Efficient transformation of parahydrogen spin order into heteronuclear magnetization.
Journal: 2013/July - Journal of Physical Chemistry B
ISSN: 1520-5207
Abstract:
Spin order obtained in the strong coupling regime of protons from parahydrogen-induced hyperpolarization (PHIP) is initially captured as an ensemble of singlet states. For biomedical applications of PHIP, locking this spin order on long-lived heteronuclear storage nuclei increases spectral dispersion, reduces background interference from water protons, and eliminates the need to synchronize subsequent detection pulse sequences to accrued singlet-state evolution. A variety of traditional sequences such as INEPT or HMQC are available to interconvert heteronuclear single quantum coherences at high field, but new approaches are required for converting singlet states into heteronuclear single quantum coherences at low field in the strong coupling regime of protons. Described here is a consolidated pulse sequence that achieves this transformation of singlet-state spin order into heteronuclear magnetization across a wide range of scalar couplings in AA'X spin systems. Analytic solutions to the spin evolution are presented, and performance was validated experimentally in the parahydrogen addition product, 2-hydroxyethyl 1-(13)C-propionate-d(3). Hyperpolarized carbon-13 signals were enhanced by a factor of several million relative to Boltzmann polarization in a static magnetic field of 47.5 mT (~13% polarization). We anticipate that this pulse sequence will provide efficient conversion of parahydrogen spin order over a broad range of emerging PHIP agents that feature AA'X spin systems.
Relations:
Content
Citations
(17)
References
(15)
Grants
(52)
Chemicals
(1)
Processes
(1)
Affiliates
(1)
Similar articles
Articles by the same authors
Discussion board
J Phys Chem B 117(5): 1219-1224

Efficient Transformation of Parahydrogen Spin Order into Heteronuclear Magnetization

INTRODUCTION

Hyperpolarization of nuclear spin ensembles has increased NMR sensitivity to a level that is now enabling detection of metabolism in biological tissue on a time scale of seconds.1,2 The most developed of these technologies, DNP (dynamic nuclear polarization),35 in particular has already been used to detect, grade, and monitor response to therapy in tumors.68 These encouraging developments have demonstrated the overall viability of NMR-based hyperpolarized methods for the study of in vivo metabolism, and are naturally spurring development in alternative methods of hyperpolarization, such as parahydrogen-induced polarization (PHIP).911 Polarization yields from the less mature PHIP technology are similar to DNP where precursors are available, and accessed at significantly reduced instrumental complexity and expense. An array of complementary advances are still required, however, for PHIP to reach its potential as a diagnostic imaging modality.

In particular, efficient and streamlined methods for transforming the initial parahydrogen spin order into heteronuclear magnetization would improve translation of emerging PHIP contrast agents to biomedical applications. Upon addition of parahydrogen to a perdeuterated and unsaturated backbone containing a spin-active heteronucleus, the initial singlet state of an AA′X spin system will evolve unless JAX = JAX′. Transforming these states into longitudinal heteronuclear magnetization maximizes spectral dispersion during subsequent imaging experiments and reduces interference from the intense proton background arising from water in vivo. It has recently been demonstrated that parahydrogen singlet states can themselves be long-lived at Earth’s field,12 but even in cases where the parahydrogen proton lifetimes are similar to or even more favorable than carbonyl C for example, locking the initial spin order also eliminates the need to synchronize subsequent imaging with ongoing evolution of the initial parahydrogen singlet state.

Determining the timing, frequency, and magnitude of the applied electromagnetic fields necessary to efficiently transform parahydrogen spin order into heteronuclear magnetization in the strong coupling regime of protons is a challenging problem though, even for small AA′X spin systems. Two prior sequences have been reported for pulsed transformation of parahydrogen spin order into heteronuclear magnetization in this field regime.13,14 Most recently, Kadlecek and co-workers reported a series of sequences that yield piecewise optimal polarization in three distinct coupling regimes.13 The earlier and most frequently cited sequence developed by Goldman and coworkers offers unity efficiency for the targeted molecules and a recursive procedure for pumping polarization yields when outside of those coupling regimes.14 We sought to build on those earlier works by developing a streamlined sequence that could achieve optimal polarization in a single streamlined implementation across a broad range of scalar couplings without conditional or iterative application.

In this study, we describe a consolidated pulse sequence that transforms parahydrogen spin order into heteronuclear magnetization in AA′X spin systems with a yield near unity and independent of spin couplings. The shorthand hyper-SHIELDED (Singlet to Heteronuclei by Iterative Evolution Locks Dramatic Enhancement for Delivery) was adopted for quick referencing because the sequence effectively locks the spin order of hyperpolarized molecules for subsequent delivery and examination in biological or other systems of interest. The sequence provides near-unity conversion across a broad range of coupling topologies, by flanking a heteronuclear excitation with two asymmetric proton refocusing intervals to provide four unique evolution intervals. These delay intervals are in turn optimized using prior knowledge of the spin couplings to efficiently transform the initial parahydrogen spin order into pure heteronuclear magnetization. We anticipate that the streamlined construction of hyper-SHIELDED will be well suited to multidimensional PHIP experiments and for efficient preparation of existing and emerging PHIP contrast agents.

THEORY

The Hamiltonian of the three-spin system (AA′X = I1I2S) formed from the parahydrogen addition product (PASADE-NA)9,10 and a coupled heteronucleus in the strong proton coupling regime can be written as

H = 2π[J12(I1 · I2) + J1SI1zSz + J2SI2zSz]
(1)

The initial density matrix of parahydrogen at low field in the strong coupling regime of protons can be written:

σ0=E4-I1·I2
(2)

The I1zI2z component of this initial density matrix commutes with all components, and hence does not evolve under the Hamiltonian in eq 1. The remainder of the initial density matrix with hyper-SHIELDED (Figure 1) evolves during the interval t1 under the influence of the Hamiltonian according to the following expression:

σ1t1[sin2θ+(cos2θ)cos(2πΩt1)](I1xI2x+I1yI2y)
(3a)

+ (cosθ)sin(2πΩt1)2(I1yI2x - I1xI2y)Sz
(3b)

+ (sinθ)(cosθ)[1 - cos(2πΩt1)](I1z - I2z)Sz
(3c)

where cos θ = Δ/(1 + Δ), sin θ = 1/(1 + Δ), Δ = [J1sJ2s](2J12), and Ω = J12(1 + Δ). After applying a 180°(+x) pulse on protons, the density operator σ2 evolves according to

An external file that holds a picture, illustration, etc.
Object name is nihms652672f1.jpg

Graphical depiction of density matrix evolution (upper graph) with the hyper-SHIELDED sequence (lower graph). Para-hydrogen singlet states (represented by bold circles, middle left) are converted into pure heteronuclear magnetization (bold circle, middle right) for strongly coupled spin systems (I1I2S or AA′X). Circled symbols in the upper diagram represent density matrix terms and correspond directly with numbered equations (see Theory).

σ2t2[-cos2θ(sin2θ)+(1/2)sin22θ(cos(2πΩt1)+cos(2πΩt2))+cos2θ(cos2θ)cos(2πΩt1)cos(2πΩt2)+(cos2θ)sin(2πΩt1)sin(2πΩt2)](I1xI2x+I1yI2y)
(4a)
+[sin(2θ)sin(θ)sin(2πΩt2)+cos(2θ)cos(θ)cos(2πΩt1)sin(2πΩt2)-cos(θ)sin(2πΩt1)cos(2πΩt2)]2(I1yI2x-I1xI2y)Sz
(4b)
+[-(1/2)sin(2θ)(cos(2θ)+2sin2(θ)cos(2πΩt2)-2cos2(θ)cos(2πΩt1)+cos(2θ)cos(2πΩt1)cos(2πΩt2)+sin(2πΩt1)sin(2πΩt2))](I1z-I2z)Sz
(4c)

In order to drive the terms in eq 4 exclusively to eq 4c, the intervals t1 and t2 are chosen to satisfy eqs 5a and 5b:

-cos2θsin2θ+(1/2)sin22θ(cos(2πΩt1)+cos(2πΩt2))+cos(2θ)cos2θcos(2πΩt1)cos(2πΩt2)+cos2θsin(2πΩt1)sin(2πΩt2)=0
(5a)

sin(2θ)sin(θ)sin(2πΩt2)+cos(2θ)cos(θ)cos(2πΩt1)sin(2πΩt2)-cos(θ)sin(2πΩt1)cos(2πΩt2)=0
(5b)

A 90°(+y) pulse on the S spin then converts the state (I1z − I2z)Sz into (I1z − I2z)Sx. During the subsequent interval t3, this state evolves into three terms:

σ3=(I1z-I2z)Sxt3cos(2πΩt3)(I1z-I2z)Sx
(6a)

+ cos(θ)sin(2πΩt3)(1/2)Sy(E - 4I1zI2z)
(6b)

- sin(θ)sin(2πΩt3)2(I1yI2x - I1xI2y)Sx
(6c)

A proton 180(+x) pulse is then applied, and during the subsequent interval t4, the density matrix evolves according to the expressions:

σ4t4[cos(2πΩt3)cos(2πΩt4)+cos(2θ)sin(2πΩt3)sin(2πΩt4)](I1z-I2z)Sx
(7a)

+[cos(θ)cos(2πΩt3)sin(2πΩt4)-sin(2θ)sin(θ)sin(2πΩt3)-cos(θ)cos(2θ)sin(2πΩt3)cos(2πΩt4)](1/2)Sy(E-4I1zI2z)
(7b)

+[sin(θ)cos(2θ)sin(2πΩt3)cos(2πΩt4)-sin(θ)cos(2πΩt3)sin(2πΩt4)-sin(2θ)cos(θ)sin(2πΩt3)]2(I1yI2x-I1xI2y)Sx
(7c)

where E equals the identity operator. The intervals t3 and t4 are chosen to satisfy the following set of equations:

cos(2πΩt3)cos(2πΩt4) + cos(2θ)sin(2πΩt3)sin(2πΩt4) = 0
(8a)

sin(θ)cos(2θ)sin(2πΩt3)cos(2πΩt4)-sin(θ)cos(2πΩt3)sin(2πΩt4)-sin(2θ)cos(θ)sin(2πΩt3)=0
(8b)

This condition is satisfied when

tan(2πΩt3)=-1/1+2cos(2θ)
(9a)
tan(2πΩt4)=1/1+2cos(2θ)
(9b)

Finally, a 90°(+x) pulse converts the term from eq 7b into longitudinal magnetization on the heteronucleus for storage until subsequent detection in vivo. The pulse sequence diagram and schematic of spin evolution represented by eqs 27 is illustrated in Figure 1.

EXPERIMENTAL SECTION

Approximately 98% parahydrogen gas was synthesized by pulsing ambient research grade hydrogen gas at 14 bar (200 psi) into a catalyst-filled (iron oxide) copper chamber held at 14 K using a previously described semiautomated parahydrogen generator.15 Fresh batches of parahydrogen were collected in 10 L aluminum storage tanks (14745-SHF-GNOS, Holley, KY), used without Teflon lining or additional modification.

The preparation of PASADENA9,10 precursor aqueous solutions was similar to those previously described16 with the exception that water was used in place of 99.8% D2O as a solvent. 320 μmol (0.180 g) of the disodium salt of 1,4-bis[(phenyl-3-propanesulfonate)phosphine]butane (#717347, Sigma-Aldrich-Isotec, OH) was combined with 100 mL of H2O in a 1 L flask. This ambient solution was then degassed with a rotary evaporator (model R-215 equipped with V-710 pump, Buchi, New Castle, DE) fitted with an N2(g) input, by decrementing the onboard pressure slowly to avoid boiling, from 70 to 25 mbar over approximately 10 min. The rhodium(I) catalyst, bis(norbornadiene) rhodium(I) tetrafluoroborate (0.10 g, 0.27 mmol, 45-0230, CAS 36620-11-8, Strem Chemicals, MA) was dissolved in 7 mL of acetone and was added dropwise to the phosphine ligand solution to limit undesirable precipitation. After the prior degassing procedure was repeated, this catalyst solution was mixed with 2-hydroxyethyl acrylate-1-C,2,3,3-d3 (HEA, 97% chemical purity, 99 atom % C, 98 atom % D (20 mg, 0.16 mmol, Sigma-Aldrich, 676071) in a 150 mL square bottle (431430, Corning Life Sciences, NY).

Solutions containing unsaturated precursor molecules with bidentate Rh(I) catalyst prepared as described above were then connected to a previously described automated parahydrogen polarizer,16 equipped with a dual-tuned H/C coil.17 Briefly, the chemical reaction was pulse programmed with a commercial NMR console (model KEA2, Magritek, Wellington, New Zealand) to synchronize chemical reaction parameters, decoupling fields, polarization transfer sequences, and detection of NMR signals. PASADENA precursors were sprayed remotely into a plastic (polysulfone) reactor located within a 47.5 mT static magnetic field. The external solution was equilibrated at 65 °C prior to spraying, and 16.5 bar (240 psi) nitrogen gas was used to inject this heated PASADENA precursor solution into a pressurized atmosphere of 7 bar (100 psi) parahydrogen. Immediately following injection, proton continuous wave decoupling was applied at a frequency of 2.02 MHz (B0 = 47.5 mT) with a magnitude of 5 kHz. This decoupling radio frequency field was maintained for 4 s to freeze the parahydrogen spin ensemble while the hydrogenation reaction went to completion.14

The pulse sequences for transferring polarization were applied immediately after continuous wave decoupling was turned off (Figure 1). For the HEP molecule, the t1, t2, t3, and t4 intervals were 9.75, 58.47, 36.20, and 28.28 ms, respectively, calculated by inverting the density matrix expressions above (see Theory) assuming a proton–proton coupling of 7.57 Hz, and a carbon–proton scalar coupling asymmetry of 12.86 Hz.14 The actual couplings could vary somewhat from these values depending on pH and specific attributes of the polarization process such as temperature and pressure. After parahydrogen spin order was transferred to net magnetization, a single free induction decay was acquired (90–acquire) on the carbon channel with 512 points at a receiver bandwidth of 5 kHz, for a digital resolution of ~10 Hz per point.

RESULTS AND DISCUSSION

Described here is a new pulse sequence (hyper-SHIELDED) for transforming parahydrogen spin order in the strong coupling regime of protons into net heteronuclear magnetization. Hyper-SHIELDED operates at nearly unity efficiency with yields that are approximately independent of scalar coupling topology in three spin systems (AA′X). The AA′X moiety is a widespread and important spin system in PHIP experiments formed for example, by molecular addition of parahydrogen to perdeuterated and unsaturated molecular backbones.

Hyper-SHIELDED flanks two asymmetric proton refocusing intervals about a heteronuclear excitation pulse to generate four unique delays (t1t4). Optimization of these delays to spin couplings in the molecule of interest sequentially converts the initial parahydrogen singlet state into pure heteronuclear magnetization (see Theory, Figure 1). Density matrix evolution under the influence of hyper-SHIELDED is depicted graphically in Figure 1 and linked directly to equations in the Theory section.

The analysis of spin dynamics under the influence of hyper-SHIELDED assumed strongly coupled protons and weak heteronuclear scalar couplings (eq 1). The initial parahydrogen density operator was retained without truncation and proportional to I1·I2 (eq 2). Chemical shifts were not considered because the effects are small compared to homonuclear proton couplings at targeted fields in the vicinity of 47.5 mT or lower, and we note that offsets were refocused with 180° pulses on both channels placed at 1/4 and 3/4 of each evolution interval.14 Evolution of the strongly coupled parahydrogen density operator of eq 2 is relatively complicated compared to (truncated) high field density operators proportional to I1zI2z. While analytical solutions to the spin dynamics are more tedious, heteronuclear magnetization yields from parahydrogen spin order are increased by a factor of 2 at low field in the strong coupling regime of protons.

Hyper-SHIELDED was applied immediately following the hydrogenation reaction. During the fast catalytic hydrogenation,9 proton decoupling was used to maintain equivalence of the parahydrogen protons and freeze evolution of the spin density operator until reaction completion (Figure 1).18 After this period of decoupling and chemical addition, with hyper-SHIELDED the initial density matrix evolved from the parahydrogen singlet state (eq 2) to three terms (eqs 3a3c, symbols 3a–c in Figure 1) in the Cartesian product basis19 during the first interval (t1). A 180°(x) proton pulse then focused these three terms of the density matrix into term 4c during the interval t2. A 90°(y) pulse on the S-nucleus then allowed term 4c to evolve into an additional three terms (symbols 6a–c, Figure 1) during the interval t3. Following a proton 180° pulse, these three terms (symbols 6a–c, Figure 1) collapse into a single term during t4 (symbol 7b, Figure 1).

Note that since I1zI2z commutes with the Hamiltonian, I1zI2z(t=0) = I1zI2z(t). Since Iz1(t=0) + Iz2(t=0) = 0 for the parahydrogen singlet state, 4I1zI2z reduces to −E. Therefore, when the evolution intervals are chosen to satisfy eqs 8a and 8b, eq 7b reduces to a pure Sy term. Rotating this heteronuclear magnetization then locks the original parahydrogen spin order along Sz, where it will persist according to relaxation kinetics specific to the storage nucleus. Alternatively, if left unperturbed in the transverse plane this term could be detected directly at the field where the PHIP preparation was performed.16 Nonselective refocusing pulses were interleaved at 1/4 and 3/4 on both channels in each evolution interval to refocus offsets and mitigate the deleterious impact of static field inhomogeneities.14

Two prior sequences have been reported to transform parahydrogen spin order into heteronuclear magnetization in the strong proton coupling regime where the process is most efficient.13,14 Most recently, Kadlecek and co-workers reported a set of sequences that can be selectively applied to yield optimal transfer efficiency in three distinct scalar coupling regimes.13 Goldman and co-workers reported the first pulsed transfer method which yields near unity singlet-state transformation efficiency in proximity to the scalar couplings of the design molecule. They also described a recursion procedure to pump polarization yields with GPS toward unity when outside of those targeted coupling regimes.14 With hyper-SHIELDED, we sought to build on these efficient earlier works by developing a sequence that could achieve optimal conversion efficiency in a single streamlined sequence without recursive application and with minimal sensitivity to scalar coupling.

To characterize sensitivity of hyper-SHIELDED to scalar couplings, transfer efficiency was calculated with respect to proton–proton scalar couplings (J12) and coupling asymmetry (|J1SJ2S|) over a range spanning known and conceivable PHIP reaction products (Figures 2 and and3).3). For each unique set of couplings (J12, |J1SJ2S|), the set of evolution intervals yielding maximum efficiency was determined by inverting the density matrix equations subject to a 300 ms total sequence duration constraint (see Theory). As illustrated in Figure 3, a broad plateau of unity transformation efficiency was obtained with as little as ~6 Hz heteronuclear coupling asymmetry (|J1SJ2S|) and ~2 Hz homonuclear proton coupling (J12). If application warranted and relaxation times were favorable, expanding the total pulse sequence duration constraint beyond 300 ms would enable sharper transitions from valley to plateau.

An external file that holds a picture, illustration, etc.
Object name is nihms652672f2.jpg

Polarization yield as a function of homonuclear proton coupling (J12) and heteronuclear coupling asymmetry (|J1SJ2S|) for hyper-SHIELDED. Contour levels are marked at right and superposed onto a gradient map calculated at an isotropic resolution of 0.1 Hz. For each point, the density matrix equations were inverted to find τ intervals (ti, Figure 1) corresponding to maximum polarization and normalized to the global maximum. Annotated coordinates refer to the test molecule (HEP) and a sample of N parahydrogen addition products with small asymmetries that would be expected to differentially benefit from hyper-SHIELDED.

An external file that holds a picture, illustration, etc.
Object name is nihms652672f3.jpg

Dependence of theoretical polarization transfer efficiency on heteronuclear coupling constant asymmetry |J1SJ2S| using the hyper-SHIELDED sequence (solid) versus a nonrecursive implementation of the GPS comparison sequence.14 The τ intervals were optimized to produce maximum polarization for each sequence at a common value of J12 of 7.5 Hz.

To validate the sequence, experimental heteronuclear C signals were compared between hyper-SHIELDED and GPS14 for a PHIP reaction product where both sequences were predicted to perform with identical efficiency (Figures 3 and and4).4). As shown in Figure 4 and in accord with theoretical expectations, C magnetization yield in a 7 μmol sample of the PHIP reaction product, 2-hydroxyethyl 1-C-propionate-d3, was enhanced by a large and equivalent factor of several million with both sequences. Although not yet experimentally demonstrated or implemented on our equipment, the piecewise optimal sequences recently reported by Kadlecek and coworkers would likely offer similarly efficient results.13 Whereas the current sequence (hyper-SHIELDED) and earlier sequences all appear to be well-suited to transforming parahydrogen spin order efficiently into net heteronuclear magnetization, we sought to complement the earlier advances13,14 by creating a single streamlined sequence that could achieve high transfer efficiencies independent of scalar couplings. Implementation of hyper-SHIELDED is experimentally compact and because the sequence does not rely on condition or recursive application for broadband efficiency, it can be readily extended to multidimensional experiments on mixtures containing molecules with a range of couplings.

An external file that holds a picture, illustration, etc.
Object name is nihms652672f4.jpg

Polarization yield of hyper-SHIELDED sequence versus GPS.14 (a) Boltzmann polarized carbon-13 spectrum acquired from an aqueous solution containing 170 mmol of the reaction product. (b) Reaction schematic for synthesizing parahydrogenated 2-hydroxyethyl 1-C-propionate-d3 (HEP). (c) Experimentally determined yields for hyper-SHIELDED and GPS comparison sequence were nearly identical and in accord with theory for the coupling arrangement in the test HEP molecule.

Theoretical conversion efficiency was also analyzed at a specific J12 (7.5 Hz) and compared to the nonrecursive application of GPS. As illustrated in Figure 2, the dependence of polarization yield in the small asymmetry regime is relatively insensitive to J12. Polarization yields reach uniform efficiency more rapidly as a function of asymmetry in hyper-SHIELDED versus the nonrecursive application of GPS,18 and high levels of polarization are sustained across a broad range of asymmetries (Figure 3). Although the calculated data points in Figure 3 were not parsed by sequence duration, hyper-SHIELDED was slightly longer (17.88 ms) at the HEP optimum. For the C relaxation constants of HEP, the increased duration of the hyper-SHIELDED sequence did not reduce polarization yield (Figure 4). Hyper-SHIELDED should perform particularly well in molecules with small asymmetries such as ethylamine, diethyamine, and choline (Figure 3 inset).20,21

CONCLUSIONS

A new pulse sequence was presented for transforming singlet-state spin order in the strong coupling regime of protons into heteronuclear magnetization. Conversion efficiency with this sequence (hyper-SHIELDED) is uniform and approximately independent over a broad range of scalar coupling patterns, and these attributes are obtained in a single consolidated sequence without recursion by invoking four independently optimized evolution delays. Analytical solutions to density matrix evolutions were found sequentially for each interval, and performance was validated experimentally with in situ detection of hyperpolarized 2-hydroxyethyl 1-C-propionate-d3. Locking parahydrogen spin order efficiently in the form of heteronuclear magnetization in a flexible array of scalar coupling topologies is important for translating parahydrogen-based hyperpolarization from basic science to emerging applications in biomedicine, and we anticipate that this sequence will be generally useful for a range of emerging PHIP contrast agents targeted for in vivo metabolism.

Acknowledgments

We gratefully acknowledge public funding support from ICMIC 5P50 CA128323-03, 5R00 CA134749-03, R25 CA136440, and 3R00CA134749-02S1.

Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee 37232-2310, United States
Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee 37232-2310, United States
Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, Tennessee 37232-2310, United States
Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, Tennessee 37232-2310, United States
Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232-2310, United States
Corresponding Author: ude.tlibrednav@lleddaw.nivek

Abstract

An external file that holds a picture, illustration, etc.
Object name is nihms652672u1.jpg

Spin order obtained in the strong coupling regime of protons from parahydrogen-induced hyperpolarization (PHIP) is initially captured as an ensemble of singlet states. For biomedical applications of PHIP, locking this spin order on long-lived heteronuclear storage nuclei increases spectral dispersion, reduces background interference from water protons, and eliminates the need to synchronize subsequent detection pulse sequences to accrued singlet-state evolution. A variety of traditional sequences such as INEPT or HMQC are available to interconvert heteronuclear single quantum coherences at high field, but new approaches are required for converting singlet states into heteronuclear single quantum coherences at low field in the strong coupling regime of protons. Described here is a consolidated pulse sequence that achieves this transformation of singlet-state spin order into heteronuclear magnetization across a wide range of scalar couplings in AA′X spin systems. Analytic solutions to the spin evolution are presented, and performance was validated experimentally in the parahydrogen addition product, 2-hydroxyethyl 1-C-propionate-d3. Hyperpolarized carbon-13 signals were enhanced by a factor of several million relative to Boltzmann polarization in a static magnetic field of 47.5 mT (~13% polarization). We anticipate that this pulse sequence will provide efficient conversion of parahydrogen spin order over a broad range of emerging PHIP agents that feature AA′X spin systems.

Abstract

Footnotes

Notes

The authors declare no competing financial interest.

Footnotes

References

  • 1. Ardenkjaer-Larsen JH, Fridlund B, Gram A, Hansson G, Hansson L, Lerche MH, Servin R, Thaning M, Golman K. Proc Natl Acad Sci USA. 2003;100:10158–10163.
  • 2. Golman K, Zandt RI, Lerche M, Pehrson R, Ardenkjaer-Larsen JH. Cancer Res. 2006;66:10855–60.[PubMed]
  • 3. Carver TR, Slichter CP. Phys Rev. 1953;92:212.[PubMed]
  • 4. Carver TR, Slichter CP. Phys Rev. 1956;102:975.[PubMed]
  • 5. Overhauser AW. Phys Rev. 1953;92:411.[PubMed]
  • 6. Albers MJ, Bok R, Chen AP, Cunningham CH, Zierhut ML, Zhang VY, Kohler SJ, Tropp J, Hurd RE, Yen YF, Nelson SJ, Vigneron DB, Kurhanewicz J. Cancer Res. 2008;68:8607–15.
  • 7. Day SE, Kettunen MI, Gallagher FA, Hu DE, Lerche M, Wolber J, Golman K, Ardenkjaer-Larsen JH, Brindle KM. Nat Med. 2007;13:1382–7.[PubMed]
  • 8. Golman K, Petersson JS. Acad Radiol. 2006;13:932–42.[PubMed]
  • 9. Bowers CR, Weitekamp DP. J Am Chem Soc. 1987;109:5541–5542.[PubMed]
  • 10. Bowers CR, Weitekamp DP. Phys Rev Lett. 1986;57:2645–2648.[PubMed]
  • 11. Adams RW, Aguilar JA, Atkinson KD, Cowley MJ, Elliott PI, Duckett SB, Green GG, Khazal IG, Lopez-Serrano J, Williamson DC. Science. 2009;323:1708–11.[PubMed]
  • 12. Reineri F, Santelia D, Gobetto R, Aime S. J Magn Reson. 2009;200:15–20.[PubMed]
  • 13. Kadlecek S, Emami K, Ishii M, Rizi R. J Magn Reson. 2011;205:9–13.
  • 14. Goldman M, Johannesson H, Axelsson O, Karlsson M. Magn Reson Imaging. 2005;23:153–7.[PubMed]
  • 15. Feng BB, Coffey AM, Colon RD, Chekmenev EY, Waddell KW. J Magn Reson. 2012;214:258–262.
  • 16. Waddell KW, Coffey AM, Chekmenev EY. J Am Chem Soc. 2011;133:97–101.
  • 17. Coffey AM, Shchepin RV, Wilkens K, Waddell KW, Chekmenev EY. J Magn Reson. 2012;220:94–101.
  • 18. Goldman M, Johannesson H. Compt Rend Phys. 2005;6:575–581.[PubMed]
  • 19. Sorensen OW, Eich GW, Levitt MH, Bodenhausen G, Ernst RR. Prog Nucl Magn Reson Spectrosc. 1983;16:163–192.[PubMed]
  • 20. Sarkar R, Comment A, Vasos PR, Jannin S, Gruetter R, Bodenhausen G, Hall Hln, Kirik D, Denisov VP. J Am Chem Soc. 2009;131:16014–16015.[PubMed]
  • 21. Reineri F, Viale A, Ellena S, Alberti D, Boi T, Giovenzana GB, Gobetto R, Premkumar SSD, Aime S. J Am Chem Soc. 2012;134:11146–11152.[PubMed]
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.