Niche of harmful alga Aureococcus anophagefferens revealed through ecogenomics.
Journal: 2011/May - Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Abstract:
Harmful algal blooms (HABs) cause significant economic and ecological damage worldwide. Despite considerable efforts, a comprehensive understanding of the factors that promote these blooms has been lacking, because the biochemical pathways that facilitate their dominance relative to other phytoplankton within specific environments have not been identified. Here, biogeochemical measurements showed that the harmful alga Aureococcus anophagefferens outcompeted co-occurring phytoplankton in estuaries with elevated levels of dissolved organic matter and turbidity and low levels of dissolved inorganic nitrogen. We subsequently sequenced the genome of A. anophagefferens and compared its gene complement with those of six competing phytoplankton species identified through metaproteomics. Using an ecogenomic approach, we specifically focused on gene sets that may facilitate dominance within the environmental conditions present during blooms. A. anophagefferens possesses a larger genome (56 Mbp) and has more genes involved in light harvesting, organic carbon and nitrogen use, and encoding selenium- and metal-requiring enzymes than competing phytoplankton. Genes for the synthesis of microbial deterrents likely permit the proliferation of this species, with reduced mortality losses during blooms. Collectively, these findings suggest that anthropogenic activities resulting in elevated levels of turbidity, organic matter, and metals have opened a niche within coastal ecosystems that ideally suits the unique genetic capacity of A. anophagefferens and thus, has facilitated the proliferation of this and potentially other HABs.
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Proc Natl Acad Sci U S A 108(11): 4352-4357

Niche of harmful alga <em>Aureococcus anophagefferens</em> revealed through ecogenomics

+24 authors

Light Harvesting.

Phytoplankton rely on light to photosynthetically fix carbon dioxide into organic carbon, but the turbid, low-light environment characteristic of estuaries and intense shading during dense algal blooms (Fig. 1 B and C) can strongly limit photosynthesis. A. anophagefferens is better adapted to low light than the comparative phytoplankton species, which requires at least threefold higher light levels to achieve maximal growth rates (Fig. 2A). Its genome contains the full suite of genes involved in photosynthesis, including 62 genes encoding light-harvesting complex (LHC) proteins (Fig. 2A). This is 1.5–3 times more than other eukaryotic phytoplankton sequenced thus far (Fig. 2A and SI Appendix, Table S7) and a feature that likely enhances adaptation to low and/or dynamic light conditions found in turbid estuaries. LHC proteins bind antenna chlorophyll and carotenoid pigments that augment the light-capturing capacity of the photosynthetic reaction centers (18, 19). Twenty-six A. anophagefferens LHC genes belong to a group that has only six representatives in T. pseudonana and one representative in P. tricornutum (branch PHYMKG in Fig. 3 and SI Appendix, Fig. S1) but are similar to the multicellular brown macroalgae, Ectocarpus siliculosus (20). Similar LHC genes in the microalgae Emiliania huxleyi have recently been shown to be up-regulated under low light (21). We hypothesize that these LHC genes encode the major light-harvesting proteins for A. anophagefferens and that the enrichment of these proteins imparts a competitive advantage in acquiring light under the low-irradiance conditions that prevail during blooms (Fig. 1C).

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Comparisons of gene compliment between A. anophagefferens and other co-occurring phytoplankton species. Aa, A. anophagefferens; Pt, P. tricornutum; Tp, T. pseudonana; Ot, O. tauri; Ol, O. lucimarinus; S1, Synechococcus clone CC9311; S2, Synechococcus clone CC9902. (A) The number of light-harvesting complex (LHC) genes present in each phytoplankton genome (red bars; left axis) and Imax, the irradiance level required to achieve maximal growth rates in each phytoplankton (black squares; right axis) are shown. Among these species, A. anophagefferens possesses the greatest number of LHC genes, achieves a maximal growth rate at the lowest level of light, and blooms when light levels are low. (B) The number of genes associated with the degradation of nitriles, asparagine, and urea in each phytoplankton genome. A. anophagefferens grows efficiently on organic nitrogen and possesses more nitrilase, asparaginase, and urease genes than other phytoplankton. (C) Interspecies comparison of the genes encoding proteins that contain the metals Se, Cu, Mo, Ni, and Co (left axis) and Semax, the selenium level (added as selenite shown as log concentrations) required to achieve maximal growth rates in A. anophagefferens, P. tricornutum, T. pseudonana, and Synechococcus (white circles; right axis). The range of dissolved selenium concentrations found in estuaries is depicted as a yellow bar on the right y axis. A. anophagefferens has the largest number of proteins containing Se, Cu, Mo, and Ni and blooms exclusively in shallow estuaries where inventories of these metals are high. SI Appendix contains details of irradiance- and Se-dependent growth data and Se concentrations in estuaries.

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Phylogenetic tree constructed from amino acid sequences of predicted LHC proteins from two diatoms (P. tricornutum and T. pseudonana; black branches), two Ostreococcus species (O. tauri and O. lucimarinus; green branches), and A. anophagefferens (red branches). The tree constructed in MEGA4 (SI Appendix, Fig. S1) is displayed here after manipulation of the original branch lengths in Hypertree (http://kinase.com/tools/HyperTree.html) to aid visualization of major features of the tree. None of the Aureococcus LHCs were closely related to green plastid lineage LHCs, although four belonged to a group found in both the green and red plastid lineages (group I). None of the Aureococcus LHCs clustered with the major fucoxanthin-chlorophyll binding proteins (FCP) of diatoms and other heterokonts (major FCP group). However, many Aureococcus LHCs did group with similar sequences from P. tricornutum and T. pseudonana (as well as LHCs from other red-lineage algae not included in this tree; groups A–K). There were also five groups of A. anophagefferens LHCs that were not closely related to any other LHCs (Aur1 to Aur5). Group G includes 16 LHCs from A. anophagefferens and 2 LHCs from T. pseudonana, and it shares a unique PHYMKG motif near the end of helix two, with 10 additional A. anophagefferens LHCs plus 5 more from the diatoms. Cyanobacteria such as Synechococcus do not possess LHC proteins.

Organic Matter Use.

In addition to being well-adapted to low light, A. anophagefferens also outcompetes other phytoplankton in estuaries with elevated organic matter concentrations (6) (Fig. 1C), and can survive extended periods with no light (22). Consistent with these observations, the genome of A. anophagefferens contains a large number of genes that may permit the degradation of organic compounds to support heterotrophic metabolism. For example, its genome encodes proteins involved in the transport of oligosaccharides and sugars that are not found in competing phytoplankton, including genes for glycerol, glucose, and d-xylose uptake (SI Appendix, Table S8). The A. anophagefferens genome also encodes more nucleoside sugar transporters and major facilitator family sugar transporters than other comparative phytoplankton species (SI Appendix, Table S8). It is highly enriched in genes associated with the degradation of mono-, di-, oligo-, and polysaccharides as well as sulfonated polysaccharides. A. anophagefferens possesses 47 sufatase genes, including those targeting sulfonated polysaccharides such as glucosamine-(N-acetyl)-6-sulfatases, whereas the diatoms contain a total of three to four sulfatases and the comparative picoplankton contain none (SI Appendix, Table S9). A. anophagefferens also possesses many more genes involved in carbohydrate degradation than competing phytoplankton (85 vs. 4–29 genes in comparative phytoplankton), including 29 such genes present only in A. anophagefferens (Fig. 4 and SI Appendix, Tables S10 and S11). Collectively, these genes (SI Appendix, Tables S9, S10, S11, and S12) provide this alga with unique metabolic capabilities regarding the degradation of an array of organic carbon compounds, many of which may not be accessible to other phytoplankton. In an ecosystem setting, such a supplement of organic carbon would be critical for population proliferation within the low-light environments present in estuaries, particularly during dense algal blooms (Fig. 1C).

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Genes encoding for enzymes involved in degrading organic carbon compounds in A. anophagefferens. The graph displays the portion and names of the genes encoding for functions that are unique to A. anophagefferens (red; 53%), enriched in A. anophagefferens relative to the six comparative phytoplankton (34%; green), and present at equal or lower numbers in A. anophagefferens relative to the six comparative phytoplankton (13%; blue). The number of genes present in multiple copies in A. anophagefferens is shown in parentheses. Further details regarding these genes are presented in SI Appendix, Tables S10 and S11.

A. anophagefferens, like many HABs, blooms when inorganic nitrogen levels are low but organic nitrogen levels are elevated (Fig. 1C) (13), and A. anophagefferens is known to efficiently metabolize organic compounds for nitrogenous nutrition (6, 23). Notably, this niche strategy is reflected within the A. anophagefferens genome, which encodes transporters specific for a diverse set of organic nitrogen compounds including urea, amino acids, purines, nucleotide sugars, nucleosides, peptides, and oligopeptides (SI Appendix, Table S8) (24). Relative to competing phytoplankton, A. anophagefferens is enriched in genes encoding enzymes that degrade organic nitrogen compounds, such as nitriles, asparagine, and urea (Fig. 2B). A. anophagefferens is also the only species among the phytoplankton genomes examined that possesses a membrane-bound dipeptidase, several histidine ammonia lyases, tripeptidyl peptidase, and several other enzymes (SI Appendix, Table S13) that could collectively play a role in metabolizing organic nitrogen compounds that are not bioavailable to other phytoplankton. Furthermore, the A. anophagefferens genome also contains enzymes that degrade amino acids, peptides, proteins, amides, amides, and nucleotides, often possessing more copies of these genes than competing phytoplankton (SI Appendix, Table S13). This characteristic, along with its unique gene set, may provide A. anophagefferens with a greater capacity to use organic compounds for nitrogenous nutrition compared with its competitors, a hypothesis supported by its dominance in systems with elevated ratios of dissolved organic nitrogen to dissolved inorganic nitrogen and the reduction in dissolved organic nitrogen concentrations often observed during the initiation of brown tides (6, 25).

Metalloenzymes.

A. anophagefferens blooms in shallow, enclosed estuaries (6) where the concentrations of metals and elements like selenium are elevated (2628), but it never dominates deep estuaries or continental shelf regions (6) that are characterized by lower metal and trace element inventories (2628). A. anophagefferens has a large and absolute requirement for some trace elements, such as selenium (Fig. 2C). In comparison, phytoplankton, such as Synechococcus, do not require this element, whereas others, such as T. pseudonana and P. tricornutum, have lower selenium requirements for maximal growth (Fig. 2C). The A. anophagefferens genome is consistent with these observations, being enriched in numerous classes of proteins that require metals and elements like selenium as cofactors (Fig. 2C). It possesses at least 56 genes encoding selenocysteine-containing proteins, two times the number present in the O. lucimarinus genome, which previously had the largest known eukaryotic selenoproteome (11, 29), and fourfold more than the diatom genomes (Fig. 2C). The A. anophagefferens selenoproteome includes nearly all known eukaryotic selenoproteins as well as selenoproteins that were previously described only in bacteria (29) and several selenoproteins that have never been described in any other organism (SI Appendix, Table S14). In addition, several selenoprotein families are represented by multiple isozymes (SI Appendix, Table S14). One-half of the selenoproteins are methionine sulfoxide reductases, thioredoxin reductases, glutathione peroxidases, glutaredoxins, and peroxiredoxins (SI Appendix, Table S14). Together, these enzymes help protect cells against oxidative stress in the dynamic and ephemeral conditions present in estuaries through the removal of hydroperoxides and the repair of oxidatively damaged proteins. Moreover, selenocysteine residues are often superior catalytic groups compared with cysteine (3032), and thus, they allow A. anophagefferens to more efficiently execute multiple metabolic processes and increase its competitiveness relative to other phytoplankton in the anthropogenically modified estuaries where it blooms.

The A. anophagefferens genome is also enriched in genes encoding for molybdenum-, copper-, and nickel-containing enzymes (Fig. 2C). For example, the A. anophagefferens genome includes two times the number of genes encoding molybdenum-containing oxidases found in competing species (6 vs. 1–3 genes) (Fig. 2C and SI Appendix, Tables S15 and S16) and has the largest number of molybdenum-specific transporters (SI Appendix, Table S8). Similarly, A. anophagefferens possesses four times more genes that encode copper-containing proteins than its competitors (27 vs. 1–6 genes) (Fig. 2C), including 5 multicopper oxidases and 20 tyrosinase-like proteins (SI Appendix, Tables S15 and S16). Several of the A. anophagefferens tyrosinase and multicopper oxidase family proteins are heavily glycosylated (more than four glycosylation sites) (SI Appendix, Table S16) and thus, are likely secretory proteins, whereas the few present in the other comparative algal species are not. These copper-containing enzymes degrade lignin, catalyze the oxidation of phenolics, and can have antimicrobial properties (33, 34) and thus, may provide nutrition or confer protection to A. anophagefferens cells. A. anophagefferens is also the only phytoplankton species with a homolog of the CutC copper homeostasis protein, which permits efficient cellular trafficking of this metal (SI Appendix, Table S8). With three nickel-requiring ureases, A. anophagefferens has more nickel-containing enzymes than other comparative phytoplankton (Fig. 2 B and C). Consistent with its ecogenomic profile, these ureases allow A. anophagefferens to meet its daily N demand from urea, whereas other phytoplankton do not (35). Perhaps to support the synthesis and use of ureases, A. anophagefferens is the only comparative phytoplankton species with a high-affinity nickel transporter (HoxN) (36). A. anophagefferens is not universally enriched in metalloenzymes, because other phytoplankton contain equal numbers of cobalt-containing enzymes (Fig. 2C). However, the formation of blooms exclusively in shallow estuaries ensures that A. anophagefferens has access to a rich supply of the selenium, copper, and nickel required to synthesize these ecologically important and catalytically superior enzymes (30, 31, 37).

Microbial Defense.

Although genes associated with the adaptation to low light, the use of organic matter, and metals permit A. anophagefferens to dominate a specific geochemical niche found within estuaries, genes involved in the production of compounds that inhibit predators and competitors may further promote blooms (2). Although specific toxins have yet to be identified in A. anophagefferens, it is grazed at a low rate during blooms (2, 6), and its genome contains two to seven times more genes involved in the synthesis of secondary metabolites than the comparative phytoplankton genomes (SI Appendix, Fig. S2). A. anophagefferens also possesses a series of genes involved in the synthesis of putative antimicrobial compounds that are largely absent from the competing phytoplankton species (SI Appendix, Table S17). For example, A. anophagefferens has five berberine bridge enzymes involved in the synthesis of toxic isoquinoline alkaloids (38, 39) (SI Appendix, Table S17). A. anophagefferens uniquely possesses a membrane attack complex gene and multiple phenazine biosynthetase genes (SI Appendix, Table S17) that encode enzymes that may provide defense against microbes and/or protistan grazers (40, 41). There are two- to fourfold more ATP-binding cassette (ABC) transporters in A. anophagefferens compared with competing species (112 vs. 30–54 ABC transporters) (SI Appendix, Table S8), and it is specifically enriched in ABC multidrug efflux pumps that protect cells from toxic xenobiotics and endogenous metabolites (42, 43). Finally, the A. anophagefferens genome encodes 16-fold more Sel-1 genes (130 vs. 0–8 genes) (Table S6), 4-fold more ion channels (82 vs. 1–19 ion channels) (SI Appendix, Table S8), 4-fold more protein kinases, and 2-fold more WD40 domain genes than other phytoplankton (SI Appendix, Table S6). These genes may collectively mediate elaborate cell signaling and sensing by dense bloom populations (4446), processes that would be important for detecting competitors, predators, other A. anophagefferens cells, and the environment. Together, genes involved in the synthesis of microbial deterrents, export of toxic compounds, and cell signaling may contribute to the proliferation of this species with reduced population losses and thus, assist in promoting these HABs (2).

Conclusions.

The global expansion of human populations along coastlines has led to a progressive enrichment in turbidity (47), organic matter, including organic nitrogen (1, 47, 48), and metals (26, 28) in estuaries. Matching the expansion of HAB events around the world in recent decades, A. anophagefferens blooms were an unknown phenomenon before 1985 but have since become chronic, annual events in US and South African estuaries (6), with the potential for further expansion. The unique gene complement of A. anophagefferens encodes a disproportionately greater number of proteins involved in light harvesting and organic matter use as well as metal and selenium-requiring enzymes relative to competing phytoplankton. Collectively, these genes reveal a niche characterized by conditions (low light, high organic matter, and elevated metal levels) that have become increasingly prevalent in anthropogenically modified estuaries, suggesting that human activities have enabled the proliferation of these HABs. In estuaries that host A. anophagefferens blooms, anthropogenic nutrient loading promotes algal growth and as a result, elevated levels of organic matter and turbidity (6), whereas high concentrations of metals have been attributed to maritime paints and some fertilizers (27, 49). Collectively, these findings establish a context within which to prevent and control HABs, specifically by ameliorating anthropogenically altered aspects of marine environments that harmful phytoplankton are genomically predisposed to exploit. Like A. anophagefferens, many HAB-forming dinoflagellates are known to exploit organic forms of carbon and nitrogen for growth (14), grow well under low light (50), and have elevated requirements of copper, molybdenum, and selenium (51, 52). Continued ecogenomic analyses of HABs will reveal the extent to which these events can be attributed to human activities that have transformed coastal ecosystems to suit the genetic capacity of these algae.

Supplementary Material

Supporting Information:
School of Marine and Atmospheric Sciences, Stony Brook University, Southampton, NY 11968;
School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794-5000;
Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543;
Department of Microbiology, University of Tennessee, Knoxville, TN 37996;
US Department of Energy Joint Genome Institute, Walnut Creek, CA 94598;
Division of Genetics, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115;
Department of Earth and Environmental Sciences, Rutgers University, Newark, NJ 07102;
Chemical Sciences and
Biosciences Divisions, Oak Ridge National Laboratory, Oak Ridge, TN 37830;
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney 2109, New South Wales, Australia;
College of Earth, Ocean, and Environment, University of Delaware, Lewes, DE 19958;
Department of Environmental Earth System Science, Stanford University, Stanford, CA 94305;
Massachusetts, Institute of Technology and Woods Hole Oceanographic Institution Joint Program in Chemical Oceanography, Woods Hole, MA 02543; and
Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA 02543
To whom correspondence may be addressed. E-mail: ude.koorbynots@relbog.rehpotsirhc or vog.lbl@veirogirGVI.
Edited by David M. Karl, University of Hawaii, Honolulu, HI, and approved January 26, 2011 (received for review October 29, 2010)

Author contributions: C.J.G. and I.V.G. designed research; C.J.G., D.L.B., S.T,D., S.W.W., J.L.C., L.L.W., B.D.D., M.S., N.C.V., A.K., A.T., J.P., E.A.L., S.L., S.C.T., E.A.W., F.K., M.A.M., and I.V.G. performed research; I.T.P., G.M.B., and I.V.G. contributed new reagents/analytic tools; C.J.G., D.L.B., S.T.D., S.W.W., A.S., A.V.L., Y.Z., J.L.C., L.L.W., A.B.K., B.D.D., M.S., N.C.V., A.K., A.T., T.K.H.-L., A.M.B., Y.-Z.T., G.R.L., K.J.C., E.M.B., V.N.G., and I.V.G. analyzed data; and C.J.G., S.T.D., S.W.W., J.L.C., A.B.K., M.A.S., V.N.G., and I.V.G. wrote the paper.

D.B., S.D., and S.W. contributed equally to this work.
Edited by David M. Karl, University of Hawaii, Honolulu, HI, and approved January 26, 2011 (received for review October 29, 2010)
Freely available online through the PNAS open access option.

Abstract

Harmful algal blooms (HABs) cause significant economic and ecological damage worldwide. Despite considerable efforts, a comprehensive understanding of the factors that promote these blooms has been lacking, because the biochemical pathways that facilitate their dominance relative to other phytoplankton within specific environments have not been identified. Here, biogeochemical measurements showed that the harmful alga Aureococcus anophagefferens outcompeted co-occurring phytoplankton in estuaries with elevated levels of dissolved organic matter and turbidity and low levels of dissolved inorganic nitrogen. We subsequently sequenced the genome of A. anophagefferens and compared its gene complement with those of six competing phytoplankton species identified through metaproteomics. Using an ecogenomic approach, we specifically focused on gene sets that may facilitate dominance within the environmental conditions present during blooms. A. anophagefferens possesses a larger genome (56 Mbp) and has more genes involved in light harvesting, organic carbon and nitrogen use, and encoding selenium- and metal-requiring enzymes than competing phytoplankton. Genes for the synthesis of microbial deterrents likely permit the proliferation of this species, with reduced mortality losses during blooms. Collectively, these findings suggest that anthropogenic activities resulting in elevated levels of turbidity, organic matter, and metals have opened a niche within coastal ecosystems that ideally suits the unique genetic capacity of A. anophagefferens and thus, has facilitated the proliferation of this and potentially other HABs.

Keywords: genomics, proteome, comparative genomics, eutrophication
Abstract

Harmful algal blooms (HABs) are caused by phytoplankton that have a negative impact on ecosystems and coastal fisheries worldwide (14) and cost the US economy alone hundreds of millions of dollars annually (5). The frequency and impacts of HABs have intensified in recent decades, and anthropogenic processes, including eutrophication, have been implicated in this expansion (13). Although there is great interest in mitigating the occurrence of HABs, traditional approaches that have characterized biogeochemical conditions present during blooms do not identify the aspects of the environment that are favorable to an individual algal species. Predicting where, when, and under what environmental conditions HABs will occur has further been inhibited by a limited understanding of the cellular attributes that facilitate the proliferation of one phytoplankton species to the exclusion of others.

Aureococcus anophagefferens is a pelagophyte that causes harmful brown tide blooms with densities exceeding 10 cells mL for extended periods in estuaries in the eastern United States and South Africa (6). Brown tides do not produce toxins that poison humans but have decimated multiple fisheries and seagrass beds because of toxicity to bivalves and extreme light attenuation, respectively (6). Brown tides are a prime example of the global expansion of HABs, because these blooms had never been documented before 1985 but have recurred in the United States and South Africa annually since that time (6). Like many other HABs, A. anophagefferens blooms in shallow, anthropogenically modified estuaries when levels of light and inorganic nutrients are low and organic carbon and nitrogen concentrations are elevated (13).

For this study, we used an ecogenomic approach to assess the extent to which the gene set of A. anophagefferens may permit its dominance under the environmental conditions present in estuaries during brown tides. We characterized the biogeochemical conditions present in estuaries before, during, and after A. anophagefferens blooms. Sequencing this HAB genome (A. anophagefferens), we compared its gene content to those of six phytoplankton species identified through metaproteomics to co-occur with this alga during blooms events. Using this ecogenomic approach, we investigated how the gene sets of A. anophagefferens differ from the six comparative phytoplankton species and how these differences may affect the ability of A. anophagefferens to compete in the physical (e.g., light harvesting), chemical (e.g., nutrients, organic matter, and trace metals), and ecological (e.g., defense against predators and allelopathy) environment present during brown tides.

Genes with known functions were identified using Swiss-Prot, a curated protein sequence database, with an e-value cutoff of <10 (13). Pfam domains are sequences identified from a database of protein families represented by multiple sequence alignments and hidden Markov models (14). The compressed nature of P. tricornutum cells (11 × 2.5 μm) makes its biovolume smaller than T. pseudonana.

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Acknowledgments

Assembly and annotations of A. anophagefferens are available from JGI Genome Portal at http://www.jgi.doe.gov/Aureococcus. Genome sequencing, annotation, and analysis were conducted by the US Department of Energy Joint Genome Institute supported by the Office of Science of the US Department of Energy under Contract No. DE-AC02-05CH11231. Efforts were also supported by National Oceanic and Atmospheric Administration Sea Grant Awards NA07OAR4170010 and NA10OAR4170064 to Stony Brook University via New York Sea Grant, National Oceanic and Atmospheric Administration Center for Sponsored Coastal Ocean Research Award NA09NOS4780206 to Woods Hole Oceanographic Institution, National Institutes of Health Grant GM061603 to Harvard University, and National Science Foundation Award IOS-0841918 to University of Tennessee.

Acknowledgments

Footnotes

The authors declare no conflict of interest.

This article is a PNAS Direct Submission.

Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. {"type":"entrez-nucleotide","attrs":{"text":"ACJI00000000","term_id":"320114799","term_text":"ACJI00000000"}}ACJI00000000).

This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1016106108/-/DCSupplemental.

Footnotes

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