Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2.
Journal: 2005/July - Blood
ISSN: 0006-4971
Abstract:
Homing of mast cell progenitors (MCps) to the mouse small intestine involves the interaction of alpha4beta7 integrin with mucosal addressin cellular adhesion molecule-1 (MAdCAM-1). We now demonstrate the dependence of this process on CXC chemokine receptor 2 (CXCR2) and vascular cell adhesion molecule-1 (VCAM-1) using null strains and mice sublethally irradiated and bone marrow (BM) reconstituted (SIBR) with wild-type or null BM or with wild-type BM followed by administration of blocking antibody. The intestinal MCp concentration in CXCR2(-/-) mice was reduced by 67%, but was unaltered in CC chemokine receptor 2(-/-) (CCR2(-/-)), CCR3(-/-), or CCR5(-/-) mice. SIBR mice given CXCR2(-/-) BM had an intestinal MCp concentration that was 76% less than that in BALB/c BM reconstituted mice. Antibody blockade of VCAM-1 or of CXCR2 in SIBR mice reduced intestinal MCp reconstitution, and mice lacking endothelial VCAM-1 also had a marked reduction relative to wild-type mice. Finally, the half-life of intestinal MCps in wild-type mice was less than one week on the basis of a more than 50% reduction by administration of anti-alpha4beta7 integrin or anti-CXCR2. Thus, the establishment and maintenance of MCps in the small intestine is a dynamic process that requires expression of the alpha4beta7 integrin and the alpha-chemokine receptor CXCR2.
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Blood 105(11): 4308-4313

Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2

From the Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH; the Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA; the Molecular Immunology Program, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA; Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT; and Autoimmune and Inflammatory Diseases, Protein Design Labs, Fremont, CA.
Reprints: Michael F. Gurish, Smith Research Bldg, Room 616D, One Jimmy Fund Way, Boston, MA 02115-6085; e-mail: ude.dravrah.hwb.scir@hsirugm.
Reprints: Michael F. Gurish, Smith Research Bldg, Room 616D, One Jimmy Fund Way, Boston, MA 02115-6085; e-mail: ude.dravrah.hwb.scir@hsirugm.
Received 2004 Sep 15; Accepted 2005 Jan 26.

Abstract

Homing of mast cell progenitors (MCps) to the mouse small intestine involves the interaction of α4β7 integrin with mucosal addressin cellular adhesion molecule-1 (MAdCAM-1). We now demonstrate the dependence of this process on CXC chemokine receptor 2 (CXCR2) and vascular cell adhesion molecule-1 (VCAM-1) using null strains and mice sublethally irradiated and bone marrow (BM) reconstituted (SIBR) with wild-type or null BM or with wild-type BM followed by administration of blocking antibody. The intestinal MCp concentration in CXCR2 mice was reduced by 67%, but was unaltered in CC chemokine receptor 2 (CCR2), CCR3, or CCR5 mice. SIBR mice given CXCR2 BM had an intestinal MCp concentration that was 76% less than that in BALB/c BM reconstituted mice. Antibody blockade of VCAM-1 or of CXCR2 in SIBR mice reduced intestinal MCp reconstitution, and mice lacking endothelial VCAM-1 also had a marked reduction relative to wild-type mice. Finally, the half-life of intestinal MCps in wild-type mice was less than one week on the basis of a more than 50% reduction by administration of anti-α4β7 integrin or anti-CXCR2. Thus, the establishment and maintenance of MCps in the small intestine is a dynamic process that requires expression of the α4β7 integrin and the α-chemokine receptor CXCR2.

Abstract

Values are the mean MCp concentration (± SEM for n = 3, or ½ range for n = 2) in the indicated tissue from the various mice. Deficient mice and their wild-type controls (listed directly below) were evaluated in parallel. The number of experiments is indicated in parentheses.

ND indicates not determined.

Notes

Prepublished online as Blood First Edition Paper, February 10, 2005; DOI 10.1182/blood-2004-09-3578.

Supported by grants from the National Institutes of Health: AI-057991, HL-036110, AI-031599, and AI-047379.

The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 U.S.C. section 1734.

Notes
Prepublished online as Blood First Edition Paper, February 10, 2005; DOI 10.1182/blood-2004-09-3578.
Supported by grants from the National Institutes of Health: AI-057991, HL-036110, AI-031599, and AI-047379.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 U.S.C. section 1734.

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