In vitro screening of traditionally used medicinal plants in China against enteroviruses.
Journal: 2006/October - World Journal of Gastroenterology
ISSN: 1007-9327
PUBMED: 16810764
Abstract:
OBJECTIVE
To search for new antiviral agents from traditional Chinese medicine, specifically anti-enteroviruses agents.
METHODS
The aqueous extracts (AE) of more than 100 traditionally used medicinal plants in China were evaluated for their in vitro anti-Coxsackie virus B3 activities with a MTT-based colorimetric assay.
RESULTS
The test for AE of 16 plants exhibited anti-Coxsackie virus B3 activities at different magnitudes of potency. They can inhibit three steps (inactivation, adsorption and replication) during the infection. Among the 16 plants, Sargentodoxa cuneata (Oliv.) Rehd. et Wils., Sophora tonkinensis Gapnep., Paeonia veitchii Lynch, Spatholobus suberectus Dunn. and Cyrtomium fortunei J. sm. also have activity against other enterovirus, including Coxsackie virus B5, Polio virus I, Echo virus 9 and Echo virus 29. Cell cytotoxic assay demonstrated that all tested AE had CC50 values higher than their EC50 values.
CONCLUSIONS
The sixteen traditionally used medicinal plants in China possessed antiviral activity, and some of them merit further investigations.
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World J Gastroenterol 12(25): 4078-4081

<em>In vitro</em> screening of traditionally used medicinal plants in China against Enteroviruses

Jin-Peng Guo, Ji Pang, Xin-Wei Wang, Zhi-Qiang Shen, Min Jin, Jun-Wen Li, Department of Environment and Health, Institute of Environment and Health, Tianjin 300050, China
Author contributions: All authors contributed equally to the work.

Correspondence to: Jun-Wen Li, Department of Environment and Health, Institute of Environment and Health, 1 Dali Road, Tianjin 300050, China. moc.uoye@ilnewnuj

Telephone: +86-22-84655418 Fax: +86-22-23328809

Jin-Peng Guo, Ji Pang, Xin-Wei Wang, Zhi-Qiang Shen, Min Jin, Jun-Wen Li, Department of Environment and Health, Institute of Environment and Health, Tianjin 300050, China
Author contributions: All authors contributed equally to the work.

Correspondence to: Jun-Wen Li, Department of Environment and Health, Institute of Environment and Health, 1 Dali Road, Tianjin 300050, China. moc.uoye@ilnewnuj

Telephone: +86-22-84655418 Fax: +86-22-23328809

Received 2005 Nov 21; Revised 2005 Dec 15; Accepted 2005 Dec 22.

Abstract

AIM: To search for new antiviral agents from traditional Chinese medicine, specifically anti-enterovirosuses agents.

METHODS: The aqueous extracts (AE) of more than 100 traditionally used medicinal plants in China were evaluated for their In vitro anti-Coxsackie virus B3 activities with a MTT-based colorimetric assay.

RESULTS: The test for AE of 16 plants exhibited anti-Coxsackie virus B3 activities at different magnitudes of potency. They can inhibit three steps (inactivation, adsorption and replication) during the infection. Among the 16 plants, Sargentodoxa cuneata (Oliv.) Rehd. et Wils., Sophora tonkinensis Gapnep., Paeonia veitchii Lynch, Spatholobus suberectus Dunn. and Cyrtomium fortunei J. sm. also have activity against other enterovirus, including Coxsackie virus B5, Polio virus I, Echo virus 9 and Echo virus 29. Cell cytotoxic assay demonstrated that all tested AE had CC50 values higher than their EC50 values.

CONCLUSION: The sixteen traditionally used medicinal plants in China possessed antiviral activity, and some of them merit further investigations.

Keywords: Traditional used medicinal plant, China, Antiviral activity, Enterovirus, Sargentodoxa cuneata (Oliv) Reld. et. Wils., Sophora tonkinensis Gapnep., Paeonia veitchii Lynch., Cyrtomium fortunei J. sm., Spatholobus suberectus Dunn
Abstract

++++: the strongest antiviral activity; +++: strong antiviral activity; ++: the modest antiviral activity; +: the weak antiviral activity; -: no antiviral activity.

Footnotes

S- Editor Wang J L- Editor Ma JY E- Editor Bi L

Footnotes

References

  • 1. Martino TA, Liu P, Petric M, Sole MJ. Enteroviral myocarditis and dilated cardiomyopathy: a rewiew of clinical and experimental studies. In: Rotbart HA, ed , editors. Human Enterovirus Infections. Washington DC: ASM Press; 1995. pp. 291–351. [PubMed]
  • 2. Sole MJ, Liu PViral myocarditis: a paradigm for understanding the pathogenesis and treatment of dilated cardiomyopathy. J Am Coll Cardiol. 1993;22:99A–105A.[PubMed][Google Scholar]
  • 3. Fujioka S, Kitaura YCoxsackie B virus infection in idiopathic dilated cardiomyopathy: clinical and pharmacological implications. BioDrugs. 2001;15:791–799.[PubMed][Google Scholar]
  • 4. Dalakas MC. Enteroviruses and human neuromuscular diseases. In: Rotbart HA, ed , editors. Human Enterovirus Infections. Washington DC: ASM Press; 1995. pp. 387–398. [PubMed]
  • 5. Rewers M, Atkinson M. The possible role of enteroviruses in diabetes mellitus. In: Rotbart HA, ed , editors. Human Enterovirus Infections. Washington DC: ASM Press; 1995. pp. 353–385. [PubMed]
  • 6. Muir P, Nicholson F, Illavia SJ, McNeil TS, Ajetunmobi JF, Dunn H, Starkey WG, Reetoo KN, Cary NR, Parameshwar J, et al Serological and molecular evidence of enterovirus infection in patients with end-stage dilated cardiomyopathy. Heart. 1996;76:243–249.[Google Scholar]
  • 7. Rotbart HATreatment of picornavirus infections. Antiviral Res. 2002;53:83–98.[PubMed][Google Scholar]
  • 8. Carrasco LPicornavirus inhibitors. Pharmacol Ther. 1994;64:215–290.[PubMed][Google Scholar]
  • 9. Loddo BDevelopment of drug resistance and dependence in viruses. Pharmacol Ther. 1980;10:431–460.[PubMed][Google Scholar]
  • 10. Nikolaeva L, Galabov ASIn vitro inhibitory effects of dual combinations of picornavirus replication inhibitors. Acta Virol. 1999;43:303–311.[PubMed][Google Scholar]
  • 11. Vlietinck AJ, Vanden Berghe DA, Haemers APresent status and prospects of flavonoids as anti-viral agents. Prog Clin Biol Res. 1988;280:283–299.[PubMed][Google Scholar]
  • 12. Tabba HD, Chang RS, Smith KMIsolation, purification, and partial characterization of prunellin, an anti-HIV component from aqueous extracts of Prunella vulgaris. Antiviral Res. 1989;11:263–273.[PubMed][Google Scholar]
  • 13. Cowan MMPlant products as antimicrobial agents. Clin Microbiol Rev. 1999;12:564–582.[Google Scholar]
  • 14. Kurokawa M, Ochiai H, Nagasaka K, Neki M, Xu H, Kadota S, Sutardjo S, Matsumoto T, Namba T, Shiraki KAntiviral traditional medicines against herpes simplex virus (HSV-1), poliovirus, and measles virus in vitro and their therapeutic efficacies for HSV-1 infection in mice. Antiviral Res. 1993;22:175–188.[PubMed][Google Scholar]
  • 15. Kaïj-a-Kamb M, Amoros M, Girre LSearch for new antiviral agents of plant origin. Pharm Acta Helv. 1992;67:130–147.[PubMed][Google Scholar]
  • 16. Li ZH, Nie BM, Chen H, Chen SY, He P, Lu Y, Guo XK, Liu JXIn vitro anti-coxsackievirus B(3) effect of ethyl acetate extract of Tian-hua-fen. World J Gastroenterol. 2004;10:2263–2266.[Google Scholar]
  • 17. Cavallaro U, Soria MRTargeting plant toxins to the urokinase and alpha 2-macroglobulin receptors. Semin Cancer Biol. 1995;6:269–278.[PubMed][Google Scholar]
  • 18. Vlietinck AJ, Vanden Berghe DACan ethnopharmacology contribute to the development of antiviral drugs. J Ethnopharmacol. 1991;32:141–153.[PubMed][Google Scholar]
  • 19. Baker JT, Borris RP, Carté B, Cordell GA, Soejarto DD, Cragg GM, Gupta MP, Iwu MM, Madulid DR, Tyler VENatural product drug discovery and development: new perspectives on international collaboration. J Nat Prod. 1995;58:1325–1357.[PubMed][Google Scholar]
  • 20. Krah DLA simplified multiwell plate assay for the measurement of hepatitis A virus infectivity. Biologicals. 1991;19:223–227.[PubMed][Google Scholar]
  • 21. Mosmann TRapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55–63.[PubMed][Google Scholar]
  • 22. Chiang LC, Chiang W, Chang MY, Ng LT, Lin CCAntiviral activity of Plantago major extracts and related compounds in vitro. Antiviral Res. 2002;55:53–62.[PubMed][Google Scholar]
  • 23. Chiang LC, Chiang W, Yu HS, Sheu HM, Chen HYEstablishment and characterization of a continuous human basal cell carcinoma cell line from facial skin (I) cytological behavior of early passages. Gaoxiong Yi Xue Ke Xue Za Zhi. 1994;10:170–176.[PubMed][Google Scholar]
  • 24. Cragg GM, Newman DJ, Snader KMNatural products in drug discovery and development. J Nat Prod. 1997;60:52–60.[PubMed][Google Scholar]
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