Proc Natl Acad Sci U S A 84(10): 3365-3369

Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.

Abstract

A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated dishes. COS cells expressing a CD2 cDNA isolated by this method readily formed rosettes with sheep as well as human and other mammalian erythrocytes. Pretreatment of transfected COS cells with anti-CD2 antibody, or pretreatment of human erythrocytes with anti-LFA-3 antibody, abolished rosette formation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Image
on p.3367

Image
on p.3368

Image
on p.3369

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Bach JF, Dormont J, Dardenne M, Balner H. In vitro rosette inhibition by antihuman antilymphocyte serum. Correlation with skin graft prolongation in subhuman primates. Transplantation. 1969 Sep;8(3):265–280. [PubMed] [Google Scholar]
Brain P, Gordon J, Willetts WA. Rosette formation by peripheral lymphocytes. Clin Exp Immunol. 1970 May;6(5):681–688.[PMC free article] [PubMed] [Google Scholar]
Coombs RR, Gurner BW, Wilson AB, Holm G, Lindgren B. Rosette-formation between human lymphocytes and sheep red cells not involving immunoglobulin receptors. Int Arch Allergy Appl Immunol. 1970;39(5-6):658–663. [PubMed] [Google Scholar]
Howard FD, Ledbetter JA, Wong J, Bieber CP, Stinson EB, Herzenberg LA. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation. J Immunol. 1981 Jun;126(6):2117–2122. [PubMed] [Google Scholar]
Kamoun M, Martin PJ, Hansen JA, Brown MA, Siadak AW, Nowinski RC. Identification of a human T lymphocyte surface protein associated with the E-rosette receptor. J Exp Med. 1981 Jan 1;153(1):207–212.[PMC free article] [PubMed] [Google Scholar]
Verbi W, Greaves MF, Schneider C, Koubek K, Janossy G, Stein H, Kung P, Goldstein G. Monoclonal antibodies OKT 11 and OKT 11A have pan-T reactivity and block sheep erythrocyte "receptors". Eur J Immunol. 1982 Jan;12(1):81–86. [PubMed] [Google Scholar]
Bernard A, Gelin C, Raynal B, Pham D, Gosse C, Boumsell L. Phenomenon of human T cells rosetting with sheep erythrocytes analyzed with monoclonal antibodies. "Modulation" of a partially hidden epitope determining the conditions of interaction between T cells and erythrocytes. J Exp Med. 1982 May 1;155(5):1317–1333.[PMC free article] [PubMed] [Google Scholar]
Palacios R, Martinez-Maza O. Is the E receptor on human T lymphocytes a "negative signal receptor"? J Immunol. 1982 Dec;129(6):2479–2485. [PubMed] [Google Scholar]
Martin PJ, Longton G, Ledbetter JA, Newman W, Braun MP, Beatty PG, Hansen JA. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J Immunol. 1983 Jul;131(1):180–185. [PubMed] [Google Scholar]
Reed JC, Tadmori W, Kamoun M, Koretzky G, Nowell PC. Suppression of interleukin 2 receptor acquisition by monoclonal antibodies recognizing the 50 KD protein associated with the sheep erythrocyte receptor on human T lymphocytes. J Immunol. 1985 Mar;134(3):1631–1639. [PubMed] [Google Scholar]
Krensky AM, Sanchez-Madrid F, Robbins E, Nagy JA, Springer TA, Burakoff SJ. The functional significance, distribution, and structure of LFA-1, LFA-2, and LFA-3: cell surface antigens associated with CTL-target interactions. J Immunol. 1983 Aug;131(2):611–616. [PubMed] [Google Scholar]
Meuer SC, Hussey RE, Fabbi M, Fox D, Acuto O, Fitzgerald KA, Hodgdon JC, Protentis JP, Schlossman SF, Reinherz EL. An alternative pathway of T-cell activation: a functional role for the 50 kd T11 sheep erythrocyte receptor protein. Cell. 1984 Apr;36(4):897–906. [PubMed] [Google Scholar]
O'Flynn K, Russul-Saib M, Ando I, Wallace DL, Beverley PC, Boylston AW, Linch DC. Different pathways of human T-cell activation revealed by PHA-P and PHA-M. Immunology. 1986 Jan;57(1):55–60.[PMC free article] [PubMed] [Google Scholar]
Yang SY, Chouaib S, Dupont B. A common pathway for T lymphocyte activation involving both the CD3-Ti complex and CD2 sheep erythrocyte receptor determinants. J Immunol. 1986 Aug 15;137(4):1097–1100. [PubMed] [Google Scholar]
O'Flynn K, Krensky AM, Beverley PC, Burakoff SJ, Linch DC. Phytohaemagglutinin activation of T cells through the sheep red blood cell receptor. Nature. 1985 Feb 21;313(6004):686–687. [PubMed] [Google Scholar]
O'Flynn K, Knott LJ, Russul-Saib M, Abdul-Gaffar R, Morgan G, Beverley PC, Linch DC. CD2 and CD3 antigens mobilize Ca2+ independently. Eur J Immunol. 1986 May;16(5):580–584. [PubMed] [Google Scholar]
Little PF, Treisman R, Bierut L, Seed B, Maniatis T. Plasmid vectors for the rapid isolation and transcriptional analysis of human beta-globin gene alleles. Mol Biol Med. 1983 Dec;1(5):473–488. [PubMed] [Google Scholar]
Gubler U, Hoffman BJ. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. [PubMed] [Google Scholar]
Wysocki LJ, Sato VL. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848.[PMC free article] [PubMed] [Google Scholar]
Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. 1981 Jan;23(1):175–182. [PubMed] [Google Scholar]
Sandri-Goldin RM, Goldin AL, Levine M, Glorioso JC. High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion. Mol Cell Biol. 1981 Aug;1(8):743–752.[PMC free article] [PubMed] [Google Scholar]
Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. [PubMed] [Google Scholar]
Sussman DJ, Milman G. Short-term, high-efficiency expression of transfected DNA. Mol Cell Biol. 1984 Aug;4(8):1641–1643.[PMC free article] [PubMed] [Google Scholar]
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed] [Google Scholar]
Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995.[PMC free article] [PubMed] [Google Scholar]
Reed KC, Mann DA. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221.[PMC free article] [PubMed] [Google Scholar]
Hu N, Messing J. The making of strand-specific M13 probes. Gene. 1982 Mar;17(3):271–277. [PubMed] [Google Scholar]
Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. [PubMed] [Google Scholar]
Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467.[PMC free article] [PubMed] [Google Scholar]
von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690.[PMC free article] [PubMed] [Google Scholar]
Sewell WA, Brown MH, Dunne J, Owen MJ, Crumpton MJ. Molecular cloning of the human T-lymphocyte surface CD2 (T11) antigen. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8718–8722.[PMC free article] [PubMed] [Google Scholar]
Kawata M, Parnes JR, Herzenberg LA. Transcriptional control of HLA-A,B,C antigen in human placental cytotrophoblast isolated using trophoblast- and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter. J Exp Med. 1984 Sep 1;160(3):633–651.[PMC free article] [PubMed] [Google Scholar]
Johansen KS, Johansen TS, Talmage DW. T cell rosette formation in primates, pigs, and guinea pigs. The influence of immunosuppresive agents. J Allergy Clin Immunol. 1974 Aug;54(2):86–93. [PubMed] [Google Scholar]
Nalet V, Fournier C. Human autologous rosette-forming cells. III. Binding of erythrocytes from different species to the T-cell receptors for autologous red blood cells. Cell Immunol. 1985 Nov;96(1):126–136. [PubMed] [Google Scholar]
Baxley G, Bishop GB, Cooper AG, Wortis HH. Rosetting of human red blood cells to thymocytes and thymus-derived cells. Clin Exp Immunol. 1973 Nov;15(3):385–392.[PMC free article] [PubMed] [Google Scholar]
Shaw S, Luce GE, Quinones R, Gress RE, Springer TA, Sanders ME. Two antigen-independent adhesion pathways used by human cytotoxic T-cell clones. Nature. 1986 Sep 18;323(6085):262–264. [PubMed] [Google Scholar]

Abstract