Anti-proliferative and antioxidant activities of Saposhnikovia divaricata.
Journal: 2007/August - Oncology Reports
ISSN: 1021-335X
PUBMED: 17549372
Abstract:
The dry root of Saposhnikovia divaricata (Turcz.) Schischk. (SD, syn. Ledebouriella divaricata (Turcz.); Umbelliferae), Siler, a perennial herb of the carrot family, is also known as Fang Feng in traditional Chinese herbal medicine. It is a herbal ingredient included in many polyherb formulae. This study investigated the in vitro anti-proliferative, antioxidant and anti-inflammatory activities of the SD extract (1 g/10 ml 70% ethanol). IC50 (50% inhibition) is estimated at 1/300, 1/1400, 1/250 and 1/600 dilutions, for the K562, HL60, MCF7 and MDA-MB-468 cell lines, respectively. The combination of non-cytotoxic concentrations of SD with chemotherapeutic drugs such as camptothecin or paclitaxel showed additive anti-proliferative effects on K562, HL60 and MCF7 cells, and antagonistic effects on MDA-MB-468 cells. At a dilution of 1/2000, SD induced a differentiation of 17.5+/-2.5% in HL60 cells along the granulocyte lineage compared to 2.8+/-0.8% in the untreated controls, but not along the monocyte/macrophage lineage. At non-cytotoxic 1/10000, 1/5000 and 1/2000 dilutions, the SD extract did not affect nitric oxide (NO) production by non-stimulated RAW 264.7 cells, but dose-dependently and significantly reduced NO production by lipopolysaccharide (LPS)-activated RAW 264.7 cells. RT-PCR analyses showed that SD at a dilution of 1/2000 did not affect TNFalpha, IL-1 beta, iNOS and COX-2 mRNA expression in RAW 264.7 cells compared to the unstimulated controls, but significantly reduced (p<0.05) iNOS and its mRNA expression in LPS-activated cells. It is concluded that the SD ethanol extract possesses strong anti-proliferative properties against several human tumor cell lines, a mild granulocyte differentiation inducing property on HL60 cells, and potent antioxidant, anti-inflammatory and protective properties on LPS-activated RAW 264.7 cells. Further research is required in order to identify the major ingredients present in the Saposhnikovia divaricata root and rhizome showing the observed activities.
Relations:
Citations
(9)
Drugs
(1)
Chemicals
(7)
Organisms
(4)
Processes
(3)
Anatomy
(6)
Affiliates
(1)
Similar articles
Articles by the same authors
Discussion board
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.