A probable novel splicing isoform of human vascular endothelial growth factor.
Journal: 2013/May - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
ISSN: 1673-4254
PUBMED: 22699049
Abstract:
OBJECTIVE
To characterize a new alternative splicing isoform of human vascular endothelial growth factor (VEGF) gene.
METHODS
The total RNA was extracted from the lung tissue of a legally aborted 4-month-old fetus and amplified by RT-PCR. The amplified product was cloned into the plasmid pMD18-T and plasmid pcDNA3.1- for sequence analysis.
RESULTS
Electrophoresis of the RT-PCR products displayed one short band for VEGF(121) (487 bp) and a long band. The latter was characterized to contain two fragments: one was normal VEGF(165) (619 bp), and the other (639 bp) had an identical nucleotide sequence to VEGF(165) with a 20 bp fragment inserted between exons 3 and 4. Sequence analysis showed that this 20-bp nucleotide was inserted from the 3' end of the third intron containing a splicing signal, thus causing shift mutation in the reading frame of VEGF gene and early appearance of the stop codon UAG in the middle of exon 4.
CONCLUSIONS
A new alternative splicing isoform of VEGF probably exists in the lung tissue of a legally aborted human fetus, and its biological significance remains to be further investigated.
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