50% of them contained immature acetylthiocholiniodide}^{cells (}Figure 4i). After 24 h in liquid culture, purified CD41^CD9^{cells from WT and}Tie2-Cre;N1ICD embryos began to differentiate to MKs, exhibiting cytoplasmic prolongations. However, only WT MKs developed into pro-platelets at 48 h, whereas EGFP^{MKs disappeared from the cultures (}Figure 4j). Thus, regardless of the enrichment in erythro-myeloid progenitors and the relatively normal numbers of CD41^CD9^{MK progenitors in the transgenic YS, their differentiation}in vitro is defective and MK progenitors die before reaching their final maturation stage.

Phenotypic characterization of myeloid immature progenitor cell populations in WT and Tie2-Cre;N1ICD YS at E9.5

To characterize the myeloid differentiation potential of WT and Tie2-Cre;N1ICD YS cells at E9.5, we analyzed the co-expression of c-Kit and CD45, which allowed us to trace the appearance of myeloid CD45^{cells in the YS.}³⁹ Similar numbers of EGFP^CD45^{cells and WT CD45}^{cells were detected (}Figure 5a). However, the EGFP^CD45^{population from}Tie2-Cre;N1ICD YS contained a smaller immature c-Kit^CD45^{(R1) fraction (}Figure 5b). In contrast, the numbers of c-Kit^{cells with higher CD45 levels (R2, c-Kit}^CD45^{) remained similar in WT and EGFP}^{YS. The more mature intermediate c-Kit}^CD45^{(R3) cells, showing loss of c-Kit, accumulated in EGFP}^{YS, whereas the numbers of more mature c-Kit}^CD45^{cells (R4) were similar in the two genotypes (}Figure 5b). Expression analysis of the macrophage marker F4/80⁴³ detected similar numbers of F4/80^{cells in WT samples and EGFP}^{cells from}Tie2-Cre;N1ICD YS (Figure 5c). However, the WT F4/80^{YS population contained a subpopulation of CD45}^{cells expressing the myeloid marker Mac1 (Mac1}^CD45^{) that was absent in F4/80}^EGFP^{cells (}Figure 5c); moreover, Mac1^{cells were found only in WT YS (}Figure 5d), whereas the EGFP^{YS population contained elevated numbers of immature Mac1}^{cells. Mac1}^{cells in WT YS are mature myeloid cells, since they were negative for c-Kit, and expressed Gr1 and high levels of CD45 (}Figures 5d and e). Thus, N1ICD expression on Tie2^{cells does not affect the initiation of myelopoiesis, but impairs developmental progression to the final maturation stages, and consequently causes accumulation of intermediate c-Kit}^CD45^{stages. The myeloid differentiation potential of EGFP}Tie2-Cre;N1ICD YS cells was also altered, as reflected by the smaller numbers of EGFP^CD45^{cells and of EGFP}^F4/80^{cells and by the lower percentages of immature myeloid cellular stages (R1 and R2;}Figure 5b). Interestingly, the fact that fractions of more mature Mac1^Gr1^{cells were detected at similar level in EGFP}Tie2-Cre;N1ICD YS cells than in WT preparations, suggested that EGFP^{cells were able to reach final myeloid development stages (}Supplementary Figure 4d and e).

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Figure 5

N1ICD inhibits the differentiation of YS myeloid progenitors. Total WT and Tie2-Cre;N1ICD YS cells were stained with the indicated antibodies and analyzed by flow cytometry. (a) CD45^{cell numbers are elevated in YS from}Tie2-Cre;N1ICD mice at E9.5. Dot plots show a representative detection of myeloid CD45^{cells (boxed areas) in WT and}Tie2-Cre;N1ICD preparations. Inside the plots cell percentages are shown as mean±S.E.M., N=15. The bar chart shows the number of CD45^{cells per YS, calculated for each sample using the corresponding data presented in}Figure 3b. (b) Dot plots of WT and electronically gated EGFP^{YS cells stained for the hematopoietic markers CD45 and c-Kit. Gates define the following subpopulations: c-Kit}^CD45^{(R1), c-Kit}^CD45^{(R2), c-Kit}^CD45^{(R3) and c-Kit}^CD45^{(R4). The bar chart shows relative percentages of electronically gated WT and EGFP}^{and EGFP}Tie2-Cre;N1ICD YS cells as mean±S.E.M., N=10. (c) Upper panel: dot plots show a representative detection of the macrophage marker F4/80, boxed areas with relative percentages as mean (±S.E.M., N=4), in total WT and Tie2-Cre;N1ICD YS cells, detected in the green fluorescence channel to identify EGFP^cells.Lower panel: dot plots show Mac1 and CD45 expression in electronically gated WT F4/80^{cells and EGFP}^F4/80^{cells. The boxed areas highlight a Mac1}^CD45^{subpopulation not found among EGFP}^F4/80^{cells. (}d) Mac1 antibody staining in total WT and Tie2-Cre;N1ICD YS cells, displayed as in (c). The boxes mark the Mac1^{and Mac1}^{subpopulations, with relative numbers shown to the right as mean±S.E.M.,}N=4; Mac1^{cells are absent in EGFP}^{transgenic mice. The lower histograms show c-Kit-stained cells in the electronically gated Mac1}^{and Mac1}^{subpopulations from WT or EGFP}^{YS. Mac1}^{cells in WT (solid line) and EGFP}^{(open line) YS cells (left histogram) co-express c-Kit. WT Mac1}^{cells do not express c-Kit, indicating their more mature phenotype (right histogram). (}e) Dot plots of Gr1 staining (boxed areas) showing the absence of Gr1^{cells in the EGFP}Tie2-Cre;N1ICD YS cells. The percentage of Gr1^{cells is indicated as mean±S.E.M.,}N=3. The contour plot (bottom) shows co-expression of Mac1 and CD45 in electronically gated WT Gr1^{cells. (}f) Quantitative PCR analysis of FACS-purified WT and EGFP^{cells for the expression of selected genes implicated in early hematopoiesis (}SCL, Fli1, GATA3, Ldb1, Lmo2 and Runx1), myeloid development (GATA2, PU.1, c-fms, Mpo and LysM) and erythroid development (GATA1, KLF1 and KLF2). Data are shown as the fold change in mRNA expression level (ΔΔCt method) between EGFP^{cells from}Tie2-Cre;N1ICD YS relative to WT YS cells. Bars are mean±S.E.M., N=3. *P<0.05, **P<0.01, ***P<0.001

Notch1 activation on Tie2-derived YS cells dysregulates the transcriptional program that initiates hematopoietic differentiation

Analysis of FACS-purified EGFP^{and WT YS cells by quantitative PCR (qPCR) revealed an association of N1ICD expression with substantial changes in expression of hematopoietic and vasculo/endothelial genes. EGFP}^{cells expressed higher levels of}Runx1 (Figure 5f). The transcription factors SCL, Fli1 and Gata family members are implicated in the earliest stages of hematopoiesis/vasculogenesis in the mouse embryo, and have been proposed to form a gene regulatory network that operates during specification of vasculo-hematopoiesis.⁴⁴ EGFP^{cells expressed lower levels of}Scl, Gata3 and Fli1 transcripts, whereas Gata1 and Gata2 were upregulated (Figure 5f). The transcript levels of Pu1 and c-fms, which have a critical role in differentiation to myeloid lineages,⁴⁵ were increased in EGFP^{cells. In contrast, transcripts encoding myeloperoxidase and lysozyme, involved in differentiation of more mature myeloid stages, were strongly downregulated, in agreement with the incomplete myeloid development observed in EGFP}Tie2-Cre;N1ICD cells.⁴³ Despite the marked alteration in erythropoiesis observed in Tie2-Cre;N1ICD embryos, the transcription of Klf1 and Klf2, important genes in this process⁴⁶ did not show variations. Constitutive Notch1 activation in Tie2-derived cells thus dysregulates the transcriptional program that initiates the first differentiation steps in early hematopoietic progenitor cells and some lineage-restricted precursors.

N1ICD expression in Tie2^{cells induces high proliferation, cell-cycle activation and reduced apoptosis}

Notch1 activation has been shown to enhance self-renewal, increases the stem-cell pool, alters differentiation processes and promotes cell-cycle progression.²⁴ The erythroid/myeloid differentiation arrest that we found at E9.5 in EGFP^{cells derived from Tie2}^{N1ICD expressing progenitors was accompanied by an increase in the number of erythroid/myeloid c-Kit}^{immature cell progenitors. Also, FACS-purified EGFP}Tie2-Cre;N1ICD cells cultured on OP9 grew at a 10-fold higher rate than WT or EGFPTie2-Cre;N1ICD cells (Figure 6a). Analysis of the cell-cycle status of purified EGFP^{cells by propidium iodide (PI) staining revealed a higher number of cells in the S/G}₂M phases compared with WT cells (Figure 6b). This was confirmed also for WT and Tie2-Cre;N1ICD cells by Hoechst staining of the nuclei of live cells, which allows ex vivo identification cell-cycle status, revealing a higher proportion of EGFP^{cells in S phase (}Figure 6c) than WT or EGFPTie2-Cre;N1ICD cells. In contrast, in vivo incorporation of the nucleotide analog EdU did not differ between EGFP^{and WT cells (}Figure 6d). To exclude the influence of gestational age differences between mice, we compared EdU incorporation between EGFP^{and EGFP}^{cells in the same}Tie2-Cre;N1ICD embryos, and found that cells expressing the transgene incorporated more EdU than EGFP^{cells. Apoptotic cells, defined as those in the ≤G}₀ region and by the expression of Annexin-V, were less abundant in total EGFP^{cells than in total WT cells or in EGFP}Tie2-Cre;N1ICD cells (Figure 6e). These data thus indicate that N1ICD expression in Tie2^{cells prevents apoptosis and induces a highly proliferative state in most YS hematopoietic progenitors, with an accumulation of cells in the S phase, which likely interferes with their differentiation.}

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Figure 6

Proliferation, cell cycle and apoptosis in Tie2-Cre;N1ICD YS. (a) Equal numbers (2 × 10^{cells/well) of WT or}Tie2-Cre;N1ICD YS cells were cultured on OP9 stromal cells. Cells were recovered after 7 days, counted, replated at 2 × 10^{cells/well and cultured for further 7 days. The percentage of EGFP}^{cells was detected by flow cytometry, and absolute numbers of each cell type were calculated from the total number of recovered cells. Fold increase in cell number was calculated at 7 and 14 days. Mean±S.E.M.,}N=3. (b) Representative dot plots of FACS-purified WT and EGFPTie2-Cre;N1ICD cells, showing cell size (SSC) and propidium iodide staining for cell-cycle analysis. Boxed areas define G_o/G₁ phase (left box) and the S plus G₂M phase populations (right box), and numbers are the percentages in this experiment (N=2). (c) DNA content quantified by cytometric analysis of electronically gated EGFPTie2-Cre;N1ICD and WT cells stained with Hoechst 33342. Representative histograms are shown for WT, EGFP^{and EGFP}^{YS cell populations. Vertical dotted lines indicate the cell-cycle phases defined by the control cells used (adult thymus). The bar chart shows cells in G}₀/G₁, S and G₂M as a percentage of total cells found in cycle (>G₀) (N=4). (d) Relative percentage of EGFP^{, EGFP}^{and WT cells that incorporated the nucleotide-analog EdU 1 h after intraperitoneal injection into the pregnant females (}N=6 for cells purified from YS of Tie2-Cre;N1ICD mice and N=11 for WT YS cells). (e) Left panel, Percentages of apoptotic cells (<G₀ cell population) detected in the DNA content cytometry analysis shown in (c) in WT and EGFP^{YS cells.}Right panel, Percentages of apoptotic WT and EGFP^{and EGFP}^{YS cells defined by cytometric analysis of staining with Annexin V and 7-aminoactinomycin D (7AAD) (}N=4). (f) Fold change (ΔΔCt method) in mRNA expression levels of Notch pathway and cell-cycle regulatory genes in FACS-purified EGFPTie2-Cre;N1ICD cells relative to WT cells. The data were obtained as in Figure 5f for each sample. *P<0.05, **P<0.01, ***P<0.001

Analysis of Notch pathway genes by qPCR revealed that Notch1, Hey2, Hes1, Jag1, Jag2, Dll1 and Dll4 were upregulated in EGFP^{cells relative to WT cells, consistent with an increase in Notch signaling activity (}Figure 6f). Interestingly, EGFPTie2-Cre;N1ICD cells showed equivalent levels to those from WT cells, except for Jag2, which was upregulated. Among the regulators of the cell-cycle machinery, both Cyclin-D1 and c-myb were induced in EGFP^{cells relative to WT cells, whereas the cell-cycle inhibitor}p27 was downregulated. No significant changes were observed for c-myc, Id/Atp8b2 or p21 (Supplementary Figure 4f).

Constitutive N1ICD expression in Tie2^{progenitors impairs definitive intraembryonic hematopoiesis}

Figure 1

Figure 2

N1ICD expression reduces the hematopoietic potential and favors the endothelial phenotype of Tie2^{cells in the YS}

Figure 3

Erythroid and megakaryocyte development is blocked in Tie2-Cre;N1ICD mice

Figure 4

Phenotypic characterization of myeloid immature progenitor cell populations in WT and Tie2-Cre;N1ICD YS at E9.5

Figure 5

Notch1 activation on Tie2-derived YS cells dysregulates the transcriptional program that initiates hematopoietic differentiation

N1ICD expression in Tie2^{cells induces high proliferation, cell-cycle activation and reduced apoptosis}

Figure 6

Discussion

The aim of this study was to determine whether Notch function in embryonic hematopoiesis is restricted to the definitive hematopoietic compartment, as has been proposed based on loss-of-function approaches.⁴⁷ The Tie2 promoter is active in endothelial and hematopoietic lineages from E7.5 onwards,⁴⁸ and almost all primitive hematopoietic cells can be traced to Tie2^{cells. N1ICD and Tie2 have a similar expression pattern in YS blood islands.}²⁹, 30 As a strategy to induce Notch1 in embryonic hematopoietic cells we generated double transgenic embryos constitutively expressing N1ICD in Tie2^{hematovascular progenitor cells. Our results show that constitutive N1ICD expression in Tie2}^{hematopoietic progenitors stimulates cellular proliferation at the expense of differentiation, disturbing the delicate balance between both processes and leading to a block of hematopoietic differentiation while promoting the accumulation of cells of intermediate phenotypes (}Figure 7).

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Figure 7

Yolk sac hematopoiesis alterations in Tie2-Cre;N1ICD embryos. In the mouse at around embryonic day (E) 7.0–7.25 early hemangioblasts (HG) emerged from the posterior streak mesoderm and give rise to hemogenic endothelial cells (hEC), and angioblasts (AG) progenitor cells on the extraembryonic YS blood island. The AG will produce part of the YS vascular endothelial cells (EC). On the other hand, YS hematopoiesis occurs in two waves, the primitive (from ∼E7.25, yellow box) and definitive (green box) waves that overlap, temporally and spatially, and produce relatively short-lived cells. hEC give rise, first, to bipotential primitive megakaryocyte/erythroid progenitor (pMEP) that will in turn generate specific primitive progenitors for both cell lineages (pMK-CFC and EryP-CFC); and second, to primitive macrophage precursor cells (Mac-CFC). These progenitors will generate the primitive hematopoietic cell lineages: primitive erythrocytes (EryP), primitive megakaryocytes (pMK) – able to give rise to proplatelets – and primitive macrophages (pMΦ). This short-lived primitive wave is rapidly followed by the second hematopoietic wave that comprises YS definitive erythroid, megakaryocyte and several myeloid lineages. The YS definitive populations are believed to arise from transient multipotent progenitors (tMPPs) – but not from hematopoietic stem cells, which emerge in the mouse YS at around E9.0 – that are detected from ∼E8.25 and generate different transient cell populations of restricted erythro-myeloid progenitors (EMPs). EMPs comprise definitive-type bipotential megakaryocyte/erythroid progenitor (dMEP) that will give rise to transient definitive megakaryocyte and erythrocyte progenitors (dMK-CFC and BFU-E) and definitive myeloid progenitors (Mye-CFC), from which definitive-type erythrocytes (EryD), megakaryocytes (dMK) – with the capacity to originate proplatelets – and several myeloid lineages: macrophages (MΦ), granulocytes and mast cells will be produced. The expression of N1ICD in early Tie2^{progenitors produces severe alterations in both YS}^{primitive and definitive hematopoiesis characterized by high frequency of c-Kit-expressing multipotential and lineage-restricted progenitors (encircled by the orange bubbles) at E9.5, whereas it blocks the generation of the last maturation stages of erythrocytes, megakaryocytes and myeloid lineages. First, no CD71}^TER119^{and Mac1}^F4/80^{cells are produced. In contrast, lineage-restricted progenitors accumulate in the initial or intermediate stages of their differentiation (CD71}^TER119^{proerythroblasts and Mac1}^F4/80^{myeloid progenitors). Accordingly, diminished numbers of CD9}^CD41^{megakaryoblasts are found among EGFP}^{YS cells and the final steps of differentiation}in vitro are selectively blocked. On the other hand, ectopic N1ICD expression in these intermediate progenitors (delimited by green open lines) induces misbalanced proliferation and apoptosis, preventing their normal differentiation. Thick blue arrows denote an increase in cell frequency; weak red arrows indicate a restriction on cell frequency

It is well established that Tie2 is required for primitive and definitive hematopoiesis.⁴⁹ We have observed that Tie2-Cre;N1ICD mice show around 50% of EGFP^{cells, indicating that}Tie2-Cre-driven recombination is, as previously reported,⁵⁰ incomplete. EGFP^{cells express low fractions of Tie2, similarly to WT cells, and although in EGFP}^{cells the expression of Notch1 and related genes is highly increased, in EGFP}^{cells it is close to that from WT, except for}Jag2. Therefore, although we cannot completely exclude that some EGFP^{cells derive from rare Tie2-independent hematopoietic cells, they are very likely the result of incomplete Cre recombination. Our data on EGFP}^{cells provide evidence of a non-cell autonomous effect of Notch1, probably acting through the}Jag ligand. In fact, EGFP^{cells have reduced numbers and seem to have altered c-Kit}^{progenitor cells and differentiation}in vitro. It seems that, in vivo, once the c-Kit^{cells overcome their differentiation block, they are able to proceed to normal primitive erythro-myelopoiesis (erythroid/MK differentiation, and CD45}^Mac1^Gr1^{myelopoiesis), although always maintaining reduced numbers of lineage-specific cells.}

Previous reports showed an essential role of Notch in the onset of definitive hematopoiesis in the mouse P-Sp/AGM region, but with little or no effect on the primitive hematopoietic wave.²⁶, 35, 36, 37 In contrast, our gain-of-function approach has not only allowed us to determine that adult-type P-Sp/AGM hematopoiesis is impaired in Tie2-Cre;N1ICD embryos, but also revealed severe alterations in YS-derived primitive and transient definitive hematopoiesis. At E9.5, the YS of transgenic embryos contains above-normal numbers of c-Kit-low expressing cells, and low numbers of immature c-Kit^{cells that have a weak hematopoietic potential}in vitro. Constitutive Notch1 activation in Tie2^{cells moreover leads to the maintenance in the YS of CD31}^CD41^CD45^Mac1^TER119^{hemogenic endothelial cells and of c-Kit}^Tie2^{progenitors with}in vitro endothelial potential, bearing a c-Kit^/^Tie2^CD31^/^KDR^phenotype.

The in vivo mapping of Notch pathway activity in adult lymphoid tissues and in YS from E10.5 has being recently reported.⁵¹ No Hes1 expression on embryo HSC was found, despite Notch1/2 expression, suggesting that Notch signaling was not activated. MKs and erythrocytes expressed active Notch2, whereas much higher Notch1 levels were found in endothelial CD31^{precursors. The}in vitro transduction of constitutive active truncated Notch2 in CD34^{human cells showed increased numbers of basophilic immature erythroblast that did not reach mature stages.}⁵² A report using an N1ICD overexpression system in Tie2^{cells similar to ours also found, as we do, a block in erythroid cell development at the level of proerythroblasts, but quantification of the Ly6C}^{myeloid cells led the authors to conclude that myeloid differentiation was preserved.}⁵³ In our system, an extensive study of lineage markers and functional differentiation assays showed that N1ICD overexpression in Tie2^{cells produces a defect in the maturation process of the erythroid, megakaryocytic and myeloid lineages that prevents them from reaching the last maturation stages, resulting in an accumulation of erythroid and myeloid cells at different stages of differentiation, including erythro-myeloid progenitors. A similar defect in the}in vitro maturation of MKs has recently been described following activation of Dll4-Notch signaling in human cord blood CD34^cells.⁵⁴ In agreement with our results, N1ICD activation has been demonstrated to inhibit myeloid differentiation and to induce early stages of T-cell development from HSCs.⁵⁵

Our data additionally show that expression of N1ICD in Tie2^{cells prevents apoptosis and disrupts the proliferation and differentiation balance of embryonic hematopoietic cell lineages. Since the equilibrium between cellular proliferation and differentiation is strictly regulated,}⁵⁶ any alteration will affect lineage development. Our results suggest that Notch1 may normally function to fine-tune this process. Thus in transgenic YS, lineage-restricted progenitors (erythroblasts, megakaryoblasts and myeloblasts) would be unable to differentiate because of a continued proliferative stimulus elicited by constitutive N1ICD activity. This results in low numbers of HSCs and the accumulation of different intermediate progenitors, while impeding terminal differentiation of endothelial, erythroid, MK and myeloid cells. This phenotype would be compatible with a stem-cell exhaustion process that would occur early during primitive hematopoiesis.⁵⁶ In conclusion, our results show an unrevealed role of Notch signaling in YS-derived hematopoiesis by interfering in the proliferation and differentiation processes of lineage-restricted intermediate progenitors, producing a block of terminal differentiation.

Materials and Methods

Transgenic mice, embryo dissection and cell suspensions

Embryos overexpressing N1ICD-EGFP in Tie2-derived cells (Tie2-Cre;N1ICD) and their WT littermates were obtained by crossing Rosa26-N1ICD-EGFP³² (for simplicity N1ICD) with Tie2-Cre transgenic mice.³¹ Mice were maintained in the animal facility of the Centro Nacional de Investigaciones Cardiovasculares under pathogen-free conditions. All animal procedures conformed to EU Directive 2010/63EU and Recommendation 2007/526/EC regarding the protection of animals used for experimental and other scientific purposes, enforced in Spanish law under Real Decreto 1201/2005. Embryos were dissected and exsanguinated, and the embryonic blood and tissues (YS and the caudal portion of the embryo containing the P-Sp/AGM region) were recovered as described.⁵⁷ Cell suspensions were prepared from pooled embryonic tissues of the same gestational age by mechanical dissociation and cells were counted in a Neubauer chamber.

Flow cytometry analysis and cell sorting

The monoclonal antibodies (mAbs) used are detailed in Table 1. Cell suspensions were incubated with blocking solution (Fc block; BD Biosciences Inc., San José, CA, USA) for 10 min at 4°C. After washing, the cells were incubated with biotinylated, PE-, allophycocyanin-, PerCP-cyanin5.5 or PE-cyanin7-conjugated mAbs. Biotinylated mAbs were revealed by PE, allophycocyanin or PE-cyanin7-streptavidin. Cell debris and dead cells were discarded by 4′,6-diamidino-2-phenylindole (DAPI) exclusion after gating for forward scatter (FSC) versus side scatter (SSC). EGFP detection allowed the identification and purification of N1ICD-overexpressing EGFP^{cells. Specific mAb signals were defined against the background of irrelevant, isotype-matched mAbs. Experiments were performed in a FACSCalibur flow cytometer (BD Biosciences) and analyzed with the FlowJo-v6.3.4 software package (Tree Star Inc., Ashland, OR, USA). Stained cells were purified by FACS, using a FACSAria cell sorter (BD Biosciences) under sterile conditions. The purity of the recovered cells was above 98%.}

Table 1

Antibodies used

Antibody	Conjugation	Clone	Company
CD9	Biotin	KMC8	BD Biosciences
CD31 (PECAM-1)	PE/Biotin	MEC 13.3	BD Biosciences
CD41	PE	MWReg30	BD Biosciences
CD45	APC^a	30-F11	BD Biosciences
CD71	PE	{"type":"entrez-nucleotide","attrs":{"text":"R17217","term_id":"770827"}}R17217	e-Bioscience^b
c-Kit/CD117	APC	2B8	BD Biosciences
KDR/Flk1	PE	Avas 12α1	BD Biosciences
F4/80	APC	BM8	e-Bioscience
Mac1/CD11b	PE	M1/70	BD Biosciences
TER119	Biotin/PerCP-Cy5.5^c	TER-119	BioLegend^d
Tie2/TEK	Biotin	TEK4	e-Bioscience
Gr1	Biotin	RB6-8C5	BD Biosciences
Streptavidin	PE/APC/PE-Cy7^e		BioLegend
Anti-mouse vWF	Purified	Rabbit	Chemicon^f
Anti-mouse IgG₁	Purified	Rabbit	Chemicon
Anti-rabbit	Alexa-546	Goat	Invitrogen

^{APC: Allophycocyanin}

^{e-Bioscience, Hatfield, UK}

^{PerCP-Cy5.5: PerCP-Cyanin5.5}

^{BioLegend, San Diego, CA, USA}

^{Cy7: Cyanin7}

^{Chemicon International Inc., Temecula, CA, USA}

In situ hybridization, hematoxylin-eosin, May Grümwald-Giemsa and immunofluorescence staining

Embryos were dissected and fixed with 4% paraformaldehyde in phosphate-buffered saline overnight at 4 °C. Tissue embedding in paraffin, histological sectioning and hematoxylin and eosin staining were performed following standard protocols. In situ hybridization was carried out as described.⁵⁸ Cytospin preparations of cell suspensions (2.0–2.5 × 10^{) were dried at room temperature and fixed in 100% methanol, before being stained with May Grümwald-Giemsa solution (Materlab S.L., Madrid, Spain). vWF immunostainings were performed on slides of cell suspensions recovered from MethoCult cultures, as described.}⁵⁷

Confocal analysis

E9.5 WT and Tie2-Cre; N1ICD YSs were fixed 3 h in PFA 4%. Nuclei were stained with DAPI 1 : 1000 (124653; Merck, Readington, NJ, USA) for 15' before microscopy scanning. YSs were scanned for EGFP expression with a Leica SPE confocal microscope (Leica Microsystems, Solms, Germany) and LasAF software (Leica Microsystems).

Clonal assays for progenitor identification

Purified EGFP^{or WT YS cells (0.3–3.0 × 10}^/50μl) in DMEM (Lonza, Verviers, Belgium) were seeded in MethoCult M3434 methylcelullose medium (StemCell Technologies Inc., Vancouver, BC, Canada) and plated in triplicate in 48-well plates (Nunc, Thermo-Scientific, Roskilde, Denmark). Plates were kept at 37°C and 5% CO₂ for 7–10 days, and CFUs were counted by two independent observers under light microscopy (Leica-DFC 320 microscope, Leica Microsystems). Cell suspensions of the EGFP^{CFUs were transferred to fibronectin-coated plates (BD Biosciences) and supplemented with the pro-endothelial factors basic fibroblast growth factor and vascular endothelial growth factor (Peprotech, London, UK), or used for cytospin preparations. For MK progenitor quantification (MK-CFU), viable CD41}^{cells were plated in IMDM (Lonza) at 0.1–3.0 × 10}^{in MegaCult-C medium (StemCell Technologies Inc.), in 2-well chamber slides and incubated at 37°C for 7–10 days. Slides were dried before staining with acetylthiocholiniodide (Sigma-Aldrich, St. Louis, MO, USA) as indicated by the manufacturer.}

Proliferation, cell cycle and apoptosis analysis

Total YS cells were cultured over OP9 stromal cells pretreated with mytomycin (Sigma-Aldrich) in IMDM supplemented with IL7 (Peprotech), IL3 (Stem Cell), SCF (Peprotech), FLT3 ligand (Peprotech) and GM-CSF (Peprotech). Cells were seeded in 96-well plates (Nunc, Rochester, NY, USA) and cultured for 7 days. The cells were recovered, counted and analyzed by FACS to quantify EGFP^{cells. Aliquots of these cells were reseeded at 2 × 10}^{/well and recovered after 7 days. For cell-cycle analysis, FACS-purified EGFP}^{or WT cells (10}^{) were fixed in pre-cooled 70% ethanol, treated with RNAse, stained for DNA with PI, and analyzed in a FACSCalibur flow cytometer (BD Biosciences). In other experiments, 10}^{cells from WT or}Tie2-Cre;N1ICD YS were incubated with Hoechst 33342 (5 μg/ml; Invitrogen, Carlsbad, CA, USA) and verapamil (20 μM; Sigma-Aldrich) in IMDM at 37°C in the dark, and analyzed in a FACSVantage flow cytometer (BD Biosciences). Samples from adult thymus and a cell line were used as controls (data not shown). For in vivo S-phase cell analysis, pregnant females were intraperitoneally injected with 10 mM (100 μl) 5-ethinyl-2'-deoxyuridine (EdU; Invitrogen), and EGFP^{or WT cells were FACS purified. EdU incorporation was measured with the Click-iT EdU Flow Cytometry Kit (Invitrogen). Apoptotic cells were detected by flow cytometry using the 7-AAD Apoptosis Detection kit (Immunostep, Salamanca, Spain).}

Quantitative PCR

Total RNA from YS and FACS-purified cells were extracted with Trizol reagent (Invitrogen). cDNAs were synthesized using oligo-(dT) as primer and avian myeloblastosis virus reverse transcriptase (Promega, Madison, WI, USA). PCR amplifications were performed with the FastStart PCR amplification kit (Roche Diagnostics, Mannheim, Germany) in a PM-200 DNA Engine Cycler (Bio-Rad Laboratories Ltd, Hercules, CA, USA) using specific primer pairs for transcripts of interest (Supplementary Table 2). Quantification of GαS expression was performed as described by quantitative real-time PCR (qRT-PCR) in a Light-Cycler 2.0 system, using the Light-Cycler FastStart DNA Master SYBR green I kit (Roche Diagnostics).⁵⁹ The threshold cycle (C_T) of each reaction was calculated using the LightCycler 4.0 software (Roche, Basel, Switzerland) and the relative amount of specific GαS cDNA in each sample was determined. Specific RT-PCRs were performed with equivalent amounts of GαS cDNA. PCR products were electrophoretically separated on 2% agarose gels and quantified from nonsaturated PCRs (at 25, 30 or 35 cycles) by using GelDoc-XR and Quantity One software (Bio-Rad Laboratories Ltd). The densitometry values were referred to those obtained for the GαS transcript. The ratio of gene expression of each transcript between EGFPversus the mean of WT cells (ΔΔCt method) was calculated. To quantify primitive (βH1) and definitive (β1) hemoglobins RT-qPCR was performed on a CFX96 Real-Time System using the SsoFast Supermix EvaGreen (Bio-Rad) with specific primer pairs (Supplementary Table 2). The Bio-Rad CFX Manager software was used to calculate the C_T of each reaction.

Statistical analysis

Means and standard errors were calculated with GraphPad Prism 3.0 (GraphPad Software Inc., La Jolla, CA, USA) and P-values were obtained with the two-tailed Student's t-test.

Transgenic mice, embryo dissection and cell suspensions

Flow cytometry analysis and cell sorting

Table 1

Antibodies used

Antibody	Conjugation	Clone	Company
CD9	Biotin	KMC8	BD Biosciences
CD31 (PECAM-1)	PE/Biotin	MEC 13.3	BD Biosciences
CD41	PE	MWReg30	BD Biosciences
CD45	APC^a	30-F11	BD Biosciences
CD71	PE	{"type":"entrez-nucleotide","attrs":{"text":"R17217","term_id":"770827"}}R17217	e-Bioscience^b
c-Kit/CD117	APC	2B8	BD Biosciences
KDR/Flk1	PE	Avas 12α1	BD Biosciences
F4/80	APC	BM8	e-Bioscience
Mac1/CD11b	PE	M1/70	BD Biosciences
TER119	Biotin/PerCP-Cy5.5^c	TER-119	BioLegend^d
Tie2/TEK	Biotin	TEK4	e-Bioscience
Gr1	Biotin	RB6-8C5	BD Biosciences
Streptavidin	PE/APC/PE-Cy7^e		BioLegend
Anti-mouse vWF	Purified	Rabbit	Chemicon^f
Anti-mouse IgG₁	Purified	Rabbit	Chemicon
Anti-rabbit	Alexa-546	Goat	Invitrogen

^{APC: Allophycocyanin}

^{e-Bioscience, Hatfield, UK}

^{PerCP-Cy5.5: PerCP-Cyanin5.5}

^{BioLegend, San Diego, CA, USA}

^{Cy7: Cyanin7}

^{Chemicon International Inc., Temecula, CA, USA}

In situ hybridization, hematoxylin-eosin, May Grümwald-Giemsa and immunofluorescence staining

Confocal analysis

Clonal assays for progenitor identification

Proliferation, cell cycle and apoptosis analysis

Quantitative PCR

Statistical analysis

Means and standard errors were calculated with GraphPad Prism 3.0 (GraphPad Software Inc., La Jolla, CA, USA) and P-values were obtained with the two-tailed Student's t-test.

^{Immunology Department, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, km 2, 28220 Madrid, Spain}

^{Centro de Biología Molecular, Consejo Superior de Investigaciones Científicas (CBM-CSIC), Campus de Cantoblanco, 28049 Madrid, Spain}

^{Program of Cardiovascular Developmental Biology, Department of Cardiovascular Development and Repair, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain}

^{Cardiovascular Developmental Biology, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain. Tel: +34 91 4531334; Fax: +34 91 4531304; E-mail:}se.cinc@apmoplj

^{These authors contributed equally to this work.}

^{Current address: Laboratorio de Neurobiología Celular, Instituto de Investigación en Discapacidades Neurológicas, Universidad de Castilla-La Mancha, Almansa 14, 02006 Albacete, Spain.}

^Deceased.

Received 2013 Sep 20; Revised 2013 Dec 27; Accepted 2014 Jan 28.

Abstract

Loss-of-function studies have demonstrated the essential role of Notch in definitive embryonic mouse hematopoiesis. We report here the consequences of Notch gain-of-function in mouse embryo hematopoiesis, achieved by constitutive expression of Notch1 intracellular domain (N1ICD) in angiopoietin receptor tyrosine kinase receptor-2 (Tie2)-derived enhanced green fluorescence protein (EGFP^{) hematovascular progenitors. At E9.5, N1ICD expression led to the absence of the dorsal aorta hematopoietic clusters and of definitive hematopoiesis. The EGFP}^{transient multipotent progenitors, purified from E9.5 to 10.5}Tie2-Cre;N1ICD yolk sac (YS) cells, had strongly reduced hematopoietic potential, whereas they had increased numbers of hemogenic endothelial cells. Late erythroid cell differentiation stages and mature myeloid cells (Gr1^{, MPO}^{) were also strongly decreased. In contrast, EGFP}^{erythro-myeloid progenitors, immature and intermediate differentiation stages of YS erythroid and myeloid cell lineages, were expanded.}Tie2-Cre;N1ICD YS had reduced numbers of CD41^{megakaryocytes, and these produced reduced below-normal numbers of immature colonies}in vitro and their terminal differentiation was blocked. Cells from Tie2-Cre;N1ICD YS had a higher proliferation rate and lower apoptosis than wild-type (WT) YS cells. Quantitative gene expression analysis of FACS-purified EGFP^{YS progenitors revealed upregulation of Notch1-related genes and alterations in genes involved in hematopoietic differentiation. These results represent the first}in vivo evidence of a role for Notch signaling in YS transient definitive hematopoiesis. Our results show that constitutive Notch1 activation in Tie2^{cells hampers YS hematopoiesis of E9.5 embryos and demonstrate that Notch signaling regulates this process by balancing the proliferation and differentiation dynamics of lineage-restricted intermediate progenitors.}

Abstract

Notch is a highly conserved signaling pathway that regulates cell fate decisions in a variety of processes, including embryonic and adult hematopoiesis.¹, 2 Notch proteins and their ligands are transmembrane proteins and, upon ligand binding, the Notch intracellular domain (NICD) is released from the membrane by two consecutive proteolytic cleavage events and translocates to the nucleus. In the nucleus, NICD heterodimerizes with the transcriptional repressor CSL/RBPJK and converts it into a transcriptional activator. NICD/RBPJK target genes include those encoding basic helix-loop-helix transcription factors of the Hes and Hey families.¹ Truncated versions of Notch containing only the NICD result in constitutive activation of the pathway.³, 4, 5 Milner et al.⁶ demonstrated that Notch1 is expressed on human CD34^{bone marrow (BM) hematopoietic progenitor cells. Some years before, the human NOTCH1 gene was found to be disrupted by the chromosomal translocation t(7;9)(q34;q34.3), which yields truncated activated NOTCH1 (TAN-1) transcripts and results in T-cell acute lymphoblastic leukemia (T-ALL).}⁷ Indeed, more than 50% of T-ALL patients carry mutations leading to constitutive activation of NOTCH1 in T cell.⁸ It is now well known that NOTCH1 activation has critical roles at multiple stages of T-cell development.²

Notch signaling elements are expressed during embryonic and adult hematopoiesis.⁹, 10, 11 During embryogenesis, hematopoiesis takes place in two different sites, the yolk sac (YS) and the para-aortic splanchnopleura region (P-Sp).¹², 13, 14 Primitive hematopoiesis begins in the mouse at E7.0–7.5 in the YS blood islands as a transient wave that involves the production of primitive nucleated erythrocytes and certain myeloid progenitor cells.¹⁵ Adult-like definitive hematopoietic cells comprising erythrocytes, macrophages and granulocytes are produced in the YS at E8.25 from transient multipotent progenitors that lack long-term reconstitution potential.¹⁶ At E9.5–10, the intraembryonic P-Sp develops into the aorta-gonads-mesonephros (AGM) region and produces hematopoietic stem cells (HSCs) with long-term reconstitution potential that give rise to all cells of the adult hematopoietic system. These intraembryonic HSCs appear in a cell-autonomous manner as cell clusters in the floor of the dorsal aorta and the vitelline and umbilical arteries.¹⁷, 18, 19, 20 HSCs first generate committed, transit-amplifying progenitors that then differentiate into hematopoietic lineage-restricted progenitor cells.²¹ HSCs and progenitor cells migrate to colonize the fetal liver, the spleen, the thymus and finally the BM, which is the main hematopoietic organ during adult life.²¹

Normal hematopoiesis is the result of a highly regulated balance between self-renewal, proliferation and differentiation of stem and progenitor cells. Numerous reports based on in vitro and in vivo experiments strongly support a role for Notch in the self-renewal of hematopoietic stem and progenitor cells, and alterations to the Notch pathway disrupt hematopoietic differentiation.²², 23, 24, 25 Targeted inactivation of the Notch signaling components Notch1, RPBJk, Jag1 and Mib1 showed that Notch is essential for definitive hematopoiesis in the intraembryonic P-Sp/AGM region.²⁶, 27 The tyrosine kinase receptor-2 (Tie2) is expressed on vascular endothelium and on HSCs, and Tie2^{cells contain hemangioblasts able to differentiate into hematopoietic and endothelial lineages.}²⁸ Since both Tie2 and Notch1 intracellular domain (N1ICD) proteins have similar expression patterns very early in the YS blood island,²⁹, 30 we have used the Tie2-Cre driver line³¹ to overexpress Notch1 (N1ICD-EGFP (enhanced green fluorescence protein)).³² In this report, we show that constitutive Notch1 activation in Tie2^{cells impairs definitive hematopoiesis in the E9.5 embryo and produces severe alterations in YS transient definitive and primitive hematopoiesis. These results demonstrate that Notch signaling has an important function in YS-derived hematopoiesis by balancing the dynamics of proliferation and differentiation of lineage-restricted intermediate progenitors.}

Acknowledgments

We are grateful to B Palacios, P Martínez and C Ruiz for husbandry, Dr. M Alía and C Prado for their technical cytometry assistance and Dr. N Serrano and Dr. B de Andrés for helpful discussions. We thank S Bartlett (CNIC) for English editing. IC is supported by a fellowship from the Ministerio de Economía y Competitividad (MINECO). PMR has been supported by a PhD fellowship (FPU program, MINECO) and by the Fundación Leticia Castillejo. This work was funded by grants SAF2007-65265, SAF2009-12596, SAF2012-33916, and ISCIII08/0685 (MINECO) and SAL-0304-2006 (Madrid Regional Government) to MLG; SAF2007-62445 and SAF2010-17555, RD12/0019/0003 and RD12/0042/0005 (MINECO) to JLdlP. The CNIC is supported by the MINECO and the Pro-CNIC Foundation.

Acknowledgments

Glossary

7-AAD	7-aminoactinomycin D
AGM	aorta-gonads-mesonephros
BM	bone marrow
DAPI	4′,6-diamidino-2-phenylindole
EdU	5-ethinyl-2′-deoxyuridine
EGFP	enhanced green fluorescence protein
FSC	forward scatter
HSC	hematopoietic stem cells
MFI	mean fluorescence intensity
MK	megakaryocyte
mAbs	monoclonal antibodies
N1ICD	Notch1 intracellular domain
P-Sp	para-aortic splanchnopleura region
PI	propidium iodide
qRT-PCR	quantitative real-time PCR
SSC	side scatter
Tie2	tyrosine kinase receptor-2
vWF	von Willebrand factor
WT	wild type
YS	yolk sac

Glossary

Notes

The authors declare no conflict of interest.

Notes

Footnotes

Supplementary Information accompanies this paper on Cell Death and Differentiation website (http://www.nature.com/cdd)

Author contributions

MARM, MLG and JLP designed research; IC, PMR, LL and MSA performed research; IC and MLG contributed analytical tools; IC, PMR, MARM, MLG and JLP analyzed and interpreted data; IC and MLG performed statistical analysis; JLP, MLG, IC and PMR wrote the manuscript.

Edited by R De Maria

Footnotes

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Notch1 regulates progenitor cell proliferation and differentiation during mouse yolk sac hematopoiesis

Results

Constitutive N1ICD expression in Tie2 progenitors impairs definitive intraembryonic hematopoiesis

N1ICD expression reduces the hematopoietic potential and favors the endothelial phenotype of Tie2 cells in the YS

Erythroid and megakaryocyte development is blocked in Tie2-Cre;N1ICD mice

Phenotypic characterization of myeloid immature progenitor cell populations in WT and Tie2-Cre;N1ICD YS at E9.5

Notch1 activation on Tie2-derived YS cells dysregulates the transcriptional program that initiates hematopoietic differentiation

N1ICD expression in Tie2 cells induces high proliferation, cell-cycle activation and reduced apoptosis

Constitutive N1ICD expression in Tie2 progenitors impairs definitive intraembryonic hematopoiesis

N1ICD expression reduces the hematopoietic potential and favors the endothelial phenotype of Tie2 cells in the YS

Erythroid and megakaryocyte development is blocked in Tie2-Cre;N1ICD mice

Phenotypic characterization of myeloid immature progenitor cell populations in WT and Tie2-Cre;N1ICD YS at E9.5

Notch1 activation on Tie2-derived YS cells dysregulates the transcriptional program that initiates hematopoietic differentiation

N1ICD expression in Tie2 cells induces high proliferation, cell-cycle activation and reduced apoptosis

Discussion

Materials and Methods

Transgenic mice, embryo dissection and cell suspensions

Flow cytometry analysis and cell sorting

Table 1

In situ hybridization, hematoxylin-eosin, May Grümwald-Giemsa and immunofluorescence staining

Confocal analysis

Clonal assays for progenitor identification

Proliferation, cell cycle and apoptosis analysis

Quantitative PCR

Statistical analysis

Transgenic mice, embryo dissection and cell suspensions

Flow cytometry analysis and cell sorting

Table 1

In situ hybridization, hematoxylin-eosin, May Grümwald-Giemsa and immunofluorescence staining

Confocal analysis

Clonal assays for progenitor identification

Proliferation, cell cycle and apoptosis analysis

Quantitative PCR

Statistical analysis

Abstract

Acknowledgments

Glossary

Notes

Footnotes

Constitutive N1ICD expression in Tie2^{progenitors impairs definitive intraembryonic hematopoiesis}

N1ICD expression reduces the hematopoietic potential and favors the endothelial phenotype of Tie2^{cells in the YS}

N1ICD expression in Tie2^{cells induces high proliferation, cell-cycle activation and reduced apoptosis}

Constitutive N1ICD expression in Tie2^{progenitors impairs definitive intraembryonic hematopoiesis}

N1ICD expression reduces the hematopoietic potential and favors the endothelial phenotype of Tie2^{cells in the YS}

N1ICD expression in Tie2^{cells induces high proliferation, cell-cycle activation and reduced apoptosis}