Cadherin-4 plays a role in the development of zebrafish cranial ganglia and lateral line system.
Journal: 2007/July - Developmental Dynamics
ISSN: 1058-8388
Abstract:
We previously reported that cadherin-4 (also called R-cadherin) was expressed by the majority of the developing zebrafish cranial and lateral line ganglia. Cadherin-4 (Cdh4) function in the formation of these structures in zebrafish was studied using morpholino antisense technology. Differentiation of the cranial and lateral line ganglia and lateral line nerve and neuromasts of the cdh4 morphants was analyzed using multiple neural markers. We found that a subset of the morphant cranial and lateral line ganglia were disorganized, smaller, with reduced staining, and/or with altered shape compared to control embryos. Increased cell death in the morphant ganglia likely contributed to these defects. Moreover, cdh4 morphants had shorter lateral line nerves and a reduced number of neuromasts, which was likely caused by disrupted migration of the lateral line primordia. These results indicate that Cdh4 plays a role in the normal formation of the zebrafish lateral line system and a subset of the cranial ganglia.
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Dev Dyn 236(3): 893-902

Cadherin-4 Plays a Role in the Development of Zebrafish Cranial Ganglia and Lateral line System

Department of Biology, University of Akron, Akron, Ohio 44325-3908
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-0616
Department of Medicine, Indiana University Medical Center, Indianapolis, Indiana 46202
To whom correspondence should be addressed. Phone: 330-972-7558; Fax: 330-972-8445; E-mail: ude.norkau@uilq

Abstract

We previously reported that cadherin4 (also called R-cadherin) was expressed by the majority of the developing zebrafish cranial and lateral line ganglia. Cadherin4 function in the formation of these structures in zebrafish was studied using morpholino antisense technology. Differentiation of the cranial and lateral line ganglia, and lateral line nerve and neuromasts of the cadherin4 morphants was analyzed using multiple neural markers. We found that a subset of the morphants cranial and lateral line ganglia were disorganized, smaller, with reduced staining, and/or with altered shape compared to control embryos. Increased cell death in the morphant ganglia likely contributed to these defects. Moreover, cadherin4 morphants had shorter lateral line nerves and reduced number of neuromasts, which was likely caused by disrupted migration of the lateral line primordia. These results indicate that cadherin4 plays a role in the normal formation of the zebrafish lateral line system and a subset of the cranial ganglia.

Keywords: zebrafish, development, lateral line nerve, neuromasts, cell adhesion molecules, cranial nerves
Abstract
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